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merck millipore,默克密理博,05-1352,Anti-Histone H2B Antibody, clone 5HH2-2A8
产品名称：Anti-Histone H2B Antibody, clone 5HH2-2A8
产品型号：05-1352
Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC.
merck millipore,默克密理博,05-1352,Anti-Histone H2B Antibody, clone 5HH2-2A8
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, M, H ICC, WB M Ascites Monoclonal Antibody 描述 产品目录编号 05-1352 Replaces MABE15 描述 Anti-Histone H2B Antibody, clone 5HH2-2A8 Alternate Names - H2B histone family, member Q
- H2B
- Histone H2B
- histone 2, H2be
- histone cluster 2, H2be
背景信息 Histones are basic nuclear proteins that are responsible for the nucleosome structure of chromatin in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which DNA is wrapped in repeating units, called nucleosomes. Histone H2B is one of the main histone proteins involved in the structure of chromatin in eukaryotic cells. Featuring a main globular domain and a long N terminal tail H2B is involved with the structure of the nucleosomes of the 'beads on a string' structure. 产品信息 格式 Ascites 控制 - HepG2 cell lysate, L6 cell lysate, Mouse Brain Lysate
演示 Mouse monoclonal IgG1κ ascites with 0.05% sodium azide. 应用 应用 Anti-Histone H2B Antibody, clone 5HH2-2A8 is a Mouse Monoclonal Antibody for detection of Histone H2B also known as Histone H2B, histone 2 H2be & has been validated in WB, ICC. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 Immunocytochemistry:
A 1:500 dilution of a representative lot of this antibody detected Histone H2B in A431, HeLa and NIH/3T3 cells.生物信息 免疫原品种 Ovalbumin-conjugated synthetic linear peptide corresponding to the C-terminus of Histone H2B. 表位 C-terminus 克隆 clone 5HH2-2A8 宿主 Mouse 特异性 Antibody recognizes Histone H2B near the C-terminus. 同种型 IgG1κ 品种反应性 RatMouseHuman Species Reactivity Note Demonstrated to react in mouse, human and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - BC067487
Entrez基因汇总 Histones are basic nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber in eukaryotes. Two molecules of each of the four core histones (H2A, H2B, H3, and H4) form an octamer, around which approximately 146 bp of DNA is wrapped in repeating units, called nucleosomes. The linker histone, H1, interacts with linker DNA between nucleosomes and functions in the compaction of chromatin into higher order structures. This gene is intronless and encodes a member of the histone H2B family. Transcripts from this gene lack polyA tails but instead contain a palindromic termination element. This gene is found in the small histone gene cluster on chromosome 6p22-p21.3. [provided by RefSeq] 基因符号 - H2BFQ
- H2B
- GL105
- H2B-GL105
- H2B.1
- H2B.q
- H2B/q
纯化方法 Unpurified UniProt编号 - Q99879
UniProt汇总 FUNCTION: SwissProt: Q8N257 # Core component of nucleosome. Nucleosomes wrap and compact DNA into chromatin, limiting DNA accessibility to the cellular machineries which require DNA as a template. Histones thereby play a central role in transcription regulation, DNA repair, DNA replication and chromosomal stability. DNA accessibility is regulated via a complex set of post-translational modifications of histones, also called histone code, and nucleosome remodeling.
SIZE: 126 amino acids; 13908 Da
SUBUNIT: The nucleosome is a histone octamer containing two molecules each of H2A, H2B, H3 and H4 assembled in one H3-H4 heterotetramer and two H2A-H2B heterodimers. The octamer wraps approximately 147 bp of DNA.
SUBCELLULAR LOCATION: Nucleus.
PTM: Monoubiquitination of Lys-121 by the RNF20/40 complex gives a specific tag for epigenetic transcriptional activation and is also prerequisite for histone H3 'Lys-4' and 'Lys-79' methylation. It also functions cooperatively with the FACT dimer to stimulate elongation by RNA polymerase II. & Phosphorylated on Ser-15 by STK4/MST1 during apoptosis; which facilitates apoptotic chromatin condensation. Also phosphorylated on Ser-15 in response to DNA double strand breaks (DSBs), and in correlation with somatic hypermutation and immunoglobulin class- switch recombination.
SIMILARITY: SwissProt: Q8N257 ## Belongs to the histone H2B family.产品使用声明 质量保证 Evaluated by western blot in HepG2 cell lysate.
Western Blot Analysis:
A 1:1,000-1:2,000 dilution of this antibody detected Histone H2B in 10 µg of HepG2 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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