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品种反应性 主要应用 宿主 格式 抗体类型 Vrt WB M Purified Monoclonal Antibody 描述 产品目录编号 MAB1083 描述 Anti-GFP Antibody, clone 3F8.2 Alternate Names - Green Fluorescent protein
背景信息 The Green Fluorescent Protein (GFP) from the jellyfish Aequorea victoria is used as a fluorescent indicator for monitoring gene expression in a variety of cellular systems, including living organisms and fixed tissues. Unlike other bioluminescent reporters, GFP fluoresces in the absence of substrates, cofactors, or other intrinsic or extrinsic proteins. Purified GFP is a 27 kDa monomer consisting of 238 amino acids and emits green light (emission maximum at 509 nm) when excited with blue or UV light. 产品信息 格式 Purified 控制 - Western Blot: GFP recombinant protein
演示 Purified mouse immunoglobulin in buffer containing PBS containing 0.05% sodium azide. 应用 应用 Detect GFP using this Anti-GFP Antibody, clone 3F8.2 validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot Analysis:
0.125 μg/mL - 1 μg/mL of this antibody detected GFP in 10 ng of GFP recombinant protein (Cat no. 14-392).生物信息 免疫原品种 Full length GFP protein. 表位 Full Length 克隆 3F8.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 The antibody reacts with the 28 kDa GFP protein. 同种型 IgG1κ 品种反应性 Vertebrates Species Reactivity Note Reacts with most common vertebrate proteins containing the GFP tag. 抗体类型 Monoclonal Antibody 基因符号 - GFP
纯化方法 Protein G purfied UniProt编号 - P42212
UniProt汇总 FUNCTION: Energy-transfer acceptor. Its role is to transduce the blue chemiluminescence of the protein aequorin into green fluorescent light by energy transfer. Fluoresces in vivo upon receiving energy from the Ca(2+)-activated photoprotein aequorin.
SUBUNIT: Monomer.
SPECIFICITY: Photocytes.
PTM: Contains a chromophore consisting of modified amino acid residues. The chromophore is formed by autocatalytic backbone condensation between Ser-65 and Gly-67, and oxidation of Tyr-66 to didehydrotyrosine. Maturation of the chromophore requires nothing other than molecular oxygen.
Biotechnological use Green fluorescent protein has been engineered to produce a vast number of variously colored mutants, fusion proteins, and biosensors. Fluorescent proteins and its mutated allelic forms, blue, cyan and yellow have become a useful and ubiquitous tool for making chimeric proteins, where they function as a fluorescent protein tag. Typically they tolerate N- and C-terminal fusion to a broad variety of proteins. They have been expressed in most known cell types and are used as a noninvasive fluorescent marker in living cells and organisms. They enable a wide range of applications where they have functioned as a cell lineage tracer, reporter of gene expression, or as a measure of protein-protein interactions.
Can also be used as a molecular thermometer, allowing accurate temperature measurements in fluids. The measurement process relies on the detection of the blinking of GFP using fluorescence correlation spectroscopy.
SIMILARITIES: Belongs to the GFP family.
Biophysicochemical properties Absorption:
Exhibits a smaller absorbance peak at 470 nm. The fluorescence emission spectrum peaks at 509 nm with a shoulder at 540 nm.
Abs(max)=395 nm
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Ontologies
产品使用声明 质量保证 Routinely evaluated by Western Blot on GFP recombinant protein. 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 1 year after date of receipt. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. 包装信息 数量 100 µg
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