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merck millipore,默克密理博,07-1570,Anti-monomethyl Histone H4 (Lys20) Antibody
产品名称：Anti-monomethyl Histone H4 (Lys20) Antibody
产品型号：07-1570
Anti-monomethyl Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of monomethyl Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20), H4 histone family membe More>>
merck millipore,默克密理博,07-1570,Anti-monomethyl Histone H4 (Lys20) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ChIP, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 07-1570 描述 Anti-monomethyl Histone H4 (Lys20) Antibody Alternate Names - histone cluster 1, H4a
- H4K20me1
- Histone H4 (mono methyl K20)
- H4 histone family, member A
- histone 1, H4a
- histone 1
- H4a
背景信息 Histones are highly conserved proteins that serve as the structural scaffold for the organization of nuclear DNA into chromatin. Histones modifications regulate DNA transcription, repair, recombination, and replication. The most commonly studied modifications are acetylation, phosphorylation, methylation, and ubiquitination. These modifications can alter local chromatin architecture, or recruit trans-acting factors that recognize specific histone modifications (the "histone code" hypothesis). Methylation of histone H4 at lysine 20 has been implicated in transcriptional activation, gene silencing, heterochromatin formation, mitosis, and DNA repair. Histone H4 is progressively methylated at Lys20 during the G(2), M, and G(1) phases of the cell cycle, and methylation of H4Lys20 is a marker for heterochromatin. In mammals, the female X chromosome is coated by the Xist RNA, which is critical for silencing, and is marked mainly by methylation of H3Lys9, H3Lys27 and H4Lys20. 产品信息 格式 Affinity Purified 控制 - HeLa cell acid extracts.
演示 Purified from serum by affinity chromatography. Supplied at 1 mg/ml in 0.1M Tris-Glycine (pH7.4), 15mM NaCl, 0.05% NaN3. 应用 应用 Anti-monomethyl Histone H4 (Lys20) Antibody is a rabbit polyclonal antibody for detection of monomethyl Histone H4 (Lys20) also known as H4K20me1, Histone H4 (mono methyl K20), H4 histone family member A & has been validated in WB. 主要应用 - Chromatin Immunoprecipitation (ChIP)
- Western Blotting
应用说明 Peptide Blocking Assay:
40 μg of histone H4 peptide containing monomethyl lysine 20 abolished detection of histone H4 by anti-monomethyl-Histone H4 (Lys20) in immunoblot analysis of acid extracts of HeLa cells
生物信息 免疫原品种 KLH-conjugated, linear synthetic peptide corresponding to amino acids 15-24
(AKRHR[me1K]VLRD-C) of human Histone H4.
表位 Monomethyl Lys20 宿主 Rabbit 特异性 Recognizes monomethylated Histone H4
(Lys20), Mr 11 kDa.
品种反应性 Human Species Reactivity Note Human. Based on sequence homology, broad species cross-reactivity is expected 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_003538.3
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H4 family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the large histone gene cluster on chromosome 6. [provided by RefSeq]
基因符号 - HIST2H4
- H4/A
- H4/B
- H4/C
- H4/D
- H4/E
- H4/G
- H4/H
- H4/I
- H4/J
- H4/K
- H4/M
- H4/N
- H4/O
- H4/a
- H4F2
- H4FA
- H4FB
- H4FC
- H4FD
- H4FE
- H4FG
- H4FH
- H4FI
- H4FJ
- H4FK
- H4FM
- H4FN
- H4FO
修改 - Methylation
纯化方法 Affinity chromatography UniProt编号 - P62805
UniProt汇总 FUNCTION: Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA
SUBCELLULAR LOCATION: Nucleus.
PTM: Acetylation at Lys-6, Lys-9, Lys-13 and Lys-17 occurs in coding regions of the genome but not in heterochromatin.
PTM: Citrullination at Arg-4 by PADI4 impairs methylation.
PTM: Monomethylation at Arg-4 by PRMT1 favors acetylation at Lys-9 and Lys-13. Demethylation is performed by JMJD6.
PTM: Monomethylated, dimethylated or trimethylated at Lys-21. Monomethylation is performed by SET8. Trimethylation is performed by SUV420H1 and SUV420H2 and induces gene silencing.
PTM: Ubiquitinated by the CUL4-DDB-RBX1 complex in response to ultraviolet irradiation. This may weaken the interaction between histones and DNA and facilitate DNA accessibility to repair proteins.
PTM: Sumoylated, which is associated with transcriptional repression.
SIMILARITY: Belongs to the histone H4 family.
产品使用声明 质量保证 Routinely evaluated by western blot analysis 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 100 µg
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