merck millipore,默克密理博,07-579,Anti-REST Antibody

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merck millipore,默克密理博,07-579,Anti-REST Antibody
产品名称：Anti-REST Antibody
产品型号：07-579
Anti-REST Antibody is a rabbit polyclonal antibody for detection of REST also known as RE1-silencing transcription factor, X2 box repressor, neuron restrictive silencer factor & has been validated in More>>

merck millipore,默克密理博,07-579,Anti-REST Antibody

产品介绍

merck millipore,默克密理博,07-579,Anti-REST Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H, M	WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-579
品牌系列	Upstate
商名	Upstate
描述	Anti-REST Antibody
Alternate Names	repressor binding to the X2 box Neural-restrictive silencer factor RE1-silencing transcription factor X2 box repressor neuron restrictive silencer factor
背景信息	NRSF (neuron-restrictive silencer factor, also designated XBR and REST for RE1-silencing factor) is a silencer protein that binds the DNA sequence element NRSE (neuron-restrictive silencer element). The binding of NRSF to the NRSE represses neuronal gene transcription in non-neuronal cells. Although NRSF is most highly expressed in non-neural tissues, it is also expressed in developing neurons and at low levels in the brain. NRSF contains nine zinc finger domains, but also exists as a C-terminally truncated form produced by alternative splicing. This variant, REST4, contains five of the zinc-finger domains and weakly binds DNA, yet is transported to the nucleus. NRSF associates with mSin3 and HDAC in ventricular myocytes, suggesting a role for NRSF outside the nervous system. Down-regulation of NRSF, which normally occurs upon neural differentiation, is necessary for the proper development of certain classes of neurons. NRSF is required to repress neuronal gene expression in vivo, in both extra-neural and undifferentiated neural tissue.

产品信息
格式	Purified
控制	HeLa nuclear extract Included Positive Antigen Control: Catalog # 12-309, HeLa nuclear extract. Add an equal volume of Laemmli reducing sample buffer to 10 μL of extract and boil for 5 minutes to reduce the preparation. Load 20 μg of reduced extract per lane for minigels.
演示	Purified rabbit IgG in buffer containing 0.014 M phosphate buffer, pH 7.6, 0.175 M NaCl, 0.07% sodium azide, and 30% glycerol. Store at -20°C.

应用
应用	Anti-REST Antibody is a rabbit polyclonal antibody for detection of REST also known as RE1-silencing transcription factor, X2 box repressor, neuron restrictive silencer factor & has been validated in WB.
主要应用	Western Blotting
应用说明	Updated information-Rest is glycosylated. From the summary: "Finally, the glycosylation pattern of the REST protein was analysed, moving from the observation that the molecular weight calculated on REST sequence is about 116 kDa but using western blotting this transcription factor appears to have distinct apparent molecular weight (see Table 1.1): this difference could be explained by post-translational modifications of the proteins, like glycosylation. In fact recently, several studies underlined the importance of O-glycosylation in modulating transcriptional silencing, protein phosphorylation, protein degradation by proteasome and protein–protein interactions [Julenius et al., 2005; Zachara and Hart, 2006]. Deglycosilating analysis showed that REST protein in SH-SY5Y and HEK293 cells is Oglycosylated and not N-glycosylated. Moreover, using several combination of deglycosilating enzymes it is possible to hypothesize the presence of Gal-β(1-3)-GalNAc residues on the endogenous REST, while β(1-4)-linked galactose residues may be present on recombinant REST protein expressed in HEK293 cells. However, the O-glycosylation process produces an immense multiplicity of chemical structures and monosaccharides must be sequentially hydrolyzed by a series of exoglycosidase. Further experiments are needed to characterize all the post-translational modification of the transcription factor REST." In particular the authors show that REST migrates at 200kd in SH-SY5Y cells.

生物信息
免疫原品种	GST fusion protein corresponding to residues 801-1097 of human REST (repressor element RE-1 silencing transcription factor). Also known as NRSF (neuronal restricted silencing factor).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes REST, Mr 200 kDa
同种型	IgG
品种反应性	RatHumanMouse
Species Reactivity Note	Human, mouse and rat.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_005612
Entrez基因汇总	This gene encodes a transcriptional repressor which represses neuronal genes in non-neuronal tissues. It is a member of the Kruppel-type zinc finger transcription factor family. It represses transcription by binding a DNA sequence element called the neuron-restrictive silencer element. The protein is also found in undifferentiated neuronal progenitor cells, and it is thought that this repressor may act as a master negative regular of neurogenesis. Alternatively spliced transcript variants have been described; however, their full length nature has not been determined.
基因符号	XBR REST NRSF
纯化方法	Protein A purfied
UniProt编号	Q13127
UniProt汇总	FUNCTION: SwissProt: Q13127 # Transcriptional repressor which binds neuron-restrictive silencer element (NRSE) and represses neuronal gene transcription in non-neuronal cells. Restricts the expression of neuronal genes by associating with two distinct corepressors, mSin3 and CoREST, which in turn recruit histone deacetylase to the promoters of REST-regulated genes. Mediates repression by recruiting the BHC complex at RE1/NRSE sites which acts by deacetylating and demethylating specific sites on histones, thereby acting as a chromatin modifier. SIZE: 1097 amino acids; 121888 Da SUBUNIT: Interacts with SIN3A, SIN3B and RCOR1. SUBCELLULAR LOCATION: Nucleus (Probable). TISSUE SPECIFICITY: Ubiquitous. Expressed at higher levels in the tissues of the lymphocytic compartment, including spleen, thymus, peripheral blood lymphocytes and ovary. SIMILARITY: SwissProt: Q13127 ## Contains 9 C2H2-type zinc fingers.

产品使用声明
质量保证	Routinely evaluated by western blot with HeLa and Raji RIPA lysate. Western Blot Analysis: 0.5-2 μg/mL of this lot detected REST in nuclear extracts from HeLa and Raji cells.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.