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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB9206 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-DARPP-32 Antibody, phosphoThr34 产品信息 格式 Affinity Purified 控制 - Cultured primary neurons, rat brain lysate and human breast adenocarcinoma
演示 Affinity Purified immunoglobulin. Liquid in 10 mM HEPES, pH 7.5, 150 mM NaCl, 100 μg per mL BSA with 50% glycerol. 应用 应用 This Anti-DARPP-32 Antibody, phosphoThr34 is validated for use in WB for the detection of DARPP-32. 主要应用 - Western Blotting
应用说明 Western blot: 1:1,000. The antibody recognizes the ~32 kD DARPP-32 protein phosphorylated at Thr34 protein in rat caudate lysate prepared from caudate slices that had been incubated in the absence and presence of forskolin. The labeling by the antibody to DARPP-32 Thr34 is markedly increased by the forskolin treatment.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 Synthetic peptide from DARPP-32, phosphoThr34. 表位 phosphoThr34 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes DARPP-32 phosphorylated at threonine 34 . 品种反应性 RatHumanMouse 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_181505.2
- NM_032192.2
Entrez基因汇总 Midbrain dopaminergic neurons play a critical role in multiple brain functions, and abnormal signaling through dopaminergic pathways has been implicated in several major neurologic and psychiatric disorders. One well-studied target for the actions of dopamine is DARPP32. In the densely dopamine- and glutamate-innervated rat caudate-putamen, DARPP32 is expressed in medium-sized spiny neurons (Ouimet and Greengard, 1990 [PubMed 2191086]) that also express dopamine D1 receptors (Walaas and Greengard, 1984 [PubMed 6319627]). The function of DARPP32 seems to be regulated by receptor stimulation. Both dopaminergic and glutamatergic (NMDA) receptor stimulation regulate the extent of DARPP32 phosphorylation, but in opposite directions (Halpain et al., 1990 [PubMed 2153935]). Dopamine D1 receptor stimulation enhances cAMP formation, resulting in the phosphorylation of DARPP32 (Walaas and Greengard, 1984 [PubMed 6319627]); phosphorylated DARPP32 is a potent protein phosphatase-1 (see MIM 176875) inhibitor (Hemmings et al., 1984 [PubMed 6087160]). NMDA receptor stimulation elevates intracellular calcium, which leads to activation of calcineurin and dephosphorylation of phospho-DARPP32, thereby reducing the phosphatase-1 inhibitory activity of DARPP32 (Halpain et al., 1990 [PubMed 2153935]).[supplied by OMIM] 基因符号 - FLJ20940
- PPP1R1B
- DARPP32
- DARPP-32
修改 - Phosphorylation
纯化方法 Immunoaffinity purified UniProt编号 - Q9UD71
UniProt汇总 FUNCTION: SwissProt: Q9UD71 # Inhibitor of protein-phosphatase 1.
SIZE: 204 amino acids; 22963 Da
SUBCELLULAR LOCATION: Cytoplasm.
PTM: Dopamine- and cyclic AMP-regulated neuronal phosphoprotein. & Phosphorylation of Thr-34 is required for activity (By similarity).
SIMILARITY: SwissProt: Q9UD71 ## Belongs to the protein phosphatase inhibitor 1 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at -20°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µL
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