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merck millipore,默克密理博,05-1139,Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7
产品名称：Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7
产品型号：05-1139
This Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7 is validated for use in WB, IP for the detection of Focal Adhesion Kinase.
merck millipore,默克密理博,05-1139,Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ch, H, M, R WB M Purified Monoclonal Antibody 描述 产品目录编号 05-1139 Replaces 04-591 描述 Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7 Alternate Names - focal adhesion kinase 1
- FADK 1
- PTK2 protein tyrosine kinase 2
- Protein-tyrosine kinase 2
背景信息 FAK is a 125 Kd protein that is recruited as a participant in focal adhesion dynamics between cells and has a role in motility and cell survival. FAK is a highly conserved, non-receptor tyrosine kinase originally identified as a substrate for the oncogene protein tyrosine kinase v-src (Guan, J L and Shalloway, D). This cytosolic kinase has been implicated in diverse cellular roles including cell locomotion, mitogen response and cell survival. FAK is typically located at structures known as focal adhesions, these are multi-protein structures that link the extracellular matrix (ECM) to the cytoplasmic cytoskeleton. Additional components of focal adhesions include actin, filamin, vinculin, talin, paxillin and tensin (Burridge, K, et al). 产品信息 格式 Purified 控制 - HEK293 cell lysate
演示 Purified mouse monoclonal IgG1 in buffer containing 0.05% sodium azide. 应用 应用 This Anti-Focal Adhesion Kinase Antibody, clone BLAb2H7 is validated for use in WB, IP for the detection of Focal Adhesion Kinase. 主要应用 - Western Blotting
应用说明 Immunohistochemistry Analysis: A previous lot detected Focal Adhesion Kinase at 1:100 dilution in mouse cortex tissue. 生物信息 免疫原品种 Recombinant fragment derived from the amino terminal region of chicken FAK, amino acid residues 103 to 553. 表位 Terminal 克隆 BLAb2H7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes Focal Adhesion Kinase 同种型 IgG1 品种反应性 ChickenHumanMouseRat Species Reactivity Note Demonstrated to react with human, mouse, rat and chicken. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_990766.1
基因符号 - pp125FAK
- EC 2.7.10.2
- FADK
- FAK
- FAK1
UniProt编号 - Q05397
UniProt汇总 FUNCTION: Non-receptor protein-tyrosine kinase implicated in signaling pathways involved in cell motility, proliferation and apoptosis. Activated by tyrosine-phosphorylation in response to either integrin clustering induced by cell adhesion or antibody cross-linking, or via G-protein coupled receptor (GPCR) occupancy by ligands such as bombesin or lysophosphatidic acid, or via LDL receptor occupancy. Plays a potential role in oncogenic transformations resulting in increased kinase activity.
CATALYTIC ACTIVITY: ATP + a [protein]-L-tyrosine = ADP + a [protein]-L-tyrosine phosphate.
SUBUNIT STRUCTURE: Interacts with CAS family members and with GIT1, SORBS1 and BCAR3. Interacts with RGNEF and SHB By similarity. Interacts with TGFB1I1 and STEAP4.
SUBCELLULAR LOCATION: Cell junction › focal adhesion. Cell membrane; Peripheral membrane protein; Cytoplasmic side. Note: Constituent of focal adhesions.
TISSUE SPECIFICITY: Expressed in all organs tested, in lymphoid cell lines, but most abundantly in brain.
DOMAIN: The first Pro-rich domain interacts with the SH3 domain of CRK-associated substrate (BCAR1) and CASL By similarity.
The carboxy-terminal region is the site of focal adhesion targeting (FAT) sequence which mediates the localization of FAK1 to focal adhesions.
PTM: Phosphorylated on 6 tyrosine residues upon activation.
SEQUENCE SIMILARITIES: Belongs to the protein kinase superfamily. Tyr protein kinase family. FAK subfamily.
Contains 1 FERM domain.
Contains 1 protein kinase domain.产品使用声明 质量保证 Evaluated by Western Blotting in HEK293 cell lysate.
Western Blotting Analysis: 0.2 µg/mL of this antibody detected Focal Adhesion Kinase in 10 µg of HEK293 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt. Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 包装信息 数量 100 µg
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