- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
-
-
merck millipore,默克密理博,FCSC100184,FlowCellect™ Human Mesenchymal Stem Cell Characterization Kit
产品名称：FlowCellect™ Human Mesenchymal Stem Cell Characterization Kit
产品型号：FCSC100184
This FlowCellect Human Mesenchymal Stem Cell Characterization Kit provides a quantitative solution for clearly identifying human MSC cultures using flow cytometry.
merck millipore,默克密理博,FCSC100184,FlowCellect™ Human Mesenchymal Stem Cell Characterization Kit
- 产品介绍
描述 产品目录编号 FCSC100184 商名 - FlowCellect
描述 FlowCellect™ Human Mesenchymal Stem Cell Characterization Kit 概述 Millipore’s FlowCellect™ Human Mesenchymal Stem Cell Characterization Kit provides a quantitative solution for clearly identifying human MSC cultures using flow cytometry. The kit contains four directly conjugated antibodies to phenotypically characterize hMSCs, with three positive expressing markers (CD105, CD90, and CD44), as well as one negative marker, CD19. These antibodies have been evaluated on a variety of different mesenchymal stem cell culture types such as adipocyte derived stem cells (ADSCs), bone marrow-derived hMSCs, and ES-derived hMSCs, ensuring that the kit can clearly monitor and characterize hMSC cultures from various types.
All kit antibodies are optimized for guava® bench top flow cytometers and provide a complete solution for hMSC analysis. However, this kit can be used on any flow cytometer following the same protocol, offering researchers a reliable, quantitative, and fully validated solution to study their MSC cultures. All four directly conjugated antibodies provided in the kit have been carefully evaluated to ensure optimal performance for flow cytometry, alleviating the need for any additional validation of the kit reagents. Also, plate-based stability testing and quality control was performed to confirm the stability of the antibodies over time. After 120 minutes (2 hours) post sample preparation, kit antibodies did not lose activity (< 5-10% of signal strength). So many tests can be performed in a single experiment without the risk of losing antibody activity or performance over time.Alternate Names - Flow Cellect
背景信息 Human mesenchymal stem cells are adult stem cells which have the capacity for multi-lineage differentiation, giving rise to a variety of mesenchymal phenotypes such as osteoblasts (bone), adipocytes (fat), and chondrocytes (cartilage). Human mesenchymal stem cells, or hMSCs, usually reside in the bone marrow stroma, but have also been indicated to be found in liver, spleen, peripheral blood, umbilical cord blood, and other mesenchymal tissues [2,4,6]. Due to their capacity for self-renewal over long periods of time and the ability to differentiate into specialized cells, interest in understanding the biology of MSC cultures has increased, especially for their potential use for therapy of a variety of diseases.
The International Society for Cellular Therapy (ISCT) has defined a list of guidelines which is used and widely accepted to appropriately identify hMSC cultures. The complexity and multi-lineage capacity of these cell types, can limit identification of a given cell type as MSC in nature. As outlined in the ISCT’s position statement, as well as reiterated by numerous resources (Pittenger et al., 1999, Docheva et al., 2008, and Chamberlain et al., 2007), the undifferentiated state of hMSC cultures can be phenotypically identified and characterized by a series of markers specific to hMSCs., specifically, MSC populations must be positive for at least several antigens, such as CD90, CD105, and CD73 [1,2,3,5,6]. Other resources also concluded that CD44, CD71, and Stro-1 are key markers which can be utilized to accurately validate hMSC cultures [2,3,5]. In fact, CD44 has been indicated to be an important receptor since it is involved in cell-matrix interactions which are shown to be expressed on MSCs. CD44 is abundantly found on chondrocyte cell surfaces and it is an important factor in maintaining cartilage homeostasis [3,7]. In addition to positive expression, negative markers have also been identified, producing less than 2% positive expression of these specific antigens. These cells must lack the expression of hematopoietic antigens like CD45 and CD34, monocytes and macrophages as found on CD14 and CD11b, as well as for B cell markers such as CD79 and CD19 [1,3].产品信息 部件 - 5X Assay Buffer: (Part No. CS202124) One bottle containing 55 mL
- 20X Anti-human CD105/FITC, clone 8E11: (Part No. CS208189). One vial containing 500 uL
- 20X Anti-human CD90/PE, clone F15-42-1: (Part No. CS208185). One vial containing 500 uL
- 20X Anti-human CD44/Alexa Fluor® 647, clone F10-44-2: (Part No. CS208200). One vial containing 500 uL
- 20X Anti-human CD19/PECy5, clone HD37: (Part No. CS208201). One vial containing 300 uL
检测方法 Fluorescence 应用 应用 This FlowCellect Human Mesenchymal Stem Cell Characterization Kit provides a quantitative solution for clearly identifying human MSC cultures using flow cytometry. 主要应用 - Flow Cytometry
生物信息 宿主 Human 品种反应性 Human 基因符号 - CD19
- CD105
- CD90
- CD44
产品使用声明 质量保证 Lot to lot in-process testing on all kit components 使用声明 - Alexa Fluor® is a registered trademark of Molecular Probes, Inc.
- Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 4-8°C; 4 Months 包装信息 数量 100 Tests / Kit
上一件merck millipore产品：merck millipore,默克密理博,CBL497F,Anti-Integrin α5 Antibody, clone SAM-1, FITC conjugated
下一件merck millipore产品：merck millipore,默克密理博,207005,Calcineurin Assay Kit, Colorimetric
相关产品
merck millipore,默克密理博,PC711,Anti-CHIP Rabbit pAb
merck millipore,默克密理博,07-1332,Anti-RelB (NFĸB family member) Antibody
merck millipore,默克密理博,AB15794,Anti-Dexras1 Antibody
merck millipore,默克密理博,311204,NG,NGʹ-Dimethyl-L-arginine, Dihydrochloride - Calbiochem
merck millipore,默克密理博,CBL497F,Anti-Integrin α5 Antibody, clone SAM-1, FITC conjugated
merck millipore,默克密理博,207005,Calcineurin Assay Kit, Colorimetric
merck millipore,默克密理博,MABN1039,Anti-SLC22A3 Antibody, clone EPR6630, rabbit monoclonal
merck millipore,默克密理博,539124,Protease Arrest™ Reagent - Calbiochem
merck millipore,默克密理博,SF-1504,FN1; Human, Cyanine 5 RNA Detection Probe,SmartFlare
merck millipore,默克密理博,SCM088,Human iPS Reprogramming Boost Supplement