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merck millipore,默克密理博,04-584,Anti-PAK1 Antibody, rabbit monoclonal, clone EP656Y
产品名称：Anti-PAK1 Antibody, rabbit monoclonal, clone EP656Y
产品型号：04-584
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-PAK1 Antibody, Rabbit, clone EP656Y is val More>>
merck millipore,默克密理博,04-584,Anti-PAK1 Antibody, rabbit monoclonal, clone EP656Y
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 R, H, M ICC, WB Rb Purified Monoclonal Antibody 描述 产品目录编号 04-584 品牌系列 Upstate
商名 - Upstate
描述 Anti-PAK1 Antibody, rabbit monoclonal, clone EP656Y Alternate Names - p21-activated kinase 1
背景信息 p21-activated kinase 1 (PAK1) is a member of a family of serine/threonine protein kinase defined by their interaction with the small GTPases, Cdc42 and Rac (1-2). The PAK proteins can be essentially divided into two categories, groups I (PAK1, PAK2 and PAK3) and group II (PAK4, PAK5 and PAK6), based on their structures (3). PAK1 participates in regulating the actin cytoskeleton, focal adhesion contacts, cell motility, apoptosis and transcription. It also promotes the disassembly of stress fibers (4-5). PAK1 also binds PIX (PAK-binding protein), COOL and adapter protein Nck (5). 产品信息 格式 Purified 演示 Buffer: 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl, 40% Glycerol, 0.01% sodium azide and 0.05% BSA. 应用 应用 Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. This Anti-PAK1 Antibody, Rabbit, clone EP656Y is validated for use in IP, WB, IC, IH(P) for the detection of PAK1. 主要应用 - Immunocytochemistry
- Western Blotting
应用说明 WB: 1:10,000-50,000
IHC: 1: 250-500 (Paraffin-embedded human colon cancer)
ICC: 1:250-500 (Hela cells)
IP: 1:50生物信息 免疫原品种 A synthetic peptide corresponding to residues in the human PAK1 protein. 克隆 EP656Y 宿主 Rabbit 特异性 Recognizes Human, Mouse, Rat PAK1. 品种反应性 RatHumanMouse Species Reactivity Note Positive control: Hela cell lysate 抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002576.3
Entrez基因汇总 PAK proteins are critical effectors that link RhoGTPases to cytoskeleton reorganization and nuclear signaling. PAK proteins, a family of serine/threonine p21-activating kinases, include PAK1, PAK2, PAK3 and PAK4. These proteins serve as targets for the small GTP binding proteins Cdc42 and Rac and have been implicated in a wide range of biological activities. PAK1 regulates cell motility and morphology. Alternative transcripts of this gene have been found, but their full-length natures have not yet been determined. 基因符号 - PAKalpha
- PAK1
- p65-PAK
- MGC130001
- Alpha-PAK
- MGC130000
- PAK-1
纯化方法 Protein A purfied UniProt编号 - Q13153
UniProt汇总 FUNCTION: SwissProt: Q13153 # The activated kinase acts on a variety of targets. Likely to be the GTPase effector that links the Rho-related GTPases to the JNK MAP kinase pathway. Activated by CDC42 and RAC1. Involved in dissolution of stress fibers and reorganization of focal complexes. Involved in regulation of microtubule biogenesis through phosphorylation of TBCB. Activity is inhibited in cells undergoing apoptosis, potentially due to binding of CDC2L1 and CDC2L2.
COFACTOR: Magnesium.
SIZE: 545 amino acids; 60647 Da
SUBUNIT: Homodimer in its autoinhibited state. Active as monomer. Interacts tightly with GTP-bound but not GDP-bound CDC42/P21 and RAC1. Binds to the caspase-cleaved p110 isoform of CDC2L1 and CDC2L2, p110C, but not the full-length proteins. Component of cytoplasmic complexes, which also contain PXN, ARHGEF6 and GIT1.
SUBCELLULAR LOCATION: Cytoplasm. Cell junction, focal adhesion. Note=Recruited to focal adhesions upon activation.
PTM: Autophosphorylated when activated by CDC42/p21 and RAC1.
SIMILARITY: SwissProt: Q13153 ## Belongs to the protein kinase superfamily. STE Ser/Thr protein kinase family. STE20 subfamily. & Contains 1 CRIB domain. & Contains 1 protein kinase domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Store at -20 °C. Stable for 12 months from date of receipt. 包装信息 数量 100 µL
上一件merck millipore产品：merck millipore,默克密理博,239803,Cyclopamine, V. californicum - CAS 4449-51-8 - Calbiochem
下一件merck millipore产品：merck millipore,默克密理博,554717,Reversine - CAS 656820-32-5 - Calbiochem
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