- 当前位置：首页 > >Merck Millipore > 生命科学研究
- 生命科学研究
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H WB, ICC M Ascites Monoclonal Antibody 描述 产品目录编号 05-723 品牌系列 Upstate
商名 - Upstate
描述 Anti-RhoE/Rnd3 Antibody, clone 4 Alternate Names - small GTP binding protein Rho8
- MemB protein
- Rho family GTPase 3
- ras homolog gene family, member E
- member E
背景信息 RhoE, also known as Rnd3, (Rho family GTPase 3) is a small G-protein that is a member of the Rnd family of Rho GTPases, which is a part of the Ras superfamily. The activity of Rnd1, Rnd2, and Rnd3/RhoE are not activated by the traditional conformational change of the GTP binding like other Rho family members. As the name dictates, Rnd proteins were associated with making a cell “round”. Like other small G-proteins, the do bind GTP, but unlike other G-proteins, they do not hydrolyze GTP to GDP. The actions of RhoE are associated with the actin cytoskeletal organization in response to external stimuli. RhoE acts antagonistically to RhoA in inducing loss of stress fiber formation, in part by binding to the RhoA downstream kinase Rho-associated kinase (ROCK1). This prevents ROCK1 activation and inhibits downstream signaling. RhoE does go through the post-translation modification of farnylation which enables it to localize and bind to various internal membranes. RhoE is also known to effect cycle progression. 产品信息 格式 Ascites 控制 - Included Positive Antigen Control: Catalog #12-305, 3T3/A31 cell lysate. Add 2.5 µL of 2-mercaptoethanol/100 µL of lysate and boil for 5 minutes to reduce the preparation. Load 20 µg of reduced lysate per lane for minigels.
演示 Mouse monoclonal ascites IgG in buffer containing 0.05% sodium azide before the addition of glycerol to 30%. Liquid at -20ºC. 应用 应用 Anti-RhoE/Rnd3 Antibody, clone 4 is an antibody against RhoE/Rnd3 for use in IC & WB. 主要应用 - Western Blotting
- Immunocytochemistry
应用说明 Immunocytochemistry:
A 1:50 dilution of this antibody has been reported by an independent laboratory to show positive immunostaining for RhoE/Rnd3 in 3T3/A31 cells fixed with paraformaldehyde and permeabilized by Triton X-100 (Riento, K., 2003).生物信息 免疫原品种 Full length fusion protein corresponding to residues 1-244 of mouse RhoE/Rnd3. Clone 4. 克隆 4 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes RhoE/Rnd3, Mr 29 kDa. Weakly cross-reacts with Rnd1, Mr 27 kDa. 同种型 IgG 品种反应性 MouseHuman Species Reactivity Note Mouse and human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_028810
Entrez基因汇总 Members of the Rho family of Ras-related GTPases, such as ARHE, regulate the organization of the actin cytoskeleton in response to extracellular growth factors. Like Ras (MIM 190020), Rho family members appear to cycle between an inactive GDP-bound form and an active GTP-bound form. Three major regulators of Rho activity have been identified: RhoGDIs, which interact with the GDP-bound Rho proteins to keep them in a resting complex (see MIM 601925); GEFs, which promote GDP/GTP exchange leading to activation of Rho proteins (see MIM 601855); and GAPs, which stimulate GTP hydrolysis and return the activated Rho protein to its inactive form (see MIM 602680) (Nobes et al., 1998 [PubMed 9531558]).[supplied by OMIM 基因符号 - Rnd3
- RND3
- RHOE
- RhoE
- Rho8
- memB
- ARHE
- RHO8
纯化方法 Unpurified UniProt编号 - P61587
UniProt汇总 FUNCTION: SwissProt: P61587 # Binds GTP but lacks intrinsic GTPase activity and is resistant to Rho-specific GTPase-activating proteins.
SIZE: 244 amino acids; 27368 Da
SUBUNIT: Binds ROCK1 (By similarity). Interacts with UBXD5.
SUBCELLULAR LOCATION: Golgi apparatus membrane; Peripheral membrane protein.
TISSUE SPECIFICITY: Ubiquitous.
SIMILARITY: SwissProt: P61587 ## Belongs to the small GTPase superfamily. Rho family.产品使用声明 质量保证 Routinely evaluated by western blot on RIPA lysates from 3T3/A31 cells.
Western Blot Analysis:
A 1:500-1:2000 dilution of this lot detected RhoE/Rnd3 in RIPA lysates from 3T3/A31 cells.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt. For maximum recovery of product, centrifuge the vial prior to removing the cap. 包装信息 数量 200 µL
上一件merck millipore产品：merck millipore,默克密理博,MAB4197-KC,Mouse Anti-Human Topoisomerase IIα (Ki-S1) (KC)
下一件merck millipore产品：merck millipore,默克密理博,MABS145,Anti-phospho-Sin1 (Ser385) Antibody
相关产品
merck millipore,默克密理博,ABS1021,Anti-Ferroportin-1 Antibody
merck millipore,默克密理博,AP189C,Donkey Anti-Rat IgG Antibody, Cy3 conjugate, Species Adsorbed
merck millipore,默克密理博,MAB1273B,Anti-Mitochondria Antibody, clone 113-1, Biotin Conjugate
merck millipore,默克密理博,AB1921,Anti-Integrin α5 Antibody
merck millipore,默克密理博,MAB4197-KC,Mouse Anti-Human Topoisomerase IIα (Ki-S1) (KC)
merck millipore,默克密理博,MABS145,Anti-phospho-Sin1 (Ser385) Antibody
merck millipore,默克密理博,14-786M,PI3 Kinase (p110α/p65α) Murine, 250 µg
merck millipore,默克密理博,MABE287,Anti-Topoisomerase II Antibody, clone SWT3D1
merck millipore,默克密理博,OS03B,Goat Anti-Rabbit IgG Biotin Conjugate
merck millipore,默克密理博,06-1374,Anti-phospho MRE11 (Thr636) Antibody