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merck millipore,默克密理博,HTS098M,ChemiSCREEN™ Membrane Preparation Recombinant Human NMU2 Neuromedin U Receptor
产品名称：ChemiSCREEN™ Membrane Preparation Recombinant Human NMU2 Neuromedin U Receptor
产品型号：HTS098M
Human NMU2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS098M,ChemiSCREEN™ Membrane Preparation Recombinant Human NMU2 Neuromedin U Receptor
- 产品介绍
- merck millipore,默克密理博,HTS098M,ChemiSCREEN™ Membrane Preparation Recombinant Human NMU2 Neuromedin U Receptor
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 NMU2 描述 产品目录编号 HTS098M 商名 - Chemicon
- ChemiScreen
描述 ChemiSCREEN™ Membrane Preparation Recombinant Human NMU2 Neuromedin U Receptor 概述 Human NMU2 背景信息 Neuromedin U (NmU) is a peptide which regulates peripheral functions such as smooth muscle contraction and blood pressure, and CNS functions including nociception and feeding activity (Brighton et al., 2004a). Two GPCRs, NMU1 and NMU2, mediate the contractile effects of neuromedin U by activation of both Gq and Gi (Brighton et al., 2004b). Compared to the wide distribution of NMU1 in peripheral tissue, expression of NMU2 receptor is limited to areas of the brain, such as the paraventricular nucleus, along the wall of the third ventricle in the hypothalamus and the CA1 region of the hippocampus, and to the spinal cord (Howard et al. 2000). Recent study has shown that mice deficient in NMU2 but not NMU1 receptor had impaired nociceptive responses suggesting that the pro-nociceptive effects of NmU in mice appear to be mediated through NMU2 (Zeng et al., 2006; Torres et al. 2007). Millipore's NMU2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of NMU1 interactions with neuromedin U25. The membrane preparations exhibit a Kd of 0.58 nM for [125I]-neuromedin U-25. With 5 μg/well NMU2 Membrane Prep and 0.25 nM [125I]-neuromedin U-25, a greater than 10-fold signal-to-background ratio was obtained. 产品信息 格式 Membranes 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to the indicated concentration in packaging buffer, rapidly frozen, and stored at -80°C.
应用 应用 Human NMU2 GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human Entrez基因编号 - NM_006056
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 NMU2 靶子系列 Neuromedin U 孵化条件 RECOMMENDED ASSAY CONDITIONS: Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, an FC 96-well harvest plate (Millipore cat. # MAHF C1H) is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
Binding buffer: 50 mM Hepes, pH 7.4, 5 mM MgCl2, 1 mM CaCl2, 0.2% BSA, filtered and stored at 4°C
Radioligand: [125I]-neuromedin U-25 (Perkin Elmer # NEX383)
Wash Buffer: 50 mM Hepes, pH 7.4, 500mM NaCl , 0.1% BSA, filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where an unit is the amount of membrane that will yield greater than 10-fold signal:background with 125I-labeled neuromedin U-25 at 0.25 nM.细胞系类型 - Chem-1
产品使用声明 质量保证 Table 1. Signal:background and specific binding values obtained in a competition binding assay with NMU2 membrane prep.
10 µg/well 5 µg/well Signal:Background 22.4 22.5 Specific Binding (cpm) 10666 7780
SPECIFICATIONS: 1 unit = 5 μg membrane preparation
Bmax: 3.92 pmol/mg
Kd: 0.58 nM
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at –70°C. Product is stable for at least 6 months from the date of receipt when stored as directed. Do not freeze and thaw. 包装信息 数量 200 units
上一件merck millipore产品：merck millipore,默克密理博,46-631MAG,MILLIPLEX MAP Phospho CREB (Ser133) Magnetic Bead MAPmate™ - Cell Signaling Multiplex Assay
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