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Replacement Information Replacement Information 05-516 is a recommended replacement for CBL751 重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R WB, ELISA, FC, ICC, IHC M Purified Monoclonal Antibody 描述 产品目录编号 05-516 Replaces CBL751 品牌系列 Upstate
商名 - Upstate
描述 Anti-Ras Antibody, clone RAS10 Alternate Names - v-Ha-ras Harvey rat sarcoma viral oncogene homolog
- GTP- and GDP-binding peptide B
- GTPase Hras
- Ha-Ras1 proto-oncoprotein
- Ras family small GTP binding protein H-Ras
- Transforming protein p2
- c-has/bas p21 protein
- c-ras-Ki-2 activated oncogene
- p19 H-RasIDX protein
- transformation gene: oncogene HAMSV
背景信息 The tumor oncoproteins HRas, KRas, and NRas are rated as Ras protoncogenes. Point mutations within Ras genes are frequently detected in human malignancies and in experimentally induced tumors in animals. 产品信息 格式 Purified 控制 - Included Positive Antigen Control: Catalog # 12-301, non-stimulated A431 cell lysate. Add 2.5 μL of 2-mercaptoethanol per 100 μL of lysate and boil for 5 minutes to reduce the preparation. Use 20 μg per lane for minigels
演示 Purified mouse monoclonal IgG2ak in buffer containing PBS, pH 7.4, with 0.05% sodium azide and 30% glycerol. Liquid at -20°C. 应用 应用 Detect Ras using this Anti-Ras Antibody, clone RAS10 validated for use in ELISA, FC, IC, IH, IP & WB. 主要应用 - Western Blotting
- ELISA
- Flow Cytometry
- Immunocytochemistry
- Immunohistochemistry
应用说明 Immunoprecipitation:
1-4 μg of this antibody has been reported to immunoprecipitate Ras.
Immunocytochemistry:
2.5 μg/mL of this antibody has been reported to show positive immunostaining for Ras in SW480 and Y1 cells.
Immunohistochemistry:
This antibody has been reported to detect Ras in frozen and paraffin embedded breast carcinoma sections.生物信息 免疫原品种 Purified recombinant Ras. Clone RAS10. 克隆 RAS10 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes p21H-, K- and N-Ras. 同种型 IgG2aκ 品种反应性 MouseHumanRat Species Reactivity Note Proven to react with human, mouse and rat. Other species unknown. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005343
Entrez基因汇总 Members of the RAS superfamily of GTP-binding proteins, which includes MRAS, are membrane-anchored, intracellular signal transducers responsible for a variety of normal cellular functions. They are oncogenically activated in a significant fraction of tumors.[supplied by OMIM] 基因符号 - H-RAS
- NRAS
- NRAS1
- ALPS4
- K-Ras
- Ki-Ras
- K-Ras2
- Kras-2
- p21B
- KRAS
- RASK2
- HRAS
- HA_RAS
- N-RAS
纯化方法 Protein G purfied UniProt编号 - P01116
UniProt汇总 FUNCTION: Ras proteins bind GDP/GTP and possess intrinsic GTPase activity.
Enzyme regulation:Alternate between an inactive form bound to GDP and an active form bound to GTP. Activated by a guanine nucleotide-exchange factor (GEF) and inactivated by a GTPase-activating protein (GAP).
SIZE: 189 amino acids; 21,656 Da
SUBUNIT: Interacts with PHLPP (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Lipid-anchor; Cytoplasmic side.
Involvement in disease:
Defects in KRAS are a cause of acute myelogenous leukemia (AML) [MIM:601626]. AML is a malignant disease in which hematopoietic precursors are arrested in an early stage of development.
Defects in KRAS are a cause of juvenile myelomonocytic leukemia (JMML) [MIM:607785]. JMML is a pediatric myelodysplastic syndrome that constitutes approximately 30% of childhood cases of myelodysplastic syndrome (MDS) and 2% of leukemia. It is characterized by leukocytosis with tissue infiltration and in vitro hypersensitivity of myeloid progenitors to granulocyte-macrophage colony stimulating factor.
Defects in KRAS are the cause of Noonan syndrome 3 (NS3) [MIM:609942]. Noonan syndrome (NS) [MIM:163950] is a disorder characterized by dysmorphic facial features, short stature, hypertelorism, cardiac anomalies, deafness, motor delay, and a bleeding diathesis. It is a genetically heterogeneous and relatively common syndrome, with an estimated incidence of 1 in 1000-2500 live births. Rarely, NS is associated with juvenile myelomonocytic leukemia (JMML). NS3 inheritance is autosomal dominant.
Defects in KRAS are a cause of cardiofaciocutaneous syndrome (CFC syndrome) [MIM:115150]; also known as cardio-facio-cutaneous syndrome. CFC syndrome is characterized by a distinctive facial appearance, heart defects and mental retardation. Heart defects include pulmonic stenosis, atrial septal defects and hypertrophic cardiomyopathy. Some affected individuals present with ectodermal abnormalities such as sparse, friable hair, hyperkeratotic skin lesions and a generalized ichthyosis-like condition. Typical facial features are similar to Noonan syndrome. They include high forehead with bitemporal constriction, hypoplastic supraorbital ridges, downslanting palpebral fissures, a depressed nasal bridge, and posteriorly angulated ears with prominent helices. The inheritance of CFC syndrome is autosomal dominant.
KRAS mutations are involved in cancer development.产品使用声明 质量保证 Routinely evaluated by western blot on RIPA lysate from human A431 carcinoma cells, human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 or PC-12 cell lysates.
Western Blot Analysis:
0.05 - 0.5 μg/mL of this lot detected Ras in RIPA lysate from human A431 carcinoma cells. A previous lot detected Ras in human HFF, Jurkat, Raji, mouse 3T3, CTLL, rat L-6 and PC- 12 cell lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt. 包装信息 数量 100 µg
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