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merck millipore,默克密理博,SF016-200,ESGRO-2i Medium, 200ml
产品名称：ESGRO-2i Medium, 200ml
产品型号：SF016-200
The EsGRO-2i medium is suitable for ES & iPS cell derivation, passaging & maintenance. This media can be used on feeder dependent & independent pluirpotent stem cell lines.
merck millipore,默克密理博,SF016-200,ESGRO-2i Medium, 200ml
- 产品介绍
重要规格表
主要应用 Cult 描述 产品目录编号 SF016-200 描述 ESGRO-2i Medium, 200ml 概述 ESGRO-2i medium is a defined, LIF containing medium provided with a selective GSK3β and Mek 1/2 inhibitors to enhance viability of mouse embryonic stem (ES) and induced pluripotent stem (iPS) cells and increased maintenance of pluripotency in the absence of serum and feeder cells. This medium is intended for cell expansion and growth at clonal density in serum-free conditions. 产品信息 部件 - 3. Mek1/2 Inhibitor Supplement: (Catalogue No. CS204465) Two (2) 10 μL vials containing selective Mek 1/2 inhibitor supplement.
- 1. ESGRO-2i Basal Medium with LIF: (Catalogue No. CS204463) Two (2) 100 mL bottles containing basal medium supplemented with LIF, pH 7.1-7.3.
- 2. GSK3β Inhibitor Supplement: (Catalogue No. SF012-050) Two (2) 50 μL vials containing selective GSK3β inhibitor supplement.
应用 应用 The EsGRO-2i medium is suitable for ES & iPS cell derivation, passaging & maintenance. This media can be used on feeder dependent & independent pluirpotent stem cell lines. 主要应用 - Stem Cell Culture
应用说明 Suitable for ES and iPS cell derivation, passaging and maintenance. Media can be used on feeder dependent and independent pluirpotent stem cell lines.
Important passaging information
Use of one of the below listed cell dissociation solutions is imperative while passaging ES cells. Standard trypsin protocols used to passage ES cells grown in serum containing media typically result in over trypsinization causing the cells to lift off the plates and form abnormal ball-like structures.
Recommended products include:
ESGRO Complete™ Accutase (Cat # SF006)
ESGRO Complete™ Trypsin Solution (proprietary) (Cat # SF007)
ESGRO Complete™ Enzyme-Free Dissociation Solution (proprietary) (Cat # SF009)产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 • Upon receipt, store basal medium and inhibitor supplements in the dark at -20°C until use. Refer to lot expiration date on labels.
• After basal medium and inhibitor supplements are combined, store the complete ESGRO-2i medium in the dark at 4°C for up to 10 days. Do not refreeze.
包装信息 数量 200 mL
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