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主要应用 CULT 描述 产品目录编号 CC145 品牌系列 Chemicon®
商名 - Chemicon
描述 Rat Laminin-5 概述 Laminin-5 is a basement membrane extracellular matrix macromolecule that provides an attachment substrate for both adhesion and migration in a wide variety of cell types, including epithelial cells, fibroblasts, neurons and leukocytes. It is a natural substrate for cells in contact with basement membranes, including epidermal and oral keratinocytes, gastro-intestinal tract, mammary and prostate epithelial cells. These cells, compared to fibronectin, collagen, or vitronectin, will adhere to Laminin-5 faster and spread to a larger extent. Laminin-5 can be used, in most applications, at coating concentrations in the 1 mg/ml or lower range, approximately 10-fold lower than most extracellular matrix macromolecules. Laminin-5 is attracting attention as a substrate that may stimulate carcinoma cell migration, since it has been detected at the leading edge of invasive cancer tissue in several types of human carcinomas. Receptors for Laminin-5 include integrins a3b1 and a6b4. The latter integrin is specialized in forming hemidesmosomes, which provide resistance to mechanical stress in many epithelia, including skin and gut. More recently, cell surface proteoglycans, such as syndecan-4, have been identified as receptors for Laminin-5. The structural and functional properties of Laminin-5 are well conserved across species. For instance, human cells adhere equally well to human and rat laminin-5. For most purposes, human and rat Laminin-5 are interchangeable, likely based on the fact that integrin receptor specificity is largely conserved across species. 产品信息 演示 Purified protein supplied in 20mM Tris, pH 7.5, 0.005% Brij-35, and 1% CHAPS containing no preservatives. 应用 主要应用 - Cell Culture
应用说明 Recommended coating concentrations: 1 μg/mL for cell adhesion assays, 0.2 μg/mL for cell migration assays.
Optimal dilutions must be determined by end user生物信息 浓缩 Variable 纯度 >95% as determined by SDS-PAGE 来源 Rat bladder carcinoma cell line 804G {Koshikawa, N et al. (2000) J. Cell Biol. 148:615-624. 品种 Rat UniProt编号 - P70636
产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -70°C in undiluted aliquots for up to 12 months from date of receipt. Avoid repeated freeze/thaw cycles. 包装信息 数量 10 µg
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