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merck millipore,默克密理博,04-857,Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
产品名称：Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
产品型号：04-857
Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 is an antibody against phospho-Src family (Tyr416) for use in WB.
merck millipore,默克密理博,04-857,Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
- 产品介绍
- merck millipore,默克密理博,04-857,Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, Vrt WB Rb Culture Supernatant Monoclonal Antibody 描述 产品目录编号 04-857 Replaces 05-857 品牌系列 Upstate
商名 - Upstate
描述 Anti-phospho-Src family (Tyr416) Antibody, clone 2N8, rabbit monoclonal Alternate Names - KAT13A
背景信息 Src (also known as pp60src) is a non receptor Tyrosine Kinase involved in signal transduction in many biological systems and implicated in the development of human tumors. There are two critical phosphorylation sites of tyrosine on Src, tyrosine 418 and tyrosine 529 (referring to human Src sequence). The tyrosine 418 is located in the catalytic domain and is one of the autophosphorylation sites. Full catalytic activity of Src requires phosphorylation of tyrosine 418. This region of Src is also highly conserved in all of the related Src-family kinases, and thus prior immunoprecipitation may be required to identify which Src family member is being activated. 产品信息 格式 Culture Supernatant 控制 - RIPA cell lysate
演示 Cultured supernantant in 0.05% sodium azide 应用 应用 Anti-phospho-Src family (Tyr416) Antibody, clone 2N8 is an antibody against phospho-Src family (Tyr416) for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot Analysis:
A 1:1000 dilution of this lot detected phospho-c-Src in RIPA lysates from COS cells transfected with active Src (Cat # 21-115) (Figure A).
Immunoprecipitation:
A previous lot detected endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) (Figure B).
生物信息 免疫原品种 KLH-conjugated, synthetic peptide containing the sequence ...NE[pY]TA…, where pY corresponds to phosphotyrosine at position 416 of c-Src 克隆 2N8 宿主 Rabbit 特异性 phospho-c-Src (Tyr416) 同种型 IgG 品种反应性 MouseVertebrates Species Reactivity Note Mouse. Broad species cross-reactivity expected based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_005417
Entrez基因汇总 This gene is highly similar to the v-src gene of Rous sarcoma virus. This proto-oncogene may play a role in the regulation of embryonic development and cell growth. The protein encoded by this gene is a tyrosine-protein kinase whose activity can be inhibited by phosphorylation by c-SRC kinase. Mutations in this gene could be involved in the malignant progression of colon cancer. Two transcript variants encoding the same protein have been found for this gene. 基因符号 - SRC
- pp60c-src
- ASV
- c-SRC
- SRC1
- p60-Src
- c-src
修改 - Phosphorylation
UniProt编号 - P12931
UniProt汇总 SIZE: 536 amino acids; 59835 Da
SUBUNIT: Interacts with DDEF1/ASAP1 via its SH3 domain. Interacts with CCPG1 (By similarity). Interacts with CDCP1, PELP1, TGFB1I1 and TOM1L2.
PTM: Phosphorylated on Tyr-530 by c-Src kinase (CSK). The phosphorylated form is termed pp60c-src. The phosphorylated tail interacts with the SH2 domain thereby repressing kinase activity.
SIMILARITY: SwissProt: P12931 ## Belongs to the protein kinase superfamily. Tyr protein kinase family. SRC subfamily. & Contains 1 protein kinase domain. & Contains 1 SH2 domain. & Contains 1 SH3 domain.产品使用声明 质量保证 Routinely evaluated by immunoblot of endogenous phospho-c-Src immunoprecipitated from NIH/3T3 RIPA lysates with anti-Src, clone GD11 agarose beads (Cat # 16-186) 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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