merck millipore,默克密理博,AB5416P,Anti-Clock Antibody

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merck millipore,默克密理博,AB5416P,Anti-Clock Antibody
产品名称：Anti-Clock Antibody
产品型号：AB5416P
Anti-Clock Antibody detects level of Clock & has been published & validated for use in ELISA & WB.

merck millipore,默克密理博,AB5416P,Anti-Clock Antibody

产品介绍

merck millipore,默克密理博,AB5416P,Anti-Clock Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M	WB, ELISA	Rb	Affinity Purified	Polyclonal Antibody

描述
产品目录编号	AB5416P
品牌系列	Chemicon®
商名	Chemicon
描述	Anti-Clock Antibody
Alternate Names	KAT13D

产品信息
格式	Affinity Purified
演示	Affinity purified immunoglobulin. Liquid in PBS and 0.1% BSA.

应用
应用	Anti-Clock Antibody detects level of Clock & has been published & validated for use in ELISA & WB.
主要应用	Western Blotting ELISA
应用说明	Western blot: 1-10 μg/mL using ECL. ELISA: 1:10,000-1:100,000 using 50-100ng control peptide per well. Optimal working dilutions must be determined by the end user.

生物信息
免疫原品种	An 18 amino acid peptide mapping downstream of the serine rich region of mouse Clock (Antoch et al. 1997; King et al. 1997).
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes mouse Clock. The immunogen shows no significant sequence homology with dPER or other known proteins. SPECIES REACTIVITIES: The immunogen sequence is 92% conserved in rate and 90% in human. Reactivity with other species has not been confirmed.
品种反应性	Mouse
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_004898.2
Entrez基因汇总	This gene encodes a protein that belongs to the basic helix-loop-helix (bHLH) family of transcription factors. Polymorphisms within the encoded protein have been associated with circadian rhythm sleep disorders. A similar protein in mice is a circadian regulator that acts as a transcription factor and forms a heterodimer with aryl hydrocarbon receptor nuclear translocator-like to activate transcription of mouse period 1.
基因符号	clock CLOCK KAT13D hCLOCK KIAA0334
UniProt编号	O15516
UniProt汇总	FUNCTION: SwissProt: O15516 # BMAL1/2-CLOCK heterodimers activate E-box element (3'- CACGTG-5') transcription of a number of proteins of the circadian clock. Activates transcription of PER1 and PER2. This transcription is inhibited in a feedback loop by PER and CRY proteins. SIZE: 846 amino acids; 95304 Da SUBUNIT: Component of the circadian clock oscillator which includes the CRY proteins, CLOCK or NPAS2, BMAL1 or BMAL2, CSNK1D and/or CSNK1E, TIMELESS and the PER proteins. Efficient DNA binding requires dimerization with another bHLH protein. Heterodimerization with BMAL1 is required for E-box-dependent transactivation, for CLOCK nuclear translocation and degradation, and, for phosphorylation of both CLOCK and BMAL1. Interaction with PER and CRY proteins requires translocation to the nucleus. Interaction of the CLOCK-BMAL1 heterodimer with PER or CRY inhibits transcription activation. Binds weakly ARNT and ARNT2 to form heterodimers which bind poorly to the E-box motif (By similarity). SUBCELLULAR LOCATION: Cytoplasm (By similarity). Nucleus (By similarity). Note=Shuffling between the cytoplasm and the nucleus is under circadian regulation and is BMAL1-dependent. Phosphorylated form located in the nucleus (By similarity). TISSUE SPECIFICITY: Expressed in all tissues examined including spleen, thymus, prostate, testis, ovary, small intestine, colon, leukocytes, heart, brain, placenta, lung, liver, skeletal muscle, kidney and pancreas. Highest levels in testis and skeletal muscle. Low levels in thymus, lung and liver. Expressed in all brain regions with highest levels in cerebellum. Highly expressed in the suprachiasmatic nucleus (SCN). PTM: Phosphorylation is dependent on CLOCK-BMAL1 heterodimer formation (By similarity). SIMILARITY: SwissProt: O15516 ## Contains 1 basic helix-loop-helix (bHLH) domain. & Contains 1 PAC (PAS-associated C-terminal) domain. & Contains 2 PAS (PER-ARNT-SIM) domains. MISCELLANEOUS: CLOCK-BMAL1 double mutations within the PAS domains result in syngernistic desensitization to high levels of CRY on repression of CLOCK-BMAL1 transcriptional activity of PER1 and, disrupt circadian rhythmicity.

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles. During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.

储存和货运信息

存储条件

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

During shipment, small volumes of antibody will occasionally become entrapped in the seal of the product vial. For antibodies with volumes of 200 μL or less, we recommend gently tapping the vial on a hard surface or briefly centrifuging the vial in a tabletop centrifuge to dislodge any liquid in the container's cap.