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merck millipore,默克密理博,04-1460,Anti-PRMT5 Antibody, clone 23C7
产品名称：Anti-PRMT5 Antibody, clone 23C7
产品型号：04-1460
Use Anti-PRMT5 Antibody, clone 23C7 (mouse monoclonal antibody) validated in WB to detect PRMT5 also known as 72 kDa ICCln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, More>>
merck millipore,默克密理博,04-1460,Anti-PRMT5 Antibody, clone 23C7
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 H WB M Purified Monoclonal Antibody 描述 产品目录编号 04-1460 Replaces MABE551 描述 Anti-PRMT5 Antibody, clone 23C7 Alternate Names - Shk1 kinase-binding protein 1 homolog
- protein arginine methyltransferase 5
- skb1 (S. pombe) homolog
- 72 kDa ICln-binding protein
- HMT1 hnRNP methyltransferase-like 5
- Jak-binding protein 1
- SKB1 homolog
- SKB1 homolog (S. pombe)
背景信息 Arginine methylation of proteins is mediated by the Protein-Arginine Methyl Transferase (PRMT) family of enzymes, which are important in signal transduction, transcription, RNA transport, and splicing. PRMTs are divided into two types defined by their activity. Type I PRMTs (including PRMT 1, 3, 4, and 6) are characterized by the formation of asymmetric dimethylated arginine residues. Type II PRMTs, which include only PRMT5, are defined by the formation of symmetric dimethylated arginine residues. Substrates for PRMT5 include myelin basic protein (MBP) and the spliceosomal D1 and D3 proteins. PRMT5 (also known as Skb1Hs/JBP1) exists as homooligomeric complexes, which includes a dimer and tetramer. Homooligomerization of PRMT5 has an important role in its ability to methylate the MBP protein. 产品信息 格式 Purified 控制 - K562 cell lysate
演示 Purified mouse monoclonal IgG1κ in buffer containing 0.1 M Tris-Glycine (pH 7.4, 150 mM NaCl) with 0.05% sodium azide. 应用 应用 Use Anti-PRMT5 Antibody, clone 23C7 (mouse monoclonal antibody) validated in WB to detect PRMT5 also known as 72 kDa ICCln-binding protein, HMT1 hnRNP methyltransferase-like 5, Jak-binding protein 1, SKB1 homolog. 主要应用 - Western Blotting
生物信息 免疫原品种 Recombinant protein corresponding to human PRMT5. 克隆 23C7 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 This antibody recognizes PRMT5. 同种型 IgG1κ 品种反应性 Human Species Reactivity Note Demonstrated to react with human. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001034708
基因符号 - SKB1
- PRMT5
- HRMT1L5
- IBP72
- SKB1Hs
- JBP1
纯化方法 Protein G purfied UniProt编号 - O14744
UniProt汇总 FUNCTION: Methylates specific arginine residues in the small nuclear ribonucleoproteins Sm D1 and Sm D3 to monomethylarginine and to symmetrical dimethylarginines (sDMAs). Methylates SUPT5H. Mono- and dimethylates arginine residues of myelin basic protein (MBP) in vitro. Plays a role in the assembly of snRNP core particles. May play a role in cytokine-activated transduction pathways. Negatively regulates cyclin E1 promoter activity and cellular proliferation. May regulate the SUPT5H transcriptional elongation properties. May be part of a pathway that is connected to a chloride current, possibly through cytoskeletal rearrangement. Methylates histone H2A/H4 'Arg-3' during germ cell development. Methylates histone H3 'Arg-8', which may repress transcription.
SIZE: 637 amino acids; 72684 Da
SUBUNIT: Forms, at least, homodimers and homotetramers. Interacts with PRDM1 (By similarity). Component of the methylosome, a 20S complex containing at least pICLn, PRMT1/SKB1 and MEP50. Component of a high molecular weight E2F-pocket protein complex, CERC (cyclin E1 repressor complex). Also interacts with Sm proteins, JAK2, SSTR1 and SUPT5H. Associates with SWI/SNF remodeling complexes containing SMARCA2 and SMARCA4.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Ubiquitous.
PTM: Disulfide bonds and non-covalent association mediate homooligomers formation.
SIMILARITY: Belongs to the protein arginine N-methyltransferase family.产品使用声明 质量保证 Evaluated by Western Blot in K562 cell lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PRMT5 on 10 µg of K562 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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