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merck millipore,默克密理博,05-949,Anti-Histidine Tagged Antibody, clone HIS.H8
产品名称：Anti-Histidine Tagged Antibody, clone HIS.H8
产品型号：05-949
This Anti-Histidine Tagged Antibody, clone HIS.H8 is validated for use in IC, IP, WB for the detection of Histidine Tagged.
merck millipore,默克密理博,05-949,Anti-Histidine Tagged Antibody, clone HIS.H8
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 A WB, ICC M Purified Monoclonal Antibody 描述 产品目录编号 05-949 品牌系列 Upstate
商名 - Upstate
描述 Anti-Histidine Tagged Antibody, clone HIS.H8 背景信息 His-Tag is a series of at least 6 histidine residues used as a tag motif at the N- or C-terminus of recombinant proteins. The relatively small size of this tag vs. other commonly used tags such as GST (30 kDa) makes it less likely to interfere with target protein structure or function. Its small size also makes it more suitable for use under denaturing conditions. His tags are most often used for affinity purification and binding assays. His tags bind to nickel or cobalt ions with high affinity, and this interaction with metal ions is the basis of many his-tag based affinity media. The His Tag epitope is widely used to detect expression of recombinant proteins in bacterial, yeast, insect,and mammalian systems. 产品信息 格式 Purified 控制 - RIPA lysates of transfected HeLa cells.
演示 Purified mouse monoclonal IgG2b in buffer containing PBS, pH 7.4 with 0.05% sodium azide. 应用 应用 This Anti-Histidine Tagged Antibody, clone HIS.H8 is validated for use in IC, IP, WB for the detection of Histidine Tagged. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry:
2 μg/mL of a previous lot of antibody showed positive immunostaining for His tagged Akt1/PKBα in transfected HeLa cells.
Immunoprecipitation:
This antibody has been reported by an independent laboratory to immunoprecipitate His Tagged proteins.生物信息 免疫原品种 6x His synthetic peptide. 克隆 HIS.H8 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Recognizes tag sequence HHHHHH on either the N- or C-terminus of any recombinant fusion protein; no other amino acids surrounding the 6x-His tag sequence are required for the recognition of the 6x-His tag. 同种型 IgG2b 品种反应性 All Species Reactivity Note All Species. 抗体类型 Monoclonal Antibody 纯化方法 Purified 产品使用声明 质量保证 Routinely evaluated by western blot and immunocytochemistry on transfected HeLa cells.
Western Blot Analysis:
0.5-2 μg/mL of this lot detected His tagged Akt1/PKBα in RIPA lysates of transfected HeLa cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µg
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下一件merck millipore产品：merck millipore,默克密理博,07-2050,Anti-DNMT3A2 Antibody
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