merck millipore,默克密理愽
  • merck millipore,默克密理博,05-205,Anti-Phospholamban Antibody, clone A1

  • merck millipore,默克密理博,05-205,Anti-Phospholamban Antibody, clone A1

    产品名称：Anti-Phospholamban Antibody, clone A1
    产品型号：05-205
    Detect Phospholamban using this Anti-Phospholamban Antibody, clone A1 validated for use in ELISA, IC, IH, IP & WB.

    merck millipore,默克密理博,05-205,Anti-Phospholamban Antibody, clone A1

  • 产品介绍
  • merck millipore,默克密理博,05-205,Anti-Phospholamban Antibody, clone A1

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    B, Ca, M, Po, RWB, ELISA, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-205
    品牌系列 Upstate
    商名
    • Upstate
    描述 Anti-Phospholamban Antibody, clone A1
    Alternate Names
    • cardiac phospholamban
    背景信息 Phospholamban (PLB) is a 52 amino acid phosphoprotein which regulates the calcium pump of cardiac sarcoplasmic reticulum (SR). PLB is an oligomer of five identical subunits each having a cytoplasmic and transmembrane domain. The cytoplasmic domain (residues 1 to 25) contains the phosphorylation sites and is highly basic and readily cleaved by proteases; whereas the transmembrane domain (residues 25 to 52) is mostly hydrophobic, protease resistant and stabilizes the pentamer. Following adrenergic stimulation of cardiac muscle, PLB is phosphorylated at Ser16 and at Thr17 which is correlated with stimulation of calcium transport activity across the SR membrane and relaxation of cardiac fibers.
    产品信息
    格式 Purified
    控制
    • Bovine cardiac preparation.
    演示 Purified mouse monoclonal IgG1 in buffer containing 20 mM Tris-HCI, pH 7.4, 0.15 M NaCl with 0.05% sodium azide. Frozen solution.
    应用
    应用 Detect Phospholamban using this Anti-Phospholamban Antibody, clone A1 validated for use in ELISA, IC, IH, IP & WB.
    主要应用
    • Western Blotting
    • ELISA
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunoprecipitation:
    4 μg of a previous lot immunoprecipitated phospholamban from 500 μg of bovine cardiac preparation

    Immunohistochemistry:
    10 μg/mL of previous lots of this antibody detected phospholamban in frozen rat heart tissue sections, fixed for either 5 minutes with ice cold ethanol:acetic acid [95:5] or for 30 minutes with 4% paraformaldehyde, 4% sucrose in PBS, followed by permeabilization with 0.25% Triton X-100 in PBS. Also reported by an independent laboratory (Slack, J. P., 1997).

    Immunocytochemisty:
    Recommended.
    生物信息
    免疫原品种 Bovine phospholamban purified from bovine cardiac sarcoplasmic reticulum. Clone A1.
    克隆 A1
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 This antibody recognizes and is specific for the four forms of phospholamban: phosphorylated and non-phosphorylated phospholamban and the breakdown protein derivatives from the sarcoplasmic reticulum.
    同种型 IgG1
    品种反应性 BovineCanineMousePigRat
    Species Reactivity Note Canine, bovine, rat, mouse, and porcine.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_002667
    Entrez基因汇总 The protein encoded by this gene is found as a pentamer and is a major substrate for the cAMP-dependent protein kinase in cardiac muscle. The encoded protein is an inhibitor of cardiac muscle sarcoplasmic reticulum Ca(2+)-ATPase in the unphosphorylated state, but inhibition is relieved upon phosphorylation of the protein. The subsequent activation of the Ca(2+) pump leads to enhanced muscle relaxation rates, thereby contributing to the inotropic response elicited in heart by beta-agonists. The encoded protein is a key regulator of cardiac diastolic function. Mutations in this gene are a cause of inherited human dilated cardiomyopathy with refractory congestive heart failure.
    基因符号
    • phospholamban
    • PLN
    • CMD1P
    • PLB
    修改
    • Phosphorylation
    纯化方法 Hydroxylapatite chromatography
    UniProt编号
    • P26678
    UniProt汇总 FUNCTION: SwissProt: P26678 # Phospholamban has been postulated to regulate the activity of the calcium pump of cardiac sarcoplasmic reticulum.
    SIZE: 52 amino acids; 6109 Da
    SUBUNIT: Homopentamer.
    SUBCELLULAR LOCATION: Membrane.
    TISSUE SPECIFICITY: Heart.
    PTM: Phosphorylated in response to beta-adrenergic stimulation.
    DISEASE:SwissProt: P26678 # Defects in PLN are the cause of dilated cardiomyopathy 1P (CMD1P) [MIM:609909]. Cardiomyopathy is a disorder characterized by cardiac dilation and reduced systolic function.
    SIMILARITY: SwissProt: P26678 ## Belongs to the phospholamban family.
    产品使用声明
    质量保证 Routinely evaluated on bovine cardiac preparation (do not boil cardiac preparations).

    Western Blot Analysis: 0.5-2 μg/mL of this lot detected phospholamban in 20 μg bovine cardiac preparation. DO NOT BOIL CARDIAC PREPARATION. (Most, P., 2003)
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable for 1 year at -20°C from date of receipt.

    Handling Recommendations:
    Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 50 µg

    merck millipore,默克密理博,05-205,Anti-Phospholamban Antibody, clone A1

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