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描述 产品目录编号 14-749 品牌系列 Chemicon®
商名 - Chemicon
描述 TAO1 Protein, active, 10 µg 概述 N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. 产品信息 演示 10 μg of enzyme in 100 μL of 50 mM Tris/HCl pH7.5, 300 mM NaCl, 0.1 mM EGTA, 0.03% Brij 35, 270 mM sucrose, 1 mM benzamidine, 0.2 mM PMSF, 0.1% 2-mercaptoethanol. Frozen solution. 应用 应用 Active, N-terminal 6His-tagged, recombinant, human TAO1 amino acids 1-327. For use in Kinase Assays. 主要应用 - Kinase Assay
生物信息 纯度 Purity 93% by SDS PAGE and Coomassie blue staining 来源 Expressed by baculovirus in Sf21 cells. 品种 Human 特异活性 1179 U/mg, where one unit of TAO1 activity is defined as 1 nmol phosphate incorporated into 0.8 mg/mL myelin basic protein per minute at 30°C with a final ATP concentration of 100 μM. Entrez基因编号 - NM_020791.1
基因符号 - hKFC-B
- TAOK1
- MAP3K16
- KIAA1361
- PSK2
- MARKK
- FLJ14314
蛋白靶 TAO1 纯化方法 Ni2+/NTA-agarose 靶子系列 STE UniProt编号 - Q7L7X3
UniProt汇总 FUNCTION: SwissProt: Q7L7X3 # Phosphorylates MKK3 (By similarity). Activates the p38 MAP kinase pathway through the specific activation of the upstream MKK3 kinase.
SIZE: 1001 amino acids; 116070 Da
SUBUNIT: Self-associates. Interacts with MKK3 (By similarity).
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in the testis, and to a lower extent also expressed in brain, placenta, colon and skeletal muscle.
PTM: Autophosphorylated (By similarity).
SIMILARITY: SwissProt: Q7L7X3 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 protein kinase domain.产品使用声明 质量保证 Routinely evaluated by phosphorylation of 0.8 mg/mL myelin basic protein. Purity was assessed by SDS-PAGE and Coomassie blue staining using 3 μg of TAO1. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at -70°C from date of shipment. For maximum recovery of product, centrifuge original vial prior to removing the cap.
Rapidly thaw the vial under cold water and immediately place on ice. Aliquot unused material into pre-chilled microcentrifuge tubes and immediately snap-freeze the vials in liquid nitrogen prior to re-storage at -70°C.包装信息 数量 10 µg
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