merck millipore,默克密理博,324867,PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb

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merck millipore,默克密理博,324867,PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb
产品名称：PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb
产品型号：324867
This PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb is validated for use in Immunoblotting for the detection of EGFR (pTyr¹⁰⁶⁸).

merck millipore,默克密理博,324867,PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb

产品介绍

merck millipore,默克密理博,324867,PhosphoDetect™ Anti-EGFR (pTyr¹⁰⁶⁸) Rabbit pAb

重要规格表

宿主
Rb

描述
概述	Recognizes the ~185 kDa EGFR protein phosphorylated at Tyr¹⁰⁶⁸. Note: 1 T = 1 test. Sufficient for 10 Western miniblots.
产品目录编号	324867
品牌系列	Calbiochem®
同义词	Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸)
应用数据	Detection of human EGFR, phosphorylated at Tyr¹⁰⁶⁸, by immunoblotting. Samples: Whole cell lysate from A431 cells unstimulated (lane 1), or stimulated for EGF (lanes 2-5). Primary antibody: PhosphoDetect™ Anti-Epidermal Growth Factor Receptor (pTyr¹⁰⁶⁸) Rabbit pAb (Cat. No. 324867) (1:1000) pre-incubated without peptide (lanes 1 and 5), with phosphopeptide immunogen (lane 2), a generic phosphotyrosine-containing peptide (lane 3), or non-phosphopeptide corresponding to the immunogen (lane 4). Detection: chemiluminescence.

参考
参考	Jo, M., et al. 2000. J. Biol. Chem. 275, 8806. Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572. Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249. Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067. Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139 Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227. Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.

参考

Jo, M., et al. 2000. J. Biol. Chem. 275, 8806.
Maudsley, S., et al. 2000. J. Biol. Chem. 275, 9572.
Sieg, D.J. et al. 2000. Nat. Cell. Biol. 2, 249.
Barbier, A.J. et al. 1999. J. Biol. Chem. 274, 14067.
Hashimoto, A. et al. 1999. J. Biol. Chem. 274, 20139
Poppleton, H.M. et al. 1999. Arch. Biochem. Biophys. 363, 227.
Batzer, A.G. et al. 1994. Mol. Cell. Biol. 14, 5192.

产品信息
形式	Liquid
配方	In Dulbecco's PBS, 1 mg/ml BSA, 50% glycerol, pH 7.4.
防腐剂	≤0.1% sodium azide

应用
主要应用	Immunoblotting (Western Blotting)

生物信息
免疫原	a synthetic phosphopeptide corresponding to amino acids surrounding the Tyr¹⁰⁶⁸ phosphorylation site of human EGFR
免疫原品种	Human
宿主	Rabbit
同种型	IgG
品种	chicken, human, mouse, rat

储存和货运信息
货运代码	Dry Ice Only
毒性	Standard Handling
储存	-20°C
避免冻结 / 解冻	Avoid freeze/thaw
无需冷冻	No
特殊指南	Following initial thaw, aliquot and freeze (-70°C).