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品种反应性 主要应用 宿主 格式 抗体类型 M, H IHC M Ascites Monoclonal Antibody 描述 产品目录编号 MABN671 描述 Anti-DLK1 Antibody, clone 3A10 Alternate Names - Pref-1
- Protein delta homolog 1
- DLK-1
- FA1
- ZOG
- pG2
- PREF1
背景信息 Protein delta homolog 1or DLK-1 (DLK1), also known as pG2 and the Preadipocyte factor-1 (Pref-1), is a single pass type 1 membrane protein known to play a steering role in neuroendocrine differentiation. The protein is expressed within the stromal cells in contact with the vascular structure of the placental villi, yok sac, fetal liver, adrenal cortex and pancreas during development and DLK1 is also expressed within the beta cells of the islets of Langerhans in the adult. DLK1 plays an important gene regulatory expression role during the development of not only these very early structures but also in the regulation of the development of many specialized cells and tissues such as adipocytes. In adipose tissue, DLK1/Pref-1 is specifically expressed in preadipocytes but not in adipocytes and thus can be used as a preadipocyte marker. The inhibition operates through the creation of a soluble cleaved cytoplasmic form of DLK1 via the action of the metalloproteinase ADAM17 on mature DLK1. Fetal Antigen 1 (FA1) or soluble DLK-1/Pref-1 activates the MAPK kinase/ERK pathway, and via Sox9 interactions ultimately inhibits the differentiation of pre-adipocytes into mature adipocytes. Furthermore, by inducing Sox9, Pref-1 promotes chondrogenic induction of mesenchymal cells but prevents chondrocyte maturation as well as osteoblast differentiation. Thus, DLK1/Pref-1 directs multipotent mesenchymal cells (MSCs) toward the chondrogenic lineage but inhibits differentiation into adipocytes as well as osteoblasts and chondrocytes. Naturally, DLK1 has multiple isoforms each with slightly differing but related influences on MSCs development. Finally, DLK1 is also expressed in various tumors including small cell lung carcinoma and numerous neuroendocrine cancers. 产品信息 格式 Ascites 控制 - Human pancreas tissue
演示 Mouse monoclonal IgG1 ascitic fluid containing up to 0.1% sodium azide. 应用 应用 Detect DLK1 using this mouse monoclonal antibody, Anti-DLK1 Antibody, clone 3A10 validated for use in IHC & Immunofluorescence. 主要应用 - Immunohistochemistry
应用说明 Immunofluorescence Analysis: A 1:200-1,000 dilution from a representative lot detected DLK1 in U251 cells.
Optimal working dilutions must be determined by end user.
生物信息 免疫原品种 Purified recombinant fragment of human DLK1 expressed in E. Coli. 克隆 3A10 宿主 Mouse 同种型 IgG1 品种反应性 MouseHuman 抗体类型 Monoclonal Antibody 基因符号 - DLK1
- DLK
纯化方法 Unpurified UniProt编号 - P80370
产品使用声明 质量保证 Evaluated by Immunohistochemistry Analysis in human pancreas tissue.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected DLK1 in human pancreas tissue.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at -20°C from date of receipt.
Handling Recommendations: Upon receipt and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C. Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.包装信息 数量 100 µL
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