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merck millipore,默克密理博,MABC32,Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2
产品名称：Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2
产品型号：MABC32
Detect p62 (Sequestosome-1) using this Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 validated for use in WB, FC & IC.
merck millipore,默克密理博,MABC32,Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 M, H, R ICC, WB, FC M Purified Monoclonal Antibody 描述 产品目录编号 MABC32 描述 Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 Alternate Names - sequestosome-1
- sequestosome 1
- EBI3-associated protein of 60 kDa
- Paget disease of bone 3
- phosphotyrosine independent ligand for the Lck SH2 domain p62
- oxidative stress induced like
- EBI3-associated protein p60
- Phosphotyrosine-independent ligand for the Lck SH2 domain of 62 kDa
- Ubiquitin-binding protein p62
背景信息 p62 is also known as sequestosome-1, and is a 62 kDa ras-GAP associated protein. p62 is thought to bind to src-family tyrosine kinases and may also provide a linker action for activated src-family kinases with their downstream effectors. p62 has an RNA-binding region and may have RNA-binding ability. p62 is associated with activation of transcription factor NF-κB, involved in cell differentiation, immune response and potassium channel regulation. p62 has recently been linked to extrinsic apoptosis and may be a key player in tumorigenesis. 产品信息 格式 Purified 控制 - A431 cell lysate
演示 Purified mouse monoclonal IgMκ in buffer containing PBS with 0.05% sodium azide. 应用 应用 Detect p62 (Sequestosome-1) using this Anti-p62 (Sequestosome-1) Antibody, clone 11C9.2 validated for use in WB, FC & IC. 主要应用 - Immunocytochemistry
- Western Blotting
- Flow Cytometry
应用说明 Western Blot Analysis: 0.001 µg/mL from a previous lot detected p62 (Sequestosome-1) in 10 µg of PC12 cell lysate.
Flow Cytometry Analysis: 1.0 µg from a previous lot detected p62 (Sequestosome-1) in the staining of fixed and permeabilized HeLa cells.
Immunocytochemistry Analysis: A 1:500 dilution from a previous lot detected p62 (Sequestosome-1) in NIH/3T3, A431, and HeLa cells.生物信息 免疫原品种 GST-tagged recombinant protein corresponding to human p62 (Sequestosome-1). 克隆 11C9.2 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 同种型 IgMκ 品种反应性 MouseHumanRat Species Reactivity Note Demonstrated to react with human, mouse, and rat. 抗体类型 Monoclonal Antibody Entrez基因编号 - NP_001135770
Entrez基因汇总 This gene encodes a multifunctional protein that binds ubiquitin and regulates activation of the nuclear factor kappa-B (NF-kB) signaling pathway. The protein functions as a scaffolding/adaptor protein in concert with TNF receptor-associated factor 6 to mediate activation of NF-kB in response to upstream signals. Alternatively spliced transcript variants encoding either the same or different isoforms have been identified for this gene. Mutations in this gene result in sporadic and familial Paget disease of bone. [provided by RefSeq]. 基因符号 - P62
- PDB3
- p62B
- p62
- A170
- ZIP3
- SQSTM1
- p60
- OSIL
- ORCA
- EBIAP
纯化方法 Ion Exchange UniProt编号 - Q13501
UniProt汇总 FUNCTION: Adapter protein which binds ubiquitin and may regulate the activation of NFKB1 by TNF-alpha, nerve growth factor (NGF) and interleukin-1. May play a role in titin/TTN downstream signaling in muscle cells. May regulate signaling cascades through ubiquitination. Adapter that mediates the interaction between TRAF6 and CYLD (By similarity). May be involved in cell differentiation, apoptosis, immune response and regulation of K+ channels.
SUBUNIT STRUCTURE: Homooligomer or heterooligomer; may form homotypic arrays. Interacts directly with PRKCI and PRKCZ Probable. Forms ternary complexes with PRKCZ and KCNAB2 or PRKCZ and GABBR3. Also interacts with KCNAB1, GABRR1, GABRR2 and GABRR3. Forms an NGF-induced complex with IKBKB, PRKCI and TRAF6 (By similarity). Interacts with EBI3, LCK, RASA1, PRKCZ, PRKCI, NR2F2, NTRK1, NTRK2, NTRK3, NBR1, MAP2K5, TRIM55 and MAPKAPK5. Interacts with the proteasome subunits PSMD4 and PSMC2. Interacts with K63-polyubiquitinated MAPT/TAU. Interacts with IKBKB through PRKCZ and PRKCI. Interacts with NGFR through TRAF6 and bridges that complex to NTRK1. Forms a complex with MAP2K5 and PRKCZ or PRKCI. Component of a ternary complex with PAWR and PRKCZ. Upon TNF-alpha stimulation, interacts with RIPK1 problably bridging IKBKB to the TNF-R1 complex composed of TNF-R1/TNFRSF1A, TRADD and RIPK1. Forms a complex with JUB/Ajuba, PRKCZ and TRAF6. Interacts with TRAF6 and CYLD. Identified in a complex with TRAF6 and CYLD (By similarity).
SUBCELLULAR LOCATION: Cytoplasm. Late endosome. Nucleus. Note: Sarcomere (By similarity). In cardiac muscles localizes to the sarcomeric band (By similarity). Localizes to late endosomes. May also localize to the nucleus. Accumulates in neurofibrillary tangles and in Lewy bodies of neurons from individuals with Alzheimer and Parkinson disease respectively. Enriched in Rosenthal fibers of pilocytic astrocytoma. In liver cells, accumulates in Mallory bodies associated with alcoholic hepatitis, Wilson disease, indian childhood cirrhosis and in hyaline bodies associated with hepatocellular carcinoma.
TISSUE SPECIFICTY: Ubiquitously expressed.
INDUCTION: By proteasomal inhibitor PSI and prostaglandin J2 (PGJ2) (at protein level). By phorbol 12-myristate 13-acetate (PMA).
DOMAIN: The UBA domain binds specifically 'Lys-63'-linked polyubiquitin chains of polyubiquitinated substrates. Mediates the interaction with TRIM55.
The OPR domain mediates homooligomerization and interactions with PRKCZ, PRKCI, MAP2K5 and NBR1.
The ZZ-type zinc finger mediates the interaction with RIPK1.
PTM: Phosphorylated. May be phosphorylated by PRKCZ (By similarity). Phosphorylated in vitro by TTN.
INVOLVEMENT IN DISEASE: Defects in SQSTM1 are a cause of Paget disease of bone (PDB) [MIM:602080]. PDB is a metabolic bone disease affecting the axial skeleton and characterized by focal areas of increased and disorganized bone turn-over due to activated osteoclasts. Manifestations of the disease include bone pain, deformity, pathological fractures, deafness, neurological complications and increased risk of osteosarcoma. PDB is a chronic disease affecting 2 to 3% of the population above the age of 40 years.
SEQUENCE SIMILARITIES: Contains 1 OPR domain.
Contains 1 UBA domain.
Contains 1 ZZ-type zinc finger.产品使用声明 质量保证 Evaluated by Western Blot in A431 cell lysate.
Western Blot Analysis: 0.001 µg/mL of this antibody detected p62 (Sequestosome-1) in 10 µg of A431 cell lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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