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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB M Purified Monoclonal Antibody 描述 产品目录编号 05-530 Replaces MABE515 品牌系列 Upstate
商名 - Upstate
描述 Anti-Rad51 Antibody, clone 3C10 背景信息 Rad51 is similar to RecA, both biochemically and structurally. It promotes homologous pairing and strand exchange within a regular nucleoprotein filament. In yeast, the Rad51 family of related proteins also includes Rad55, Rad57, and Dmc1. In mammalian cells, seven genes in this family have been identified (HsRAD51, XRCC2, XRCC3, RAD51B/hREC2 and HsDMC1, RAD51C, and RAD51D). Recent studies suggested that Rad51 is involved not only in homologous recombination but also in cell proliferation regulation. 产品信息 格式 Purified 控制 - HeLa cell nuclear extract
演示 0.1M Tris-glycine, pH 7.4, 0.15M NaCl, 0.05% sodium azide before the addition of glycerol to 30% 应用 应用 Detect Rad51 with Anti-Rad51 Antibody, clone 3C10 (Mouse Monoclonal Antibody), that has been shown to work in IP & WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Full length human Rad51 克隆 clone 3C10 宿主 Mouse 特异性 Rad51; slight cross-reactivity with DMC1 同种型 IgG1 品种反应性 RatHumanMouse 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002875
Entrez基因汇总 The protein encoded by this gene is a member of the RAD51 protein family. RAD51 family members are highly similar to bacterial RecA and Saccharomyces cerevisiae Rad51, and are known to be involved in the homologous recombination and repair of DNA. This protein can interact with the ssDNA-binding protein RPA and RAD52, and it is thought to play roles in homologous pairing and strand transfer of DNA. This protein is also found to interact with BRCA1 and BRCA2, which may be important for the cellular response to DNA damage. BRCA2 is shown to regulate both the intracellular localization and DNA-binding ability of this protein. Loss of these controls following BRCA2 inactivation may be a key event leading to genomic instability and tumorigenesis. Two alternatively spliced transcript variants of this gene, which encode distinct proteins, have been reported. Transcript variants utilizing alternative polyA signals exist. 基因符号 - HsRAD51
- RAD51
- RAD51L3
- hRAD51
- HRAD51
- RECA
- RAD51D
- RAD51A
- HsT16930
- BRCC5
- HsRad51
纯化方法 Protein G chromatography UniProt编号 - Q06609
UniProt汇总 FUNCTION: SwissProt: Q06609 # May participate in a common DNA damage response pathway associated with the activation of homologous recombination and double-strand break repair. Binds to single and double stranded DNA and exhibits DNA-dependent ATPase activity. Underwinds duplex DNA and forms helical nucleoprotein filaments.
SIZE: 339 amino acids; 36966 Da
SUBUNIT: Interacts with BRCA1, BRCA2 and either directly or indirectly with p53. Interacts with XRCC3, RAD54L and RAD54B. Part of a complex with RAD51C and RAD51B. Interacts with RAD51AP1. Interacts with CHEK1/CHK1, and this may require prior phosphorylation of CHEK1.
SUBCELLULAR LOCATION: Nucleus. Note=Colocalizes with RAD51AP1 to multiple nuclear foci upon induction of DNA damage.
TISSUE SPECIFICITY: Highly expressed in testis and thymus, followed by small intestine, placenta, colon, pancreas and ovary. Weakly expressed in breast.
PTM: Phosphorylated. Phosphorylation of Thr-309 by CHEK1/CHK1 may enhance association with chromatin at sites of DNA damage and promote DNA repair by homologous recombination.DISEASE:SwissProt: Q06609 # Defects in RAD51 are associated with breast cancer (BC) [MIM:114480].
SIMILARITY: SwissProt: Q06609 ## Belongs to the recA family. RAD51 subfamily. & Contains 1 HhH domain.产品使用声明 质量保证 routinely evaluated by immunoblot on HeLa nuclear lysate 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 2 years at -20°C 包装信息 数量 200 µg
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