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宿主 M 描述 概述 Recognizes the ~20-22 kDa DHFR protein in HeLa and PC-12 cells. 产品目录编号 ST1711 品牌系列 Calbiochem®
同义词 Anti-Dihydrofolate Reductase 应用数据 
Detection of human DHFR by immunoblotting. Sample: Whole cell lysate from HeLa cells. Primary antibody: Anti-DFHR Mouse mAb (2B10) (Cat. No. ST1711) (1-5 µg/ml). Detection: chemiluminescence.
Detection of rat DHFR by immunoblotting. Sample: Whole cell lysate from PC-12 cells. Primary antibody: Anti-DFHR Mouse mAb (2B10) (Cat. No. ST1711) (1-5 µg/ml). Detection: chemiluminescence.
Detection of human DHFR by immunocytochemistry. Sample: HeLa cells fixed in paraformaldehyde. Primary antibody: Anti-DFHR Mouse mAb (2B10) (Cat. No. ST1711) (10 µg/ml). Detection: fluorescence.参考 参考 Vodenicharov, M.D., et al. 2010. The EMBO Journal 29, 3007.
Ionova, I.A., et al. 2008. Am. J. Physiol. Heart Circ. Physiol. 295 H2178.产品信息 形式 liquid 配方 In PBS, pH 7.2. 负控制 293T cells 正控制 HeLa cells, PC-12 Cells 防腐剂 None 应用 主要应用 Enzyme-Linked Immunosorbent Assay
Immunoblotting (Western Blotting)
Immunocytochemistry
生物信息 免疫原 A recombinant polypeptide corresponding to amino acids of 88-188 human DHFR, expressed as a GST fusion protein 克隆 2B10 宿主 Mouse 同种型 IgG2a 品种 human, rat 储存和货运信息 货运代码 Blue Ice Only
毒性 Regulatory Review
储存 -20°C
避免冻结 / 解冻 Avoid freeze/thaw
无需冷冻 No
特殊指南 Following initial thaw, aliquot and freeze (-20°C).
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