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品种反应性 主要应用 宿主 格式 抗体类型 M WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3120 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Angiopoietin-1 Antibody, NT Alternate Names - Ang-1
背景信息 The remodelling of the primitive vasculature in mammals to form the mature cardiovascular system includes a number of factors directing endothelial cell development. Angiopoietin-1 (mouse and human Ang-1; 498aa with 98% identity) is an angiogenic secreted protein that interacts with the endothelial specific Tie-2 receptor. It is primarily expressed in developing endothelial cells. During embryonic dvevelopment, Ang-1 binds and induces tyrosine phosphorylation of Tie-2. Ang-1 appears to play a crucial role in mediating matrix and mesenchyme. It mediates blood vessel maturation and stability. Ang-1 may play a critical role in heart development. Ang-1 deficient mice mimic the phenotype exhibited by animals deficient in Tie-2. A homolog of Ang-1, termed Angiopoietin-2 (mouse and human Ang-2, 496aa with 85% identity) may act as an antagonist for Ang-1 and Tie-2. Ang-1 and Ang-2 have ~60% sequence homology. 产品信息 格式 Affinity Purified 控制 - Fetal heart
演示 Affinity Purified immunoglobulin. Liquid in PBS with 0.1% BSA. 应用 应用 This Anti-Angiopoietin-1 Antibody, N-terminus is validated for use in ELISA, WB for the detection of Angiopoietin-1. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1-10 ug/mL using ECL. Expected size of Ang-1 is ~55kDa.
ELISA: 1:10,000-1:100,000 using 50-100 ng of control peptide per well (Catalog Number AG635).
Immunohistochemistry: Not tested. It is recommended that the antibody be used at 2-20 ug/ml in formaldehyde fixed tissue.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 20 amino acid peptide sequence near the amino-terminus of mouse Ang-1. 表位 N-terminus 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes mouse Angiopoietin-1 (Ang-1). The immunogenic peptide sequence is 90% (18/20 aa) conserved in human Ang-1, 95% I pig and bovine, and has no significant sequence homology with Ang-2. Antibody crossreactivity in other species is not established. 品种反应性 Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001146.3
Entrez基因汇总 Angiopoietins are proteins with important roles in vascular development and angiogenesis. All angiopoietins bind with similar affinity to an endothelial cell-specific tyrosine-protein kinase receptor. The protein encoded by this gene is a secreted glycoprotein that activates the receptor by inducing its tyrosine phosphorylation. It plays a critical role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. The protein also contributes to blood vessel maturation and stability, and may be involved in early development of the heart. 基因符号 - AGPT
- ANGPT1
- ANG1
- ANG-1
- AGP1
- KIAA0003
- Ang1
纯化方法 Immunoaffinity purified UniProt编号 - Q15389
UniProt汇总 FUNCTION: SwissProt: Q15389 # Binds and activates TIE2 receptor by inducing its tyrosine phosphorylation. Implicated in endothelial developmental processes later and distinct from that of VEGF. Appears to play a crucial role in mediating reciprocal interactions between the endothelium and surrounding matrix and mesenchyme. Mediates blood vessel maturation/stability. It may play an important role in the heart early development.
SIZE: 498 amino acids; 57513 Da
SUBCELLULAR LOCATION: Secreted.
PTM: Glycosylated.
SIMILARITY: SwissProt: Q15389 ## Contains 1 fibrinogen C-terminal domain.
MISCELLANEOUS: It may have a potential therapeutic utility since it can be used for specifically targeting tumor vasculature or for promoting angiogenic processes in certain organs such as an ischemic heart.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 50 µg
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