merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB1637,Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

  • merck millipore,默克密理博,MAB1637,Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

    产品名称：Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)
    产品型号：MAB1637
    Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB.

    merck millipore,默克密理博,MAB1637,Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

  • 产品介绍
  • merck millipore,默克密理博,MAB1637,Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Sh, Av, B, H, M, Mk, Po, RWB, ELISA, ICC, IHCMAscitesMonoclonal Antibody
    描述
    产品目录编号 MAB1637
    Replaces 04-1049
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)
    Alternate Names
    • tubulin, beta, 4
    • Tubulin beta-III
    • tubulin, beta 3
    • beta 3
    • beta
    背景信息 Tubulin is one of several members of a small family of globular proteins. The most common members of the tubulin family are α-tubulin and β-tubulin, the proteins which makes up microtubules. Each has a molecular weight of approximately 55 kDa. Microtubules are assembled from dimers of α- and β-tubulin. These subunits are slightly acidic with an isoelectric point between 5.2 and 5.8. Tubulin was long thought to be specific to eukaryotes. Recently, however, the prokaryotic cell division protein FtsZ was shown to be evolutionarily related to tubulin.
    产品信息
    格式 Ascites
    控制
    • Positive control: Brain tissue or neurons of any type.
      Negative control: Non neural tissue or Astrocytes.
    演示 Mouse monoclonal IgG1 ascites in buffer containing PBS containing 1% BSA and 15 mM sodium azide.
    应用
    应用 Anti-Tubulin Antibody, beta III isoform, C-terminus, clone TU-20 (Similar to TUJ1) is an antibody against Tubulin for use in ELISA, IC, IH, IH(P), IP & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunohistochemistry:
    On formalin fixed frozen or paraffin embedded tissue sections: 1:20 to 1:100 dilution from a previous lot. High temperature citrate buffer antigen retrieval is recommended with paraffin embedded tissue sections.

    Immunoprecipitation:
    A previous lot of this antibody was used on Immunoprecipitation.

    Immunocytochemistry:
    1:100 to 1:500 dilution from a previous lot was used on fixed cells.

    Immunoblotting:
    1:1000. Antibody will detect a single band at 50 kDa corresponding to class III beta-tubulin.

    ELISA:
    A previous lot of this antibody was used on ELISA.

    Optimal working dilution must be determined by the end user.
    生物信息
    免疫原品种 A synthetic peptide corresponding to amino acids 443-450 (ESESQGPK) of human class III beta-tubulin conjugated to KLH.
    表位 C-terminus
    克隆 TU-20 (Similar to TUJ1)
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 The antibody reacts with the C-terminus of the neuron specific beta III isoform of tubulin. Does not identify beta tubulin found in glial cells.
    同种型 IgG1
    品种反应性 SheepAvianBovineHumanMouseMonkeyPigRat
    Species Reactivity Note Mouse. Expected to cross-react with Avian, Porcine (Pig), Rat, Bovine, Sheep, Human, Monkey. Reactivity with other species has not been confirmed.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_006086.2
    基因符号
    • MC1R
    • TUBB3
    • TUBB4
    • beta-4
    纯化方法 Unpurified
    UniProt编号
    • Q13509
    UniProt汇总 FUNCTION: SwissProt: Q13509 # Tubulin is the major constituent of microtubules. It binds two moles of GTP, one at an exchangeable site on the beta chain and one at a non-exchangeable site on the alpha-chain.
    SIZE: 450 amino acids; 50433 Da
    SUBUNIT: Dimer of alpha and beta chains.
    DOMAIN:SwissProt: Q13509 The highly acidic C-terminal region may bind cations such as calcium.
    SIMILARITY: Belongs to the tubulin family.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on Mouse Brain lysates.

    Western Blot Analysis:
    1:1000 dilution of this lot detected tubulin (βIII) on 10 μg of Mouse Brain lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C in undiluted aliquots from date of receipt.
    Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µL

    merck millipore,默克密理博,MAB1637,Anti-Tubulin Antibody, beta III isoform, CT, clone TU-20 (Similar to TUJ1)

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