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merck millipore,默克密理博,07-1332,Anti-RelB (NFĸB family member) Antibody
产品名称：Anti-RelB (NFĸB family member) Antibody
产品型号：07-1332
Anti-RelB (NFĸB family member) Antibody detects level of RelB (NFĸB family member) & has been published & validated for use in WB, IC & IF.
merck millipore,默克密理博,07-1332,Anti-RelB (NFĸB family member) Antibody
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H ICC, WB Rb Purified Polyclonal Antibody 描述 产品目录编号 07-1332 Replaces 04-1077 描述 Anti-RelB (NFĸB family member) Antibody Alternate Names - v-rel reticuloendotheliosis viral oncogene homolog B
- reticuloendotheliosis viral oncogene homolog B
- v-rel avian reticuloendotheliosis viral oncogene homolog B (nuclear<br />factor of kappa light polypeptide gene enhancer in B-cells 3)
背景信息 NFĸB (Nuclear Factor kappa B) represents a group of transcription factors that are structurally related and evolutionarily conserved proteins related to the proto-oncogene c-Rel. The five members in mammals includes Rel (cRel), RelA (p65), RelB, NFĸB1 (p50 and its precursor p105), and NFĸB2 (p52 and its precursor p100) NFĸB/Rel proteins exist as homo- or heterodimer complexes.
In a majority of unstimulated cells, the NFĸB transcription factors are bound to the inhibitory IĸB proteins and retained in the cytoplasm in their inactive form. Upon stimulation by multiple inducers, IĸBα is rapidly phosphorylated and degraded. This results in the release of the NFĸB complex to the nucleus.
产品信息 格式 Purified 演示 Purified rabbit polyclonal in PBS with 0.1% NaN3 in 50% glycerol. 应用 应用 Anti-RelB (NFĸB family member) Antibody detects level of RelB (NFĸB family member) & has been published & validated for use in WB, IC & IF. 主要应用 - Immunocytochemistry
- Western Blotting
生物信息 免疫原品种 GST fusion protein corresponding to the C-terminal region of human RelB. 表位 C-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Detects RelB 同种型 IgG 品种反应性 Human Species Reactivity Note Human. Other species have not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_006500.2
基因符号 - IREL
- I-REL
- I-Rel
纯化方法 Immunogen Affinity Purified and anti-GST depleted. UniProt编号 - Q01201
UniProt汇总 Function: NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively. NF-kappa-B is controlled by various mechanisms of post-translational modification and subcellular compartmentalization as well as by interactions with other cofactors or corepressors. NF-kappa-B complexes are held in the cytoplasm in an inactive state complexed with members of the NF-kappa-B inhibitor (I-kappa-B) family. In a conventional activation pathway, I-kappa-B is phosphorylated by I-kappa-B kinases (IKKs) in response to different activators, subsequently degraded thus liberating the active NF-kappa-B complex which translocates to the nucleus. NF-kappa-B heterodimeric RelB-p50 and RelB-p52 complexes are transcriptional activators. RELB neither associates with DNA nor with RELA/p65 or REL. Stimulates promoter activity in the presence of NFKB2/p49. Ref.5 Ref.6 Ref.7
Subunit structure: Component of the NF-kappa-B RelB-p50 complex. Component of the NF-kappa-B RelB-p52 complex. Self-associates; the interaction seems to be transient and may prevent degradation allowing for heterodimer formation with p50 or p52. Interacts with NFKB1/p50, NFKB2/p52 and NFKB2/p100. Interacts with NFKBID By similarity.
Subcellular location: Nucleus.
Induction By: mitogens.
Domain: Both N- and C-terminal domains are required for transcriptional activation.
Post-translational modification: Phosphorylation at 'Thr-103' and 'Ser-573' is followed by proteasomal degradation By similarity.
Sequence similarities: Contains 1 RHD (Rel-like) domain.
Caution: Was originally (Ref.1) thought to inhibit the transcriptional activity of nuclear factor NF-kappa-B.
产品使用声明 质量保证 Routinely evaluated by Western Blot.
Western Blot Analysis:
This lot detected RelB at 1µg/mL (1:500) dilution in HeLa cell lysate resolved via SDS-PAGE and transferred to PVDF.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution.
包装信息 数量 200 µL
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