merck millipore,默克密理博,03-227,HDAC3/NCOR1 complex human recombinant

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merck millipore,默克密理博,03-227,HDAC3/NCOR1 complex human recombinant
产品名称：HDAC3/NCOR1 complex human recombinant
产品型号：03-227
Human recombinant HDAC3/NCOR1 produced in insect cells.

merck millipore,默克密理博,03-227,HDAC3/NCOR1 complex human recombinant

产品介绍

merck millipore,默克密理博,03-227,HDAC3/NCOR1 complex human recombinant

重要规格表

主要应用	品种
EA	Human

描述
产品目录编号	03-227
品牌系列	Upstate
商名	Upstate
描述	HDAC3/NCOR1 complex human recombinant
概述	Human recombinant HDAC3/NCOR1 produced in insect cells
背景信息	Unlike other class I HDAC enzymes, HDAC3 is found in co-repressor complexes with the SMRT/N-CoR proteins. HDAC3 contains a unique domain structure which includes both nuclear localization and nuclear export sequences. HDAC3 has been implicated in cancer, as HDAC3 inhibitors produce anti-proliferative effects in various cancer cells. It may also play a role in heart disease, which may involve deacetylation of MEF2.

产品信息
格式	Purified
演示	In liquid containing 50 mM Tris, pH 8.0, 138 mM NaCl and 10% glycerol.

应用
应用	Human recombinant HDAC3/NCOR1 produced in insect cells.
主要应用	Enzyme Assay
应用说明	HDAC deacetylase assays

生物信息
纯度	Purity >90% by SDS-PAGE
来源	Human HDAC3 and the activation domain of human NCOR1
品种	Human
特异活性	>150 U/µg (based on total protein;>250 U/µg based on weight of HDAC3). 1U = 1 pmol/min at 37°C using a standard flourometric activity assay to measure deacetylation activity
物理形式	Frozen solution

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC3/NCOR1 into separate tubes before storing at -80°C.

储存和货运信息

存储条件

Store at -80°C. The enzyme is stable on ice for 30 to 60 min, but may lose activity with dilution and/or prolonged storage on ice. It is recommended that thawing and addition of the enzyme to assay wells be done within as short a time as possible before start of the assay and that the thawed enzyme be kept on ice throughout. Best performance will be obtained by adding the chilled, undiluted enzyme directly to pre-warmed buffer and proceeding immediately to the addition of pre-warmed substrate. The remaining, unused enzyme should be refrozen quickly by, for example, snap freezing in a dry/ice ethanol bath or liquid nitrogen. The enzyme is stable to at least 4 freeze/thaw cycles. To minimize the number of freeze/thaw cycles, aliquot the HDAC3/NCOR1 into separate tubes before storing at -80°C.