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品种反应性 主要应用 宿主 格式 抗体类型 R, H WB, ELISA Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB1594P 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Serotonin Transporter Antibody 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in 100 mM Tris, pH 7.5 with 0.2% BSA and 0.05% sodium azide. 应用 应用 Anti-Serotonin Transporter Antibody is an antibody against Serotonin Transporter for use in ELISA & WB. 主要应用 - Western Blotting
- ELISA
应用说明 Western blot: 1-10 μg/mL
Cell pellets are resuspended in 100 μL lysis buffer (25 mM Tris, pH 7.6, 150 mM NaCl, 1 μg/mL aprotonin, 10 μg/mL leupeptin, 1 mM phenylmethylsulfonylfluoride, 1 mM sodium orthovanadate, 1 mM EDTA, and 1% Triton X-100). Samples were incubated for 1 hour at 4°C and were pelleted at high speed for 10 minutes at 4°C, and 100 μg protein per sample was subjected to 10% sodium dodecyl sulfate-polyacrylamide gel electrophoresis.
ELISA: 1:100,000 primary EIA using immunogen only (50-100 ng SERT peptide (Catalog Number AG297)/well). Not tested on purified protein at the stated dilutions.
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 A 15 amino acid sequence between transmembrane domain 7 and 8 of the rat serotonin transporter. This region is presumably from the extracellular domain. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Serotonin Transporter (SERT). An antibody raised against a similar peptide has been shown to react with a SERT in baculovirus cells expressing recombinant rat and human SERT. Antibody activity may be reduced considerably when detecting endogenous SERT from rat or human cells. SERT has been observed to migrate as 94 kDa (rat platelet), 80 kDa (pulmonary membranes) and 76 kDa (rat brain). This could be due to differences in postranslational membrane modifications (e.g. glycosylation). 品种反应性 RatHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_001045.3
Entrez基因汇总 This gene encodes an integral membrane protein that transports the neurotransmitter serotonin from synaptic spaces into presynaptic neurons. The encoded protein terminates the action of serotonin and recycles it in a sodium-dependent manner. This protein is a target of psychomotor stimulants, such as amphetamines and cocaine, and is a member of the sodium:neurotransmitter symporter family. A repeat length polymorphism in the promoter of this gene has been shown to affect the rate of serotonin uptake and may play a role in sudden infant death syndrome, aggressive behavior in Alzheimer disease patients, and depression-susceptibility in people experiencing emotional trauma. 基因符号 - SERT
- SLC6A4
- 5-HTT
- 5HTT
- HTT
- OCD1
- hSERT
UniProt编号 - P31645
UniProt汇总 FUNCTION: SwissProt: P31645 # Terminates the action of serotonin by its high affinity sodium-dependent reuptake into presynaptic terminals.
SIZE: 630 amino acids; 70325 Da
SUBUNIT: Interacts with TGFB1I1.
SUBCELLULAR LOCATION: Membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P31645 ## Belongs to the sodium:neurotransmitter symporter (SNF) family.
MISCELLANEOUS: This protein is the target of psychomotor stimulants such as amphetamines or cocaine.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at -20°C in undiluted aliquots for up to 12 months. Avoid repeated freeze/thaw cycles. 包装信息 数量 50 µg
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下一件merck millipore产品：merck millipore,默克密理博,69909,pET-30a(+) DNA - Novagen
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