merck millipore,默克密理博,07-1415,Anti-mTOR (catalystic domain) Antibody

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merck millipore,默克密理博,07-1415,Anti-mTOR (catalystic domain) Antibody
产品名称：Anti-mTOR (catalystic domain) Antibody
产品型号：07-1415
Detect mTOR (catalystic domain) using this Anti-mTOR (catalystic domain) Antibody validated for use in WB, IP, IH(P).

merck millipore,默克密理博,07-1415,Anti-mTOR (catalystic domain) Antibody

产品介绍

merck millipore,默克密理博,07-1415,Anti-mTOR (catalystic domain) Antibody

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
R, H	WB	Rb	Purified	Polyclonal Antibody

描述
产品目录编号	07-1415
Replaces	04-385
描述	Anti-mTOR (catalystic domain) Antibody
Alternate Names	FK506 binding protein 12-rapamycin associated protein Mammalian target of rapamycin FKBP-rapamycin associated protein
背景信息	mTOR (Mammalian Target of Rapamycin, aka FRAP, RAPT or RAFT) is a large 289 kDa Ser/Thr protein kinase that regulates cell cycle progression, cell growth, protein synthesis, ribosome biogenesis, and autophagy. mTOR is an evolutionarily conserved member of the Phosphoinositol Kinase-related Kinase (PIKK) family whose activity is regulated by phosphorylation on Ser2448 by Akt in response to insulin or muscle activity. Interestingly, mTOR is the central component of two multimeric kinase complexes consisting of mTOR and numerous other mTOR binding proteins. These two multimeric protein complexes are designated mTORC1 and mTORC2. mTORC1 (mTOR Complex 1) consists of at least mTOR, Raptor, and GL (mLST8). mTORC1 is known to play a central role in insulin signaling, which is crucial in maintaining metabolic homeostasis. The complex is activated primarily though the PI3 Kinase/Akt pathway. Upon insulin stimulation, Akt activates mTORC1 by phosphorylating and inhibiting TSC (and possibly other yet discovered targets and/or mTOR itself). This inhibits the upstream small GTPase regulator Rheb (Ras homolog enriched in brain). This inhibits the kinase activity of the mTORC1 complex, thus disabling its ability to phosphorylate its downstream targets such as p70 S6K on Thr389 and 4E-BP1 on Thr229. The other mTOR complex, mTORC2 (mTOR Complex 2), is made up of at least mTOR, Rictor, GbL, Sin1, Protor 1 and 2. mTORC2 affects cell proliferation and survival primarily by phosphorylating the hydrophobic motif of Akt on Ser473, a well-known effecter of the PI3 Kinase pathway. In addition to phosphorylating Akt, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Interestingly, unlike mTORC1, the mTORC2 complex appears to not be inhibited by treatment with rapamycin and for this reason is referred to as the rapamycin-insensitive complex. Defects in both mTOR complexes are associated with a variety of diseases, including cancer and diabetes.

产品信息
格式	Purified
控制	L6 Cell Lysate
演示	Purified rabbit polyclonal IgG in PBS containing 10% glycerol with 0.02% sodium azide added as a preservative.

应用
应用	Detect mTOR (catalystic domain) using this Anti-mTOR (catalystic domain) Antibody validated for use in WB, IP, IH(P).
主要应用	Western Blotting

生物信息
免疫原品种	Recombinant GST fusion protein corresponding to amino acids 1223-1290 of human mTOR.
表位	1223-1290 aa mTOR
浓缩	Please refer to the Certificate of Analysis for the lot-specific concentration.
宿主	Rabbit
特异性	Recognizes mTOR (mammalian target of rapamycin). Tested reactivity includes human and rat..
同种型	IgG
品种反应性	RatHuman
Species Reactivity Note	Reacts with Human and Rat.
抗体类型	Polyclonal Antibody
Entrez基因编号	NM_004958.2
Entrez基因汇总	The protein encoded by this gene belongs to a family of phosphatidylinositol kinase-related kinases. These kinases mediate cellular responses to stresses such as DNA damage and nutrient deprivation. This protein acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. The CDT6 gene is located in an intron of this gene. [provided by RefSeq]
基因符号	RAFT1 FRAP1 RAPT1 FLJ44809 MTOR FRAP mTOR FRAP2
纯化方法	Protein A purfied
UniProt编号	P42345
UniProt汇总	FUNCTION: Acts as the target for the cell-cycle arrest and immunosuppressive effects of the FKBP12-rapamycin complex. Part of the TORC2 complex which plays a critical role in AKT1 Ser-473 phosphorylation, and may modulate the phosphorylation of PKCA and regulate actin cytoskeleton organization. SUBUNIT: Interacts with the FKBP12-rapamycin complex. Binds UBQLN1. Forms part of the mammalian target of rapamycin 2 complex (TORC2) comprised of FRAP1, GBL, PRR5, RICTOR and SIN. TORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Binds directly to PRR5 and RICTOR within the TORC2 complex. TISSUE SPECIFICITY: Expressed in numerous tissues, with highest levels in testis. SIMILARITY: Belongs to the PI3/PI4-kinase family. SIMILARITY: Contains 1 FAT domain. SIMILARITY: Contains 1 FATC domain. SIMILARITY: Contains 7 HEAT repeats. SIMILARITY: Contains 1 PI3K/PI4K domain. SEQUENCE CAUTION: Sequence=AAC39933.1; Type=Frameshift; Positions=956, 999;

产品使用声明
质量保证	Routinely evaluated by Western Blot on L6 lysates. Western Blot Analysis: 1:500 dilution of this lot detected mTOR on 10 μg of L6 lysates.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable at -20ºC in undiluted aliquots for up to one year from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable at -20ºC in undiluted aliquots for up to one year from date of receipt.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.