- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,09-290,Anti-phospho-AMPK1 (Thr174) Antibody
产品名称：Anti-phospho-AMPK1 (Thr174) Antibody
产品型号：09-290
Anti-phospho-AMPK1 (Thr174) Antibody detects level of phospho-AMPK1 (Thr174) & has been published & validated for use in WB, IH.
merck millipore,默克密理博,09-290,Anti-phospho-AMPK1 (Thr174) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ch, H, R, M, Ca, B IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 09-290 描述 Anti-phospho-AMPK1 (Thr174) Antibody Alternate Names - protein kinase, AMP-activated, alpha 1 catalytic subunit
- 5'-AMP-activated protein kinase, catalytic alpha-1 chain
- AMP -activate kinase alpha 1 subunit
- AMP-activated protein kinase, catalytic, alpha-1
- AMPK alpha 1
- AMPK alpha-1 chain
背景信息 AMP-activated protein kinase (AMPK) is a metabolic and stress-sensing kinase that regulates homeostasis, and a key target for treating Type 2 diabetes and obesity. The Alpha subunit has at least two isoforms (alpha 1 and alpha 2) that differ in their subcellular localization and AMP-dependence. AMPK is phosphorylated by upstream kinases, AMPK Kinase (AMPKK) and LKB1 which results in its activation. Active AMPK regulates metabolism by phosphorylating rate-limiting enzymes in metabolic pathways and controlling gene expression. Phosphorylation of threonine 174 in the activation loop of the alpha subunit is a key determinant of AMPK activity. 产品信息 格式 Affinity Purified 控制 - Lambda phosphatase treated and untreated Hek293 cell lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH7.4) 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-phospho-AMPK1 (Thr174) Antibody detects level of phospho-AMPK1 (Thr174) & has been published & validated for use in WB, IH. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Western Blot (SNAP ID) Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in HEK293 cell lysate (see page 2). 生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to phosphorylated human AMPK1 (Thr174). 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 This antibody recognizes phosphorylated AMPK1 at Thr174. 品种反应性 ChickenHumanRatMouseCanineBovine Species Reactivity Note Reacts with human. Predicted to react with mouse, rat, dog, cow, and chicken based on sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006242
Entrez基因汇总 The protein encoded by this gene belongs to the ser/thr protein kinase family. It is the catalytic subunit of the 5'-prime-AMP-activated protein kinase (AMPK). AMPK is a cellular energy sensor conserved in all eukaryotic cells. The kinase activity of AMPK is activated by the stimuli that increase the cellular AMP/ATP ratio. AMPK regulates the activities of a number of key metabolic enzymes through phosphorylation. It protects cells from stresses that cause ATP depletion by switching off ATP-consuming biosynthetic pathways. Alternatively spliced transcript variants encoding distinct isoforms have been observed. 基因符号 - MGC57364
- AMPK
- AMPK1
- AMPKa1
- EC 2.7.11.1
- MGC33776
修改 - Phosphorylation
纯化方法 Affinity Purfied UniProt编号 - Q13131
UniProt汇总 FUNCTION: Responsible for the regulation of fatty acid synthesis by phosphorylation of acetyl-CoA carboxylase. It also regulates cholesterol synthesis via phosphorylation and inactivation of hormone-sensitive lipase and hydroxymethylglutaryl-CoA reductase. Appears to act as a metabolic stress-sensing protein kinase switching off biosynthetic pathways when cellular ATP levels are depleted and when 5'-AMP rises in response to fuel limitation and/or hypoxia. This is a catalytic subunit.
CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
COFACTOR: Magnesium.
ENZYME REGULATION: Binding of AMP results in allosteric activation, inducing phosphorylation on Thr-183 by STK11 in complex with STE20-related adapter-alpha (STRAD alpha) pseudo kinase and CAB39. Also activated by phosphorylation by CAMKK2 triggered by a rise in intracellular calcium ions, without detectable changes in the AMP/ATP ratio.
SUBUNIT STRUCTURE: Heterotrimer of an alpha catalytic subunit, a beta and a gamma non-catalytic subunits. Interacts with FNIP1 and FNIP2.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. CAMK Ser/Thr protein kinase family. SNF1 subfamily.
Contains 1 protein kinase domain.
SEQUENCE CAUTION: The sequence AAH48980.1 differs from that shown. Reason: Erroneous initiation. Translation N-terminally shortened.产品使用声明 质量保证 Evaluated by Western Blot in Lambda phosphatase treated and untreated HEK293 cell lysate.
Western Blot Analysis: 2 µg/mL of this antibody detected AMPK1 phosphorylated at Thr174 in Lambda phosphatase treated and untreated HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain refrigerated at 2-8°C for up to 12 months. 包装信息 数量 100 µg
上一件merck millipore产品：merck millipore,默克密理博,MABC164,Anti-Arl1 Antibody, clone 11A12.1
下一件merck millipore产品：merck millipore,默克密理博,551600,Quercetin, Dihydrate - CAS 6151-25-3 - Calbiochem
相关产品
merck millipore,默克密理博,UFC503008,Amicon Ultra-0.5 Centrifugal Filter Unit with Ultracel-30 membrane
merck millipore,默克密理博,14-845,PIP5K1γ Protein, active, 10 µg
merck millipore,默克密理博,AB15334,Anti-Nab2 Antibody
merck millipore,默克密理博,MABC164,Anti-Arl1 Antibody, clone 11A12.1
merck millipore,默克密理博,551600,Quercetin, Dihydrate - CAS 6151-25-3 - Calbiochem
merck millipore,默克密理博,05-1244,Anti-O-GlcNAc Antibody, clone 18B10.C7(3)
merck millipore,默克密理博,SF-2355,SSX1; Human, Cyanine 3 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,09-478,Anti-phospho-LKB1 (Ser307) Antibody
