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品种反应性 主要应用 宿主 格式 抗体类型 Ma IHC, FC, ICC M Purified Monoclonal Antibody 描述 产品目录编号 MAB312 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-A2B5 Antibody, clone A2B5-105 Alternate Names - Neuron Cell Surface Antigen
产品信息 格式 Purified 控制 - Type II astrocytes, human neural progenitors
演示 Liquid in 0.02M PB, 0.25M NaCl, pH 7.6 containing 0.1% sodium azide. 应用 应用 Detect A2B5 using this Anti-A2B5 Antibody, clone A2B5-105 validated for use in FC, IC, IF, IH. 主要应用 - Immunohistochemistry
- Flow Cytometry
- Immunocytochemistry
应用说明 Indirect immunofluorescence (1:100 - 1:500) in the detection of neurons in tissue culture.
Using double immunofluorescence the GQ ganglioside has a similar distribution on neurons to the D2 protein, to the tetanus toxin receptor and to neurofilaments (Walsh, 1980).
May also be used in the depletion of neurons for a mixed population or their purification by affinity chromatography.
May also be useful for detecting the metastatic spread of neuroblastoma cells into bone marrow.
Flow cytometry: live cells {Maric, D. et al. (2000) Cerebral Cortex 10:729-747}.
Immunohistochemistry: Frozen, fixed tissues (Levison & McCarthy, 1989)
Complement-mediated cytotoxicity (Eisenbarth et al., 1979)
Optimal working dilutions must be determined by end user.生物信息 免疫原品种 Embryonic chicken retinal cells 克隆 A2B5-105 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 Reacts with a surface antigen on neurons in the retina, brain, spinal cord and dorsal root ganglia, and with all tested human neuroblastoma cell lines. Does not seem to bind with any leukemic cell lines or with bone marrow cells from patients with leukemia, nor with cells from normal bone marrow. Cytotoxic to neurons in the presence of guinea-pig complement. Protein A binding is positive. Binds with GQ ganglioside on the plasma membrane of neurons of animals of all species tested to date (Eisenbarth, et al., 1979). 同种型 IgM 品种反应性 Mammals 抗体类型 Monoclonal Antibody 基因符号 - A2B5
纯化方法 Ammonium sulfate precipitation 产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain for 1 year at 2–8°C from date of shipment. Aliquot to avoid repeated freezing and thawing. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. 包装信息 数量 100 µg
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