merck millipore,默克密理愽
  • merck millipore,默克密理博,MAB1973,Anti-Integrin α1 Antibody, clone FB12

  • merck millipore,默克密理博,MAB1973,Anti-Integrin α1 Antibody, clone FB12

    产品名称：Anti-Integrin α1 Antibody, clone FB12
    产品型号：MAB1973
    This Anti-Integrin α1 Antibody, clone FB12 is validated for use in ELISA, FC, IC, IH, IP, FUNC, WB for the detection of Integrin α1.

    merck millipore,默克密理博,MAB1973,Anti-Integrin α1 Antibody, clone FB12

  • 产品介绍
  • merck millipore,默克密理博,MAB1973,Anti-Integrin α1 Antibody, clone FB12

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    HWB, ELISA, FC, ICC, IHCMPurifiedMonoclonal Antibody
    描述
    产品目录编号 MAB1973
    品牌系列 Chemicon®
    商名
    • Chemicon
    描述 Anti-Integrin α1 Antibody, clone FB12
    Alternate Names
    • CD49a
    产品信息
    格式 Purified
    控制
    • Microvascular endothelium
    演示 Purified immunoglobulin in 0.02M PB, pH 7.6, 0.25M NaCl containing 0.1% sodium azide.
    应用
    应用 This Anti-Integrin α1 Antibody, clone FB12 is validated for use in ELISA, FC, IC, IH, IP, FUNC, WB for the detection of Integrin α1.
    主要应用
    • Western Blotting
    • ELISA
    • Flow Cytometry
    • Immunocytochemistry
    • Immunohistochemistry
    应用说明 Immunohistochemistry: 2 μg/mL, with paraformaldehyde fixation.
    Immunohistochemistry: Frozen sections (7 µm) of placental tissue fixed with acetone for 1 hr. Antibody used at 1:10 dilution (Sato et al., 2003).
    Immunocytochemistry: iolated EVT cells firxed with 0.5% PFA @ 4°C for 15 minutes followed by acetone at -20°C for 2 minutes. Antibody was used at 1:10 dilution (Sato et al., 2003).
    Immunoblotting: 2 μg/mL

    Immunoprecipitation: 1 μg/mL

    FACS analysis: 2μg/mL (Sato et al., 2005).

    ELISA: 0.5 μg/mL

    Inhibition of activated human lymphocyte binding to laminin, collagen IV and fibronectin (Fabbri et al., 1996): 1-5 μg/mL

    Optimal working dilutions must be determined by end user.
    生物信息
    克隆 FB12
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 Reacts with the I domain (Val151 - Ala364) of human alpha-1 integrin (CD49a)1. Precipitates proteins of Mr 180 kDa, pI 5.9-6.0 (alpha chain) and Mr 115 kDa, pI 4.5-5.0 (beta chain) from human lymphocyte lysates (Zocchi et al., 1991).
    同种型 IgG1
    品种反应性 Human
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_181501.1
    Entrez基因汇总 This gene encodes the alpha 1 subunit of integrin receptors. This protein heterodimerizes with the beta 1 subunit to form a cell-surface receptor for collagen and laminin. The heterodimeric receptor is involved in cell-cell adhesion and may play a role in inflammation and fibrosis. The alpha 1 subunit contains an inserted (I) von Willebrand factor type I domain which is thought to be involved in collagen binding.
    基因符号
    • VLA-1
    • ITGA1
    • CD49a
    • VLA1
    纯化方法 Protein A purfied
    UniProt编号
    • P56199
    UniProt汇总 FUNCTION: SwissProt: P56199 # Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G- E-R in collagen.
    SIZE: 1179 amino acids; 130848 Da
    SUBUNIT: Heterodimer of an alpha and a beta subunit. Alpha-1 associates with beta-1.
    SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein.
    DOMAIN: SwissProt: P56199 The integrin I-domain (insert) is a VWFA domain. Integrins with I-domains do not undergo protease cleavage.
    SIMILARITY: Belongs to the integrin alpha chain family. & Contains 7 FG-GAP repeats. & Contains 1 VWFA domain.
    产品使用声明
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,MAB1973,Anti-Integrin α1 Antibody, clone FB12

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