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merck millipore,默克密理博,07-2050,Anti-DNMT3A2 Antibody
产品名称：Anti-DNMT3A2 Antibody
产品型号：07-2050
Use Anti-DNMT3A2 Antibody (Rabbit Polyclonal Antibody) validated in ICC, ELISA, WB to detect DNMT3A2 Antibody also known as DNA (cytosine-5)-methyltransferase 3 alpha.
merck millipore,默克密理博,07-2050,Anti-DNMT3A2 Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB, ICC, ELISA Rb Purified Polyclonal Antibody 描述 产品目录编号 07-2050 描述 Anti-DNMT3A2 Antibody Alternate Names - DNA methyltransferase HsaIIIA
- DNA (cytosine-5-)-methyltransferase 3 alpha
- DNA MTase HsaIIIA
- DNA cytosine methyltransferase 3 alpha
- DNA cytosine methyltransferase 3A2
背景信息 CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. DNMT3 is a developmentally regulated DNA methyltransferase. DNMT3A2 is a second isoform of DNMT3 that is expressed from an alternative promoter. DNMT3A2 localizes to euchromatin and is thought to function in de novo methylation, rather than maintenance methylation. Binding of DNMT3L (DNMT3-like protein) to DNMT3A2 promotes a dramatic reorganization of DNMT3A2 subunits and leads to the formation of specific complexes with enhanced DNA methyltransferase activity. DNMT3A2 targets endogenous DNMT3L to embryonic stem cell chromatin and induces regional DNA methylation. 产品信息 格式 Purified 控制 - L6, NIH/3T3, HeLa, or A431 cells.
演示 Purified rabbit polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. 应用 应用 Use Anti-DNMT3A2 Antibody (Rabbit Polyclonal Antibody) validated in ICC, ELISA, WB to detect DNMT3A2 Antibody also known as DNA (cytosine-5)-methyltransferase 3 alpha. 主要应用 - Western Blotting
- Immunocytochemistry
- ELISA
应用说明 ELISA:
This antibody was shown by an outside laboratory to be suitable for ELISA.
Immunocytochemistry:
This antibody detects DMNT3A2 in the nuclei of NIH/3T3, HeLa, and A431 cells.生物信息 免疫原品种 The immunogen was a KLH-conjugated synthetic peptide corresponding to the C-terminal region of human DNMT3A2. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 The antibody recognizes isoform 2 of human DNMT3A. Additional lower molecular weight bands are detected in some samples. 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. Other homologies: Predicted to react with Mouse and Rat based on 100% sequence homology 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_715640
Entrez基因汇总 CpG methylation is an epigenetic modification that is important for embryonic development, imprinting, and X-chromosome inactivation. Studies in mice have demonstrated that DNA methylation is required for mammalian development. This gene encodes a DNA methyltransferase that is thought to function in de novo methylation, rather than maintenance methylation. The protein localizes to the cytoplasm and nucleus and its expression is developmentally regulated. Alternative splicing results in multiple transcript variants encoding different isoforms. [provided by RefSeq].
基因符号 - OTTHUMP00000120006
- DNMT3A2
- Dnmt3a
- EC 2.1.1.37
- M.HsaIIIA
- OTTHUMP00000120005
修改 - Methylation
纯化方法 Protein A chromatography UniProt编号 - Q9Y6K1
UniProt汇总 FUNCTION:Required for genome wide de novo methylation and is essential for development. DNA methylation is coordinated with methylation of histones. Ref.10
Catalytic activity S-adenosyl-L-methionine + DNA = S-adenosyl-L-homocysteine + DNA containing 5-methylcytosine.
SUBUNIT STRUCTURE: Binds the ZNF238 transcriptional repressor. Interacts with SETDB1. Associates with HDAC1 through its ADD-type zinc-finger By similarity. Interacts with DNMT1 and DNMT3B. Interacts with the PRC2/EED-EZH2 complex.
SUBCELLULAR LOCATION: Nucleus. Cytoplasm. Ref.3
TISSUE SPECIFICITY: Highly expressed in fetal tissues, skeletal muscle, heart, peripheral blood mononuclear cells, kidney, and at lower levels in placenta, brain, liver, colon, spleen, small intestine and lung. Ref.7
Sequence similarities Belongs to the C5-methyltransferase family.
Contains 1 ADD-type zinc finger.
Contains 1 PWWP domain.
CAUTION:It is uncertain whether Met-1 or Met-4 is the initiator
产品使用声明 质量保证 Western Blot Analysis: 1:1,000 dilution of this antibody detected DNMT3A2 on 10 µg of L6 lysate. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial to pellet the solution. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 400 µL
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