merck millipore,默克密理博,CYT304,ChemiKine Nerve Growth Factor, Sandwich ELISA

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merck millipore,默克密理博,CYT304,ChemiKine Nerve Growth Factor, Sandwich ELISA
产品名称：ChemiKine Nerve Growth Factor, Sandwich ELISA
产品型号：CYT304
This ChemiKine Nerve Growth Factor, Sandwich ELISA is used to measure & quantify NGF levels in Neuroscience research.

merck millipore,默克密理博,CYT304,ChemiKine Nerve Growth Factor, Sandwich ELISA

产品介绍

merck millipore,默克密理博,CYT304,ChemiKine Nerve Growth Factor, Sandwich ELISA

重要规格表

可用的分析物	品种反应性	主要应用	检测方法
NGF	R, M	ELISA	Chromogenic

描述
产品目录编号	CYT304
品牌系列	Chemicon®
商名	Chemicon ChemiKine
描述	ChemiKine Nerve Growth Factor, Sandwich ELISA
概述	With the ChemiKine NGF assay system, sheep polyclonal antibodies generated against mouse NGF are coated onto a microplate and are used to capture NGF from a sample. NGF specific mouse monoclonal antibodies detect the captured NGF. Mouse specific donkey polyclonal antibodies, peroxidase labeled, detect the mouse monoclonal antibody. After addition of the substrate and stop solution the amount of NGF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and NGF concentration: i.e., the higher the OD the higher the NGF concentration in the sample.ChemiKine NGF kit is designed to measure the amount of NGF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from mouse and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. The kit utilizes strip well inserts. Running duplicate wells for samples and standards is recommended. Analytical Sensitivity and Detection Limits: Sensitivity:10-15 pg/mL Range of Detection: 10 pg/mL to 500 pg/mL Crossreactivity: No significant cross reactivity with BDNF, NT4/5 or NT3 Intra-assay Variation:± 4.1% (250 pg/mL) Inter-assay Variation:± 8.9 % (250 pg/mL) Test Principle: With the ChemiKine NGF assay system, sheep polyclonal antibodies generated against mouse NGF are coated onto a microplate and are used to capture NGF from a sample. NGF specific mouse monoclonal antibodies detect the captured NGF. Mouse specific donkey polyclonal antibodies, peroxidase labeled, detect the mouse monoclonal antibody. After addition of the substrate and stop solution the amount of NGF is determined. The standard curve demonstrates a direct relationship between Optical Density (OD) and NGF concentration: i.e., the higher the OD the higher the NGF concentration in the sample. Application: ChemiKine NGF kit is designed to measure the amount of NGF in cell culture supernatants, tissue homogenates and biological fluid (serum, plasma, and serum-free) samples from mouse and rat. There are enough reagents included in this kit for two 96-well immuno-assay plates. Running duplicate wells for samples and standards is recommended. For Research Use Only. Not for use in diagnostic procedures.
Alternate Names	NGF

产品信息
部件	Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution (Ready to Use). ChemiKine NGF ELISA Plate: (Part No. 90139) Two Pre-coated with Sheep anti-Mouse NGF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches. Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate. Assay Diluent: (Part No. 90143) One 100 mL bottle (Ready to Use). NGF Standard (Mouse): (Part No. 90142) Two vials (Lyophilized). Mouse anti-Mouse NGF Monoclonal Antibody: (Part No. 90141) Two vials (Lyophilized). Donkey anti-Mouse IgG Polyclonal Antibody, HRP conjugated: (Part No. 90140) Two vials (Lyophilized). TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.
检测方法	Chromogenic

产品信息

部件

Stop Solution (Part No. 60260): One 22 mL bottle of an HCl solution (Ready to Use).
ChemiKine NGF ELISA Plate: (Part No. 90139) Two Pre-coated with Sheep anti-Mouse NGF Polyclonal Antibody 96-Well Immunoplates sealed in foil pouches.
Wash Buffer Concentrate: (Part No. 60245) One 100 mL (10X) bottle of Concentrate.
Assay Diluent: (Part No. 90143) One 100 mL bottle (Ready to Use).
NGF Standard (Mouse): (Part No. 90142) Two vials (Lyophilized).
Mouse anti-Mouse NGF Monoclonal Antibody: (Part No. 90141) Two vials (Lyophilized).
Donkey anti-Mouse IgG Polyclonal Antibody, HRP conjugated: (Part No. 90140) Two vials (Lyophilized).
TMB/E Solution: (Part No. 60096) Two 10 mL bottles of a Ready to Use solution of 3,3',5,5'-tetramethylbenzidine in a proprietary buffer with enhancer.

检测方法

Chromogenic

应用
应用	This ChemiKine Nerve Growth Factor, Sandwich ELISA is used to measure & quantify NGF levels in Neuroscience research.
主要应用	ELISA

生物信息
品种反应性	RatMouse
可用的分析物	NGF
Entrez基因编号	NM_002527.4 NM_001102654.1
Entrez基因汇总	The protein encoded by this gene is a member of the neurotrophin family, that controls survival and differentiation of mammalian neurons. This protein is closely related to both nerve growth factor and brain-derived neurotrophic factor. It may be involved in the maintenance of the adult nervous system, and may affect development of neurons in the embryo when it is expressed in human placenta. NTF3-deficient mice generated by gene targeting display severe movement defects of the limbs. The mature peptide of this protein is identical in all mammals examined including human, pig, rat and mouse.
基因符号	NGFB NGF
UniProt编号	P01139

产品使用声明
使用声明	Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

储存和货运信息
存储条件	Precautions: · Wash Buffer and Assay Diluent contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly. · The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.

储存和货运信息

存储条件

Precautions:

· Wash Buffer and Assay Diluent contain thimerosal. Thimerosal is highly toxic by inhalation, contact with skin or if swallowed. Thimerosal is a possible mutagen and should be handled accordingly.

· The instructions provided have been designed to optimize the kit's performance. Deviation from the instructions may result in suboptimal performance of the kit and the failure to produce accurate data.