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品种反应性 主要应用 宿主 格式 抗体类型 M, H WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB3640 Replaces 04-1090; 04-439 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Caspase 3 Antibody Alternate Names - SCA-1
- CPP32
- Apopain
- Yama protein
背景信息 Caspase-3 is the most extensively studied apoptotic protein. Caspase-3 is synthesized as an inactive proenzyme (~32-35 kDa) that is processed in cells undergoing apoptosis by self-proteolysis and/or cleavage by another upstream protease. The processed form of caspase-3 consists of large (17 kDa) and small (12 kDa) subunits which associate to form an active enzyme. The active caspase-3 proteolytically cleaves and activates other caspases, as well as relevant targets in the cells such as PARP and DFF. 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in PBS, pH 7.6 and 0.1% sodium azide as a preservative. 应用 应用 Detect Caspase 3 using this Anti-Caspase 3 Antibody validated for use in WB. 主要应用 - Western Blotting
应用说明 Western Blot: 1:500 - 1:1000 生物信息 免疫原品种 Peptide corresponding to a.a. 104-117 of human Caspase 3 表位 a.a. 104-117 宿主 Rabbit 特异性 AB3640 recognizes a region within the p17 large subunit of Caspase 3. The antibody will detect both pro- and large subunit forms of the protein, as well as partially processed intermediates in apoptotically induced cells. 品种反应性 MouseHuman 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_032991.2
- NM_004346.3
Entrez基因汇总 This gene encodes a protein which is a member of the cysteine-aspartic acid protease (caspase) family. Sequential activation of caspases plays a central role in the execution-phase of cell apoptosis. Caspases exist as inactive proenzymes which undergo proteolytic processing at conserved aspartic residues to produce two subunits, large and small, that dimerize to form the active enzyme. This protein cleaves and activates caspases 6, 7 and 9, and the protein itself is processed by caspases 8, 9 and 10. It is the predominant caspase involved in the cleavage of amyloid-beta 4A precursor protein, which is associated with neuronal death in Alzheimer's disease. Alternative splicing of this gene results in two transcript variants that encode the same protein. 基因符号 - Caspase-3 subunit p12].
- CASP3
- apopain
- procaspase3
- CPP-32
- Apopain
- Yama
- CASP-3
- CPP32B
- SCA-1
- CPP32
- EC 3.4.22.56 [Contains: Caspase-3 subunit p17
纯化方法 Immunoaffinity purified UniProt编号 - P42574
UniProt汇总 FUNCTION: SwissProt: P42574 # Involved in the activation cascade of caspases responsible for apoptosis execution. At the onset of apoptosis it proteolytically cleaves poly(ADP-ribose) polymerase (PARP) at a '216-Asp- -Gly-217' bond. Cleaves and activates sterol regulatory element binding proteins (SREBPs) between the basic helix-loop- helix leucine zipper domain and the membrane attachment domain. Cleaves and activates caspase-6, -7 and -9. Involved in the cleavage of huntingtin.
SIZE: 277 amino acids; 31608 Da
SUBUNIT: Heterotetramer that consists of two anti-parallel arranged heterodimers, each one formed by a 17 kDa (p17) and a 12 kDa (p12) subunit.
SUBCELLULAR LOCATION: Cytoplasm.
TISSUE SPECIFICITY: Highly expressed in lung, spleen, heart, liver and kidney. Moderate levels in brain and skeletal muscle, and low in testis. Also found in many cell lines, highest expression in cells of the immune system.
PTM: Cleavage by granzyme B, caspase-6, caspase-8 and caspase-10 generates the two active subunits. Additional processing of the propeptides is likely due to the autocatalytic activity of the activated protease. Active heterodimers between the small subunit of caspase-7 protease and the large subunit of caspase-3 also occur and vice versa.
SIMILARITY: SwissProt: P42574 ## Belongs to the peptidase C14 family.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquotes for up to 12 months from date of receipt. 包装信息 数量 100 µg
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