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merck millipore,默克密理博,05-1526,Anti-Importin subunit alpha-1 Antibody, clone 8G5
产品名称：Anti-Importin subunit alpha-1 Antibody, clone 8G5
产品型号：05-1526
Anti-Importin subunit alpha-1 Antibody, clone 8G5 is a mouse monoclonal antibody for detection of Importin subunit alpha-1 also known as karyopherin alpha 1, importin alpha 5, importin-alpha-S1 & has More>>
merck millipore,默克密理博,05-1526,Anti-Importin subunit alpha-1 Antibody, clone 8G5
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1526 描述 Anti-Importin subunit alpha-1 Antibody, clone 8G5 Alternate Names - karyopherin alpha 1
- karyopherin alpha 1 (importin alpha 5)
- importin alpha 5
- importin-alpha-S1
- recombination activating gene cohort 2
- Karyopherin subunit alpha-1
- Nucleoprotein interactor 1
- RAG cohort protein 2
背景信息 The importin alpha/beta heterodimer facilitates the translocation of hundreds of proteins across the nuclear envelope. Importin alpha binds to proteins containing a classical nuclear localization signal and links them to importin beta ((KPNB1), a protein which ferries the complex through the nuclear pore complex. Movement through the nuclear pore is mediated by the small GTP binding protein Ran via an energy requiring mechanism. Using nucleotide triphosphates and Ran, the nuclear transport complex moves into the nuclear pore and the importin subunits dissociate. Importin alpha and its passenger protein enter the nucleoplasm while importin beta remains at the pore. 产品信息 格式 Purified 控制 - K562 cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Anti-Importin subunit alpha-1 Antibody, clone 8G5 is a mouse monoclonal antibody for detection of Importin subunit alpha-1 also known as karyopherin alpha 1, importin alpha 5, importin-alpha-S1 & has been validated in WB. 主要应用 - Western Blotting
生物信息 免疫原品种 Recombinant protein corresponding to human Importin subunit alpha-1. 表位 Unknown 克隆 8G5 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Importin subunit alpha-1. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_002255.3
Entrez基因汇总 Recombination activating proteins RAG1 and RAG2 regulate and mediate V(D)J recombination, the process by which genes for immunoglobulins and T-cell receptors are generated. Several other ubiquitously expressed proteins are thought to be recruited in the recombination process. Among these are the genes affected in severe combined immune deficiency and genes involved in ds-DNA break repair. The protein encoded by this gene interacts with RAG1 and may play a role in V(D)J recombination. Two transcript variants, one protein-coding and the other not, have been found for this gene. [provided by RefSeq]. 基因符号 - KPNA1
- RCH2
- NPI-1
- SRP1
- SRP1-beta
- IPOA5
纯化方法 Protein G purfied UniProt编号 - P52294
UniProt汇总 FUNCTION: Functions in nuclear protein import as an adapter protein for nuclear receptor KPNB1. Binds specifically and directly to substrates containing either a simple or bipartite NLS motif. Docking of the importin/substrate complex to the nuclear pore complex (NPC) is mediated by KPNB1 through binding to nucleoporin FxFG repeats and the complex is subsequently translocated through the pore by an energy requiring, Ran-dependent mechanism. At the nucleoplasmic side of the NPC, Ran binds to importin-beta and the three components separate and importin-alpha and -beta are re-exported from the nucleus to the cytoplasm where GTP hydrolysis releases Ran from importin. The directionality of nuclear import is thought to be conferred by an asymmetric distribution of the GTP- and GDP-bound forms of Ran between the cytoplasm and nucleus. In vitro, mediates the nuclear import of human cytomegalovirus UL84 by recognizing a non-classical NLS.
SUBUNIT STRUCTURE: Forms a complex with importin subunit beta-1. Interacts with ANP32E by similarity. Interacts with the nucleoprotein of influenza A viruses. Binds to HCMV (human cytomegalovirus) UL84, HIV-1 Vpr and to ebolavirus VP24.
SUBCELLULAR LOCATION: Cytoplasm. Nucleus.
TISSUE SPECIFICITY: Expressed ubiquitously.
DOMAIN: Consists of an N-terminal hydrophilic region, a hydrophobic central region composed of 10 repeats, and a short hydrophilic C-terminus. The N-terminal hydrophilic region contains the importin beta binding domain (IBB domain), which is sufficient for binding importin beta and essential for nuclear protein import.
The IBB domain is thought to act as an intrasteric autoregulatory sequence by interacting with the internal autoinhibitory NLS. Binding of KPNB1 probably overlaps the internal NLS and contributes to a high affinity for cytoplasmic NLS-containing cargo substrates. After dissociation of the importin/substrate complex in the nucleus the internal autohibitory NLS contributes to a low affinity for nuclear NLS-containing proteins by similarity.
The major and minor NLS binding sites are mainly involved in recognition of simple or bipartite NLS motifs. Structurally located within in a helical surface groove they contain several conserved Trp and Asn residues of the corresponding third helices (H3) of ARM repeats which mainly contribute to binding by similarity.
SEQUENCE SIMILARITIES: Belongs to the importin alpha family.
Contains 10 ARM repeats.
Contains 1 IBB domain.产品使用声明 质量保证 Evaluated by Western Blot in K562 cell lysate.
Western Blot Analysis: 0.01 µg/ml of this antibody detected Importin subunit alpha-1 on 10 µg of K562 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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