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Replacement Information Replacement Information 07-1234 is a recommended replacement for part AB5713 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, ELISA Rb Serum Polyclonal Antibody 描述 产品目录编号 07-1234 Replaces PART AB5713 描述 Anti-Notch 2 Antibody, NT Alternate Names - Notch homolog 2 (Drosophila)
- Neurogenic locus notch homolog protein 2 precursor
- Notch (Drosophila) homolog
- Notch 2
背景信息 Notch is a family of single-pass transmembrane receptor proteins that are synthesized in the endoplasmic reticulum as an inactive form and are proteolytically cleaved on an extracellular site by a furin-like convertase (S1 cleavage) in the trans-golgi network after the recognition of the RQRR sequence before it reaches the plasma membrane as heterodimers to yield an active, ligand-accessible form. The Notch family is comprised of 4 members (1-4) whose ligands include the Delta and Jagged family of ligands. These ligands cause proteolysis of Notch to liberate the intracellular domain. Cleavage results in a C-terminal fragment N(TN) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved (S2 cleavage) by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called Notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin-dependent gamma-secretase between gly1743 and val1744 (S3 cleavage) to release the intracellular domain (NICD) from the membrane. That domain translocates to the nucleus and initiates transcription events by binding the DNA binding protein CSL. The notch family members are involved cell differentiation and development. 产品信息 格式 Serum 控制 - NIH/3T3 Cell Lysate
演示 Rabbit polyclonal IgG serum in buffer containing 0.02 M Potassium phosphate buffer with 0.15 M NaCl and 0.1% sodium azide 应用 应用 Anti-Notch 2 Antibody, N-Terminus detects level of Notch 2 & has been published & validated for use in ELISA, WB, IH(P). 主要应用 - Western Blotting
- ELISA
应用说明 ELISA:
1:30,000-1:90,000 dilution from a previous lot was used in a standard sandwich ELISA assay against the peptide immunogen.
Optimal working dilutions must be determined by the end user.
Immunohistochemistry(paraffin):
Representative testing from a previous lot.
Optimal Staining of NOTCH-2 Polyclonal Antibody: Squamous Cell Carcinoma
生物信息 免疫原品种 Synthetic peptide from the N-terminal sequence of the cleaved N intracellular domain (NICD) human Notch 2. 表位 Cleaved N-terminus 宿主 Rabbit 特异性 Notch 2, cleaved N terminal. This epitope is exposed only after gamma secretase cleavage and is not accessible in the uncleaved form. 同种型 IgG 品种反应性 MouseHuman Species Reactivity Note Human and mouse. The antibody is also expected to react with rat due to immunogen sequence homology (14/15). Other species have not been tested. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_024408.2
Entrez基因汇总 This gene encodes a member of the Notch family. Members of this Type 1 transmembrane protein family share structural characteristics including an extracellular domain consisting of multiple epidermal growth factor-like (EGF) repeats, and an intracellular domain consisting of multiple, different domain types. Notch family members play a role in a variety of developmental processes by controlling cell fate decisions. The Notch signaling network is an evolutionarily conserved intercellular signaling pathway which regulates interactions between physically adjacent cells. In Drosophilia, notch interaction with its cell-bound ligands (delta, serrate) establishes an intercellular signaling pathway that plays a key role in development. Homologues of the notch-ligands have also been identified in human, but precise interactions between these ligands and the human notch homologues remain to be determined. This protein is cleaved in the trans-Golgi network, and presented on the cell surface as a heterodimer. This protein functions as a receptor for membrane bound ligands, and may play a role in vascular, renal and hepatic development. 基因符号 - AGS2
- NOTCH2
- hN2
- OTTHUMP00000059536
UniProt编号 - Q04721
UniProt汇总 FUNCTION: SwissProt: Q04721 # Functions as a receptor for membrane-bound ligands Jagged1, Jagged2 and Delta1 to regulate cell-fate determination. Upon ligand activation through the released notch intracellular domain (NICD) it forms a transcriptional activator complex with RBP-J kappa and activates genes of the enhancer of split locus. Affects the implementation of differentiation, proliferation and apoptotic programs (By similarity).
SIZE: 2471 amino acids; 265405 Da
SUBUNIT: Heterodimer of a C-terminal fragment N(TM) and an N- terminal fragment N(EC) which are probably linked by disulfide bonds (By similarity). Interacts with MAML1, MAML2 and MAML3 which act as transcriptional coactivators for NOTCH2.
SUBCELLULAR LOCATION: Cell membrane; Single-pass type I membrane protein. & Notch 2 intracellular domain: Nucleus. Note=Following proteolytical processing NICD is translocated to the nucleus.
TISSUE SPECIFICITY: Expressed in the brain, heart, kidney, lung, skeletal muscle and liver.
PTM: Synthesized in the endoplasmic reticulum as an inactive form which is proteolytically cleaved by a furin-like convertase in the trans-Golgi network before it reaches the plasma membrane to yield an active, ligand-accessible form. Cleavage results in a C- terminal fragment N(TM) and a N-terminal fragment N(EC). Following ligand binding, it is cleaved by TNF-alpha converting enzyme (TACE) to yield a membrane-associated intermediate fragment called notch extracellular truncation (NEXT). This fragment is then cleaved by presenilin dependent gamma-secretase to release a notch-derived peptide containing the intracellular domain (NICD) from the membrane (By similarity). & Phosphorylated (By similarity).
DISEASE: SwissProt: Q04721 # Defects in NOTCH2 are the cause of Alagille syndrome type 2 (ALGS2) [MIM:610205]. Alagille syndrome is an autosomal dominant multisystem disorder defined clinically by hepatic bile duct paucity and cholestasis in association with cardiac, skeletal, and ophthalmologic manifestations. There are characteristic facial features and less frequent clinical involvement of the renal and vascular systems.
SIMILARITY: SwissProt: Q04721 ## Belongs to the NOTCH family. & Contains 6 ANK repeats. & Contains 35 EGF-like domains. & Contains 3 LNR (Lin/Notch) repeats.产品使用声明 质量保证 Routinely evaluated by Western Blot on NIH/3T3 lysates.
Western Blot Analysis: 1:500 dilution of this lot detected cleaved NOTCH2 on 10 μg of NIH/3T3 lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 6 months at -20°C in undiluted aliquots from date of receipt. Avoid repeated freeze/thaw cycles.
Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 200 µL
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下一件merck millipore产品：merck millipore,默克密理博,14-483,PKCγ Protein, active, 10 µg
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