merck millipore,默克密理博,04-1088,Anti-Caspase-3 (Pro) Antibody, clone E83-103, rabbit monoclonal

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merck millipore,默克密理博,04-1088,Anti-Caspase-3 (Pro) Antibody, clone E83-103, rabbit monoclonal
产品名称：Anti-Caspase-3 (Pro) Antibody, clone E83-103, rabbit monoclonal
产品型号：04-1088
Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Caspase-3 (Pro) Antibody, clone E83-103, Rabbit More>>

merck millipore,默克密理博,04-1088,Anti-Caspase-3 (Pro) Antibody, clone E83-103, rabbit monoclonal

产品介绍

merck millipore,默克密理博,04-1088,Anti-Caspase-3 (Pro) Antibody, clone E83-103, rabbit monoclonal

重要规格表

品种反应性	主要应用	宿主	格式	抗体类型
M, H	FC, WB, ICC	Rb	Unpurified	Monoclonal Antibody

描述
产品目录编号	04-1088
描述	Anti-Caspase-3 (Pro) Antibody, clone E83-103, rabbit monoclonal
Alternate Names	caspase 3, apoptosis-related cysteine protease Cysteine protease CPP32 PARP cleavage protease SREBP cleavage activity 1 Yama protein caspase 3 caspase 3, apoptosis-related cysteine peptidase
背景信息	Caspase-3 (CPP32, Apopain) is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (32 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF.

产品信息
格式	Unpurified
控制	Jurkat lysates
演示	Rabbit Monoclonal in buffer containing 50 mM Tris-Glycine (pH 7.4), 0.15 M NaCl containing 40% Glycerol, 0.01% sodium azide and 0.05% BSA.

应用
应用	Please note that this product will not be available for sale after March 15, 2015. Please select one of the other antibodies against this target. Anti-Caspase-3 (Pro) Antibody, clone E83-103, Rabbit is an antibody against Caspase-3 (Pro) for use in WB, IP, IH(P), IC, FC.
主要应用	Flow Cytometry Western Blotting Immunocytochemistry
应用说明	Immunohistochemistry Analysis: 1:250 dilution from a previous lot detected pro-caspase-3 in human colon adenocarcinoma tissue.

生物信息
免疫原品种	Synthetic peptide corresponding to residues following Ser29 of human caspase-3 (N-terminus of p17 subunit).
表位	N-terminus
克隆	E83-103
宿主	Rabbit
特异性	This antibody only detects pro-form (35kD) of caspase-3, and does not recognize any cleaved caspases.
同种型	IgG
品种反应性	MouseHuman
Species Reactivity Note	Human
抗体类型	Monoclonal Antibody
Entrez基因编号	NP_004337.2
Entrez基因汇总	This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. [provided by RefSeq].
基因符号	procaspase3 Apopain CASP-3 CPP-32 CPP32 CPP32B EC 3.4.22.56 OTTHUMP00000165054 SCA-1 Yama apopain
纯化方法	Unpurified
UniProt编号	P42574
UniProt汇总	FUNCTION: Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp-\|-Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop-helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin. CATALYTIC ACTIVITY: Strict requirement for an Asp residue at positions P1 and P4. It has a preferred cleavage sequence of Asp-Xaa-Xaa-Asp-\|- with a hydrophobic amino-acid residue at P2 and a hydrophilic amino-acid residue at P3, although Val or Ala are also accepted at this position. ENZYME REGULATION: Inhibited by isatin sulfonamides. SUBUNIT STRUCTURE: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit. Subcellular location Cytoplasm. TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system. PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa. S-nitrosylated on its catalytic site cysteine in unstimulated human cell lines and denitrosylated upon activation of the Fas apoptotic pathway, associated with an increase in intracellular caspase activity. Fas therefore activates caspase-3 not only by inducing the cleavage of the caspase zymogen to its active subunits, but also by stimulating the denitrosylation of its active site thiol. SEQUENCE SIMILARITY: Belongs to the peptidase C14A family.

产品使用声明
质量保证	Evaluated by Western Blotting on Jurkat cell lysates. Western Blotting Analysis: 1:500 dilution of this antibody was used to detect pro-caspase-3 in Jurkat cell lysate.
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Stable for 1 year at -20ºC from date of receipt. Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.

储存和货运信息

存储条件

Stable for 1 year at -20ºC from date of receipt.
Handling Recommendations: Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance. Note: Variability in freezer temperatures below -20°C may cause glycerol containing solutions to become frozen during storage.