merck millipore,默克密理博,04-1102,Anti-CD45 (CT) Antibody, clone EP322Y, rabbit monoclonal

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merck millipore,默克密理博,04-1102,Anti-CD45 (CT) Antibody, clone EP322Y, rabbit monoclonal
产品名称：Anti-CD45 (CT) Antibody, clone EP322Y, rabbit monoclonal
产品型号：04-1102
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect CD45 (C-term) using this Anti-CD45 (C-term) A More>>

merck millipore,默克密理博,04-1102,Anti-CD45 (CT) Antibody, clone EP322Y, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1102,Anti-CD45 (CT) Antibody, clone EP322Y, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
H	FC, WB	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1102
描述	Anti-CD45 (CT) Antibody, clone EP322Y, rabbit monoclonal
Alternate Names	protein tyrosine phosphatase, receptor type, c polypeptide CD45 antigen T200 glycoprotein T200 leukocyte common antigen leukocyte-common antigen protein tyrosine phosphatase, receptor type, C
背景信息	CD45 is a receptor-tyrosine phosphatase required for antigen-induced signaling in T-cells and B-cells; its substrates are unknown. Interestingly, it associates with several Src-family tyrosine kinases, which are inhibited by tyrosine phosphorylation in their c-termini, suggesting a mechanism for signaling by CD45.

产品信息
格式	Unpurified
控制	Jurkat lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Detect CD45 (C-term) using this Anti-CD45 (C-term) Antibody, clone EP322Y, Rabbit validated for use in WB, FC.
主要应用	Flow Cytometry Western Blotting
应用说明	Flow Cytometry Analysis: Flow cytometric analysis of permeabilized Jurkat cells using anti-CD45.

生物信息
免疫原品种	Synthetic peptide corresponding to residues in the C-terminal domain of human CD45.
表位	C-terminus
克隆	EP322Y
宿主	Rabbit
特异性	This antibody recognizes CD45 at and around the C-terminus.
同种型	IgG
品种反应性	Human
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_002829.2
Entrez基因汇总	The protein encoded by this gene is a member of the protein tyrosine phosphatase (PTP) family. PTPs are known to be signaling molecules that regulate a variety of cellular processes including cell growth, differentiation, mitotic cycle, and oncogenic transformation. This PTP contains an extracellular domain, a single transmembrane segment and two tandem intracytoplasmic catalytic domains, and thus belongs to receptor type PTP. This gene is specifically expressed in hematopoietic cells. This PTP has been shown to be an essential regulator of T- and B-cell antigen receptor signaling. It functions through either direct interaction with components of the antigen receptor complexes, or by activating various Src family kinases required for the antigen receptor signaling. This PTP also suppresses JAK kinases, and thus functions as a regulator of cytokine receptor signaling. Four alternatively spliced transcripts variants of this gene, which encode distinct isoforms, have been reported. [provided by RefSeq].
基因符号	glycoprotein B220 CD45 CD45R EC 3.1.3.48 GP180 L-CA LCA LY5 T200
纯化方法	Unpurified
UniProt编号	P08575
UniProt汇总	FUNCTION: Required for T-cell activation through the antigen receptor. The first PTPase domain has enzymatic activity, while the second one seems to affect the substrate specificity of the first one. Upon T-cell activation, recruits an dephosphorylates SKAP1 and FYN. Ref.6 Ref.8 Catalytic activity Protein tyrosine phosphate + H2O = protein tyrosine + phosphate. SUBUNIT STRUCTURE: Binds GANAB and PRKCSH By similarity. Interacts with SKAP1. SUBCELLULAR LOCATION: Membrane; Single-pass type I membrane protein. DOMAIN: The first PTPase domain interacts with SKAP1. PTM: Heavily N- and O-glycosylated. DISEASE: Defects in PTPRC are a cause of severe combined immunodeficiency autosomal recessive T-cell-negative/B-cell-positive/NK-cell-positive (T-B+NK+SCID) [MIM:608971]. SCID refers to a genetically and clinically heterogeneous group of rare congenital disorders characterized by impairment of both humoral and cell-mediated immunity, leukopenia, and low or absent antibody levels. Patients with SCID present in infancy with recurrent, persistent infections by opportunistic organisms. The common characteristic of all types of SCID is absence of T-cell-mediated cellular immunity due to a defect in T-cell development. Ref.14 Genetic variations in PTPRC are involved in multiple sclerosis susceptibility (MS) [MIM:126200]. MS is a neurodegenerative disorder characterized by the gradual accumulation of focal plaques of demyelination particularly in the periventricular areas of the brain. Peripheral nerves are not affected. Onset usually in third or fourth decade with intermittent progression over an extended period. The cause is still uncertain. Ref.13 SEQUENCE SIMILARITY: Belongs to the protein-tyrosine phosphatase family. Receptor class 1/6 subfamily. Contains 2 fibronectin type-III domains. Contains 2 tyrosine-protein phosphatase domains.

产品使用声明
质量保证	Evaluated by Western Blotting on Jurkat cell lysates. Western Blotting Analysis: 1:5,000 - 10,000 dilution of this antibody was used to detect CD45 in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.