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重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M WB, ICC, IHC Rb Serum Polyclonal Antibody 描述 产品目录编号 AB5840 Replaces MABS167 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Dab1 Antibody Alternate Names - Disabled homolog 1
背景信息 Dab1 is a protein arising from the mouse gene dab, that is related to the Drosophila gene 'disabled'. Mutations in the mouse dab gene may result in the 'scrambler' and 'yotari' phenotypes. Dab encodes a phosphoprotein that binds non-receptor tyrosine kinases and has been implicated in neuron development. Dab1 is expressed in neuronal populations exposed to reelin, as determined by in situ hybridization to embryonic mouse brain tissue. Dab1 may function as a signaling molecule that regulates positioning in the developing brain. Cloning of human DAB1 and sequence determinations suggests a 96% homology to the mouse sequence. Very low density lipoprotein receptors (VLDLR) and apolipoprotein E receptor-2 (APOER2) both bind Dab1 on their cytoplasmic tails. Dab1 expression is upregulated in knockout mice lacking both Vldlr and Apoer2 genes. 产品信息 格式 Serum 控制 - Brain tissue
演示 Delipidated and defibrinated Serum in 0.02M Potassium Phosphate, 0.15 M Sodium Chloride, pH 7.2 with 0.01% sodium azide. 应用 应用 This Anti-Dab1 Antibody is validated for use in IC, IH, IP, WB for the detection of Dab1. 主要应用 - Western Blotting
- Immunocytochemistry
- Immunohistochemistry
应用说明 Western blotting: 1:1,000-1:5,000
Immunocytochemistry: 1:500-1:1,000
Immunohistochemistry: 1:5,000-1:20,000 on cryofixed tissues
Immunoprecipitation
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Highly purified GST-fusion protein corresponding to residues 400-555 of mouse Dab1. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 On western blots of mouse brain extracts, the antibody recognizes a band migrating at approximately 80 kDa corresponding to Dab1. No cross reactivity to human or mouse Dab2. 品种反应性 Mouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_021080.3
Entrez基因汇总 The laminar organization of multiple neuronal types in the cerebral cortex is required for normal cognitive function. In mice, the disabled-1 gene plays a central role in brain development, directing the migration of cortical neurons past previously formed neurons to reach their proper layer. This gene is similar to disabled-1, and the protein encoded by this gene is thought to be a signal transducer that interacts with protein kinase pathways to regulate neuronal positioning in the developing brain. Alternatively spliced transcript variants of this gene have been reported, but their full length nature has not been determined. 基因符号 - DAB1
纯化方法 Serum, delipidated and defibrinated UniProt编号 - O75553
UniProt汇总 FUNCTION: SwissProt: O75553 # Adapter molecule functioning in neural development. May regulate SIAH1 activity (By similarity).
SIZE: 588 amino acids; 63775 Da
SUBUNIT: Associates with the SH2 domains of SRC, FYN and ABL. Interacts with DAB2IP and SIAH1 (By similarity). Interacts with LRP1.
DOMAIN: SwissProt: O75553 The PID domain specifically binds to the Asn-Pro-Xaa- Tyr(P) motif found in many tyrosine-phosphorylated proteins.
PTM: Phosphorylated on Tyr-198 and Tyr-220 upon reelin induction in embryonic neurons (By similarity). Also phosphorylated on Ser- 524 independently of reelin signaling (By similarity).
SIMILARITY: Contains 1 PID domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µL
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