merck millipore,默克密理愽
  • merck millipore,默克密理博,05-1152,Anti-Erk1/2 (p44/p42) Antibody, clone MK12

  • merck millipore,默克密理博,05-1152,Anti-Erk1/2 (p44/p42) Antibody, clone MK12

    产品名称：Anti-Erk1/2 (p44/p42) Antibody, clone MK12
    产品型号：05-1152
    Detect Erk1/2 (p44/p42) using this Anti-Erk1/2 (p44/p42) Antibody, clone MK12 validated for use in WB, IP, IH(P) & IC.

    merck millipore,默克密理博,05-1152,Anti-Erk1/2 (p44/p42) Antibody, clone MK12

  • 产品介绍
  • merck millipore,默克密理博,05-1152,Anti-Erk1/2 (p44/p42) Antibody, clone MK12

    Replacement Information
    Replacement Information 05-1152 is a recommended replacement for MAB3054

    重要规格表

    品种反应性 主要应用 宿主 格式 抗体类型
    Ch, H, M, R, Ca, Xn, BICC, WBMPurifiedMonoclonal Antibody
    描述
    产品目录编号 05-1152
    Replaces MAB3054
    描述 Anti-Erk1/2 (p44/p42) Antibody, clone MK12
    Alternate Names
    • Extracellular signal-regulated kinase
    • Mitogen-activated protein kinase
    背景信息 Erk (Extracellular signal-Related Kinase) is a family of two, highly homologous proteins denoted as Erk1 (p44, MAPK3) and Erk2 (p42, MAPK1) that both function in the same pathway. The two proteins are often referred to collectively as Erk1/2 or p44/p42 MAP kinase. The Erk pathway is considered the classical, canonical MAPK (Mitogen-Activated Protein Kinase) signaling pathway. It is an evolutionarily conserved pathway that controls and is a critical regulator of the growth and survival through the promotion of cell proliferation and the prevention of apoptosis. Erk is involved in the control of many fundamental cellular processes including cell proliferation, survival, differentiation, apoptosis, motility and metabolism. Erk is activated by growth factor stimulation of receptor tyrosine kinases (RTKs), GPCR, and/or integrin stimulation. This activates the Ras-Raf-MEK-Erk pathway that results in the phosphorylation/activation of Erk1/2 (p44/p42) on the TxY motif (Thr202/Tyr204 and Thr185/Tyr187 for Erk1 & Erk2, respectively).
    产品信息
    格式 Purified
    控制
    • HeLa cell lysate. For western blots load 5-10 µg Rat Pituitary Lysate.
    演示 Purified mouse monoclonal IgG1 in aqueous buffered solution containing 50% glycerol, BSA and ≤0.09% sodium azide.
    应用
    应用 Detect Erk1/2 (p44/p42) using this Anti-Erk1/2 (p44/p42) Antibody, clone MK12 validated for use in WB, IP, IH(P) & IC.
    主要应用
    • Immunocytochemistry
    • Western Blotting
    应用说明 Immunocytochemistry: A previous lot of this antibody was used in IC.

    Immunoprecipitation: A previous lot of this antibody was used in IP.
    Optimal working dilutions must be determined by end user.

    Immunohistochemistry(paraffin): Representative testing from a previous lot.
    Optimal Staining of ERK1/2 with Citrate pH 6.0 Epitope Retrieval: Human Placenta
    .Erk 1/2 representative staining pattern/morphology in human placenta. Tissue pretreated with citrate pH 6, antigen retrieval. A previous lot of
    this antibody was diluted to 1:100, using IHC-Select® Detection with HRP-DAB.
    生物信息
    免疫原品种 Synthetic peptide corresponding to positions 325-345 of rat ERK1.
    克隆 MK12
    浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration.
    宿主 Mouse
    特异性 ERK1 is the 44 kDa member of the ERK family and shares 85% homology with ERK2. By Western blot, this monoclonal recognizes 44 kDa and 42 kDa proteins from multiple cell lysates including NIH/3T3, RAW264, C2C12, and PC12 as well as tissue samples such as rat pituitary.
    同种型 IgG1
    品种反应性 ChickenHumanMouseRatCanineXenopusBovine
    Species Reactivity Note Human, Mouse, Rat, Canine, Xenopus, Bovine, and Chicken.
    抗体类型 Monoclonal Antibody
    Entrez基因编号
    • NM_001040056.1
    Entrez基因汇总 The protein encoded by this gene is a member of the MAP kinase family. MAP kinases, also known as extracellular signal-regulated kinases (ERKs), act in a signaling cascade that regulates various cellular processes such as proliferation, differentiation, and cell cycle progression in response to a variety of extracellular signals. This kinase is activated by upstream kinases, resulting in its translocation to the nucleus where it phosphorylates nuclear targets. Alternatively spliced transcript variants encoding different protein isoforms have been described. [provided by RefSeq]
    基因符号
    • EC 2.7.11.24
    • MAPK3
    • PRKM3
    • ERK1
    • p44erk1
    • MGC20180
    • p44-ERK1
    • P44ERK1
    • ERK-1
    • p44mapk
    • HUMKER1A
    • ERT2
    • P44MAPK
    • p44-MAPK
    • HS44KDAP
    纯化方法 Purified
    UniProt编号
    • P27361
    UniProt汇总 FUNCTION: Involved in both the initiation and regulation of meiosis, mitosis, and postmitotic functions in differentiated cells by phosphorylating a number of transcription factors such as ELK-1. Phosphorylates EIF4EBP1; required for initiation of translation. Phosphorylates microtubule-associated protein 2 (MAP2). Phosphorylates SPZ1 (By similarity). Phosphorylates heat shock factor protein 4 (HSF4).
    CATALYTIC ACTIVITY: ATP + a protein = ADP + a phosphoprotein.
    COFACTOR: Magnesium (By similarity).
    ENZYME REGULATION: Activated by tyrosine phosphorylation in response to insulin and NGF.
    SUBUNIT: Interacts with MORG1 (By similarity). Binds to HIV-1 Nef. This interaction inhibits its kinase activity. Interacts with HSF4 and NISCH.
    DOMAIN: The TXY motif contains the threonine and tyrosine residues whose phosphorylation activates the MAP kinases.
    PTM: Dually phosphorylated on Thr-202 and Tyr-204, which activates the enzyme.
    SIMILARITY: Belongs to the protein kinase superfamily. CMGC Ser/Thr protein kinase family. MAP kinase subfamily.
    SIMILARITY: Contains 1 protein kinase domain.
    产品使用声明
    质量保证 Routinely evaluated by Western Blot on HeLa lysates.
    Western Blot Analysis: 1:5,000 dilution of this lot detected ERK1/2 (MAPK) in NIH/3T3 cell lysates.
    使用声明
    • Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
    储存和货运信息
    存储条件 Stable at -20°C in undiluted aliquots for up to 1 year from date of receipt.
    Handling Recommendations: Upon first thaw, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
    包装信息
    数量 100 µg

    merck millipore,默克密理博,05-1152,Anti-Erk1/2 (p44/p42) Antibody, clone MK12

上一件merck millipore产品：merck millipore,默克密理博,07-2191,Anti-phospho-CapZIP Antibody (Ser179)
下一件merck millipore产品：merck millipore,默克密理博,03-226,HDAC6 (His-tag) human recombinant


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