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品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 MABS145 描述 Anti-phospho-Sin1 (Ser385) Antibody Alternate Names - mSIN1
- Target of rapamycin complex 2 subunit MAPKAP1
- TORC2 subunit MAPKAP1
- Mitogen-activated protein kinase 2-associated protein 1
- Stress-activated map kinase-interacting protein 1
- SAPK-interacting protein 1
背景信息 Sin1 (stress-activated protein kinase (SAPK)-interacting protein 1, MAPKAP1) is an essential component of the Torc2 complex. mTORC2 is comprised of mTOR, a large Ser/Thr protein kinase along with Sin1, GbL (mLST8), Protor1, Protor2, and Rictor. The complex that is activated primarily downstream of PI3 Kinase and is known to affect cell proliferation and survival primarily by phosphorylating Akt on Ser473. Additionally, the mTORC2 complex is also known to effect cytoskeletal organization and migration by exerting its effects through Rac, Rho, and PKC. Sin1 is also known to directly interact without proteins that include Ras, SPAKs, and JNK. Uniprot.org states 6 different isoforms of Sin1, each with its own observed molecular weight. It is suggested to be involved in metabolic signaling. 产品信息 格式 Purified 控制 - IGF untreated and treated HEK293 cell lysate
演示 Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. 应用 应用 Anti-phospho-Sin1 (Ser385) Antibody is an antibody against phospho-Sin1 (Ser385) for use in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Linear peptide corresponding to mouse Sin1 phosphorylated at Ser385. 表位 Phosphorylated Ser385 克隆 11D1.1 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Sin1 phosphorylated at Ser385. 同种型 IgMκ 品种反应性 Human Species Reactivity Note Demonstrated to react with Human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001006618
Entrez基因汇总 This gene encodes a protein that is highly similar to the yeast SIN1 protein, a stress-activated protein kinase. Alternatively spliced transcript variants encoding distinct isoforms have been described. Alternate polyadenylation sites as well as alternate 3' UTRs have been identified for transcripts of this gene. 基因符号 - SIN1
- MAPKAP1
- MIP1
纯化方法 Purified by ion-exchange chromatography UniProt编号 - Q9BPZ7
UniProt汇总 FUNCTION: Subunit of mTORC2, which regulates cell growth and survival in response to hormonal signals. mTORC2 is activated by growth factors, but, in contrast to mTORC1, seems to be nutrient-insensitive. mTORC2 seems to function upstream of Rho GTPases to regulate the actin cytoskeleton, probably by activating one or more Rho-type guanine nucleotide exchange factors. mTORC2 promotes the serum-induced formation of stress-fibers or F-actin. mTORC2 plays a critical role in AKT1 'Ser-473' phosphorylation, which may facilitate the phosphorylation of the activation loop of AKT1 on 'Thr-308' by PDK1 which is a prerequisite for full activation. mTORC2 regulates the phosphorylation of SGK1 at 'Ser-422'. mTORC2 also modulates the phosphorylation of PRKCA on 'Ser-657'. Within mTORC2, MAPKAP1 is required for complex formation and mTORC2 kinase activity. MAPKAP1 inhibits MAP3K2 by preventing its dimerization and autophosphorylation. Inhibits HRAS and KRAS signaling. Enhances osmotic stress-induced phosphorylation of ATF2 and ATF2-mediated transcription.
SUBUNIT STRUCTURE: Interacts with IFNAR2 By similarity. All isoforms except isoform 4 can be incorporated into the mammalian target of rapamycin complex 2 (mTORC2) which contains MTOR, LST8, PRR5, RICTOR and MAPKAP1. Contrary to mTORC1, mTORC2 does not bind to and is not sensitive to FKBP12-rapamycin. Interacts with ATF2, MAP3K2 and MAPK8. Interacts with GTP-bound HRAS and KRAS.
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein. Cytoplasmic vesicle. Nucleus.产品使用声明 质量保证 Evaluated in Western Blot in IGF untreated and treated HEK293 cell lysate.
Western Blot Analysis: 1 µg/mL of this antibody detected in Sin-1in 10 µg IGF untreated and treated HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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