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- 体外诊断/OEM材料和试剂
- 产品介绍
描述 产品目录编号 MAB4197-KC 品牌系列 Chemicon®
商名 - Chemicon
描述 Mouse Anti-Human Topoisomerase IIα (Ki-S1) (KC) 概述 This product is intended for use by IVD manufacturers. Alternate Names - Ki-S1
背景信息 Topoisomerase II β (M.W 180 kDa) plays important roles in synthesis and transcription of DNA as well as chromosomal segregation during mitosis. It is present ubiquitously in normal cells and is upregulated in tumors and proliferating cells. Topoisomerase II β is also implicated in drug resistance of tumor cells. 产品信息 格式 Purified 演示 Purified immunoglobulin. 应用 主要应用 - Immunohistochemistry
- Flow Cytometry
- Western Blotting
- Immunocytochemistry
应用说明 Western blot: 1-10μg/ml Immunoprecipitaion
Immunocytochemistry: 5-10 μg/ml Immunohistochemistry: 5-10 μg/ml
Flow cytometry
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Nuclear protein preparation from the human cell line U937.50 克隆 KiS1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 In Immunoprecipitation and Western blot experiments the Ki-S1 antibody recognizes a major protein of 170 kD which was identified as the a isoform of topoisomerase II (Boege et al., 1995; Kreipe et al., 1993). In addition, it has been shown that the Ki-S1 antibody recognizes a carboxyterminal a-isoenzyme specific epitope missing in topoisomerase IIb (Boege et al., 1995). In immunohistochemistry the Ki-S1 antibody shows strong nuclear staining only in proliferating cells. The epitope recognized by the antibody is also detectable in paraffin-embedded tissue sections (Kreipe et al., 1993). Accordingly, it has been shown that the expression of topoisomerase IIa is strongly restricted to proliferating cells (Tsutsui et al., 1993). The topoisomerase IIa antigen is preferentially expressed during G 1 , S, G 2 and M phase of the cell cycle, while resting, non-cycling cells (G 0 phase) lack topoisomerase IIa. In addition, constantly proliferating cells (e.g. cell lines) react positively to topoisomerase IIa during the entire cell-cycle. This specificity of Ki-S1 antibody for proliferating cells might make it a useful tool for determination of the proliferative fraction in solid tumors such as mammary carcinomas (Kreipe et al., 1993; Sampson et al., 1992; Camplejohn et al., 1993; Kreipe et al., 1993; Rasbridge et al., 1994) and gangliomas (Wolf et al., 1994). 同种型 IgG2a 品种 M IgG2a 品种反应性 Human 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001058
Entrez基因汇总 This gene encodes a DNA topoisomerase, an enzyme that controls and alters the topologic states of DNA during transcription. This nuclear enzyme is involved in processes such as chromosome condensation, chromatid separation, and the relief of torsional stress that occurs during DNA transcription and replication. It catalyzes the transient breaking and rejoining of two strands of duplex DNA which allows the strands to pass through one another, thus altering the topology of DNA. Two forms of this enzyme exist as likely products of a gene duplication event. The gene encoding this form, alpha, is localized to chromsome 17 and the beta gene is localized to chromosome 3. The gene encoding this enzyme functions as the target for several anticancer agents and a variety of mutations in this gene have been associated with the development of drug resistance. Reduced activity of this enzyme may also play a role in ataxia-telangiectasia. 基因符号 - EC 5.99.1.3
- TOP2A
- TOP2
- TP2A
UniProt编号 - P11388
UniProt汇总 FUNCTION: SwissProt: P11388 # Control of topological states of DNA by transient breakage and subsequent rejoining of DNA strands. Topoisomerase II makes double-strand breaks.
SIZE: 1531 amino acids; 174385 Da
SUBUNIT: Homodimer. Interacts with COPS5.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus, nucleoplasm. Note=Generally located in the nucleoplasm.
PTM: Phosphorylation has no effect on catalytic activity.
SIMILARITY: SwissProt: P11388 ## Belongs to the type II topoisomerase family.
MISCELLANEOUS: Eukaryotic topoisomerase I and II can relax both negative and positive supercoils, whereas prokaryotic enzymes relax only negative supercoils.产品使用声明 使用声明 - Minimum order quantity required to qualify. Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 12 months.Avoid repeat freeze/thaw cycles. 包装信息 数量 Bulk packaging. Please inquire.
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