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merck millipore,默克密理博,04-824,Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal
产品名称：Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal
产品型号：04-824
Detect Estrogen Receptor β using this Anti-Estrogen Receptor β Antibody, clone 68-4 validated for use in WB & IP.
merck millipore,默克密理博,04-824,Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Culture Supernatant Monoclonal Antibody 描述 产品目录编号 04-824 描述 Anti-Estrogen Receptor β Antibody, clone 68-4, rabbit monoclonal Alternate Names - estrogen receptor alpha
- Estradiol receptor
- Nuclear receptor subfamily
- estrogen receptor 1
背景信息 Estrogen receptors (ER) are members of the steroid/thyroid hormone receptor superfamily of ligand-activated transcription factors. Estrogen receptors, including ERα and ERβ, contain DNA binding and ligand binding domains and are critically involved in regulating the normal function of reproductive tissues. They are located in the nucleus, though some estrogen receptors associate with the cell surface membrane and can be rapidly activated by exposure of cells to estrogen. ERα and ERβ have been shown to be differentially activated by various ligands. Receptor-ligand interactions trigger a cascade of events, including dissociation from heat shock proteins, receptor dimerization, phosphorylation and the association of the hormone activated receptor with specific regulatory elements in target genes. Evidence suggests that ERα and ERβ may be regulated by distinct mechanisms even though they share many functional characteristics. 产品信息 格式 Culture Supernatant 控制 - MCF7 cell extracts
演示 Cultured supernatant containing 0.05% sodium azide. 应用 应用 Detect Estrogen Receptor β using this Anti-Estrogen Receptor β Antibody, clone 68-4 validated for use in WB & IP. 主要应用 - Western Blotting
应用说明 Immunoprecipitation:
10 μL of a previous lot immunoprecipitated estrogen receptor β from 500 μg of MCF7 RIPA lysate.生物信息 免疫原品种 KLH-conjugated, synthetic peptide corresponding to amino acids 63-82 of rat and mouse estrogen receptor β and amino acids 55-74 of human estrogen receptor β. 表位 a.a.55-74(H) or 63-82 (R, M) 克隆 68-4 宿主 Rabbit 特异性 Estrogen receptor β, long form, does not detect estrogen receptor alpha. 同种型 IgG 品种反应性 RatHumanMouse Species Reactivity Note Reacts with human. Predicted to cross-react with mouse and rat based on sequence homology. 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_001437
Entrez基因汇总 This gene encodes a member of the family of estrogen receptors and superfamily of nuclear receptor transcription factors. The gene product contains an N-terminal DNA binding domain and C-terminal ligand binding domain and is localized to the nucleus, cytoplasm, and mitochondria. Upon binding to 17beta-estradiol or related ligands, the encoded protein forms homo- or hetero-dimers that interact with specific DNA sequences to activate transcription. Some isoforms dominantly inhibit the activity of other estrogen receptor family members. Several alternatively spliced transcript variants of this gene have been described, but the full-length nature of some of these variants has not been fully characterized. [provided by RefSeq].
基因符号 - OTTHUMP00000017719
- DKFZp686N23123
- ER
- ER-alpha
- ESR
- ESRA
- Era
- NR3A1
- OTTHUMP00000017718
UniProt编号 - P03372
UniProt汇总 FUNCTION: Nuclear hormone receptor. The steroid hormones and their receptors are involved in the regulation of eukaryotic gene expression and affect cellular proliferation and differentiation in target tissues.
SUBUNIT: Interacts with SLC30A9 By similarity. Binds DNA as a homodimer. Can form a heterodimer with ESR2. Interacts with NCOA3, NCOA5 and NCOA6 coactivators, leading to a strong increase of transcription of target genes. Interacts with NCOA7 in a ligand-inducible manner. Interacts with PHB2, PELP1 and UBE1C. Interacts with AKAP13. Interacts with CUEDC2. Interacts with JARID1A. Interacts with SMARD1. Interacts with HEXIM1 and MAP1S. Interacts with PBXIP1. Interaction with MUC1 is stimulated by 7 beta-estradiol (E2) and enhances ERS1-mediated transcription. Interacts with DNTTIP2 and FAM120B. Interacts with isoform 4 of TXNRD1. Interacts with MLL2. Interacts with ATAD2 and this interaction is enhanced by estradiol.
SUBCELLULAR LOCATION: Nucleus.
DOMAIN: Composed of three domains: a modulating N-terminal domain, a DNA-binding domain and a C-terminal steroid-binding domain.
PTM: Phosphorylated by cyclin A/CDK2. Phosphorylation probably enhances transcriptional activity. Ref.8 Ref.10 Ref.12 Ref.15 Ref.32
Glycosylated; contains N-acetylglucosamine, probably O-linked. Ref.13
Polymorphism Genetic variations in ESR1 are correlated with bone mineral density (BMD). Low BMD is a risk factor for osteoporotic fracture. Osteoporosis is characterized by reduced bone mineral density, disrutption of bone microarchitecture, and the alteration of the amount and variety of non-collagenous proteins in bone. Osteoporotic bones are more at risk of fracture.
SIMILARITY: Belongs to the nuclear hormone receptor family. NR3 subfamily.
Contains 1 nuclear receptor DNA-binding domain.产品使用声明 质量保证 Evaluated by western blot on RIPA lysates from MCF-7 cells.
Western Blot Analysis:
A 1:500 to 1:2,000 dilution of this antibody detected estrogen receptor β in RIPA lysates from MCF-7 cells.
使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
包装信息 数量 100 µL
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