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merck millipore,默克密理博,HTS083M,ChemiScreen™ Recombinant Human CCK2 Cholecystokinin Receptor Membrane Preparation
产品名称：ChemiScreen™ Recombinant Human CCK2 Cholecystokinin Receptor Membrane Preparation
产品型号：HTS083M
Human CCK2 / CCKb GPCR membrane preparation for Radioligand binding Assays & GTPγS binding.
merck millipore,默克密理博,HTS083M,ChemiScreen™ Recombinant Human CCK2 Cholecystokinin Receptor Membrane Preparation
- 产品介绍
- merck millipore,默克密理博,HTS083M,ChemiScreen™ Recombinant Human CCK2 Cholecystokinin Receptor Membrane Preparation
重要规格表
应用说明 品种 宿主细胞 蛋白靶 Radioligand binding assay and GTPγS binding. Human Chem-1 CCK2 / CCKb 描述 产品目录编号 HTS083M 品牌系列 Chemicon®
商名 - Chemicon
- ChemiScreen
描述 ChemiScreen™ Recombinant Human CCK2 Cholecystokinin Receptor Membrane Preparation 概述 Full-length human CCKB cDNA encoding CCK2 背景信息 Cholecystokinins are a series of peptides of heterogeneous length (5 to 58 amino acids) that are derived from preprocholecystokinin and are found in gastrointestinal tissues and the central nervous system. Gastrin is a related peptide with 5 C-terminal amino acids identical to those of cholecystokinin. Two GPCRs, CCK1 (CCKA) and CCK2 (CCKB), bind to CCK and/or gastrin to mediate the biological effects of the peptides. CCK2 binds to CCK and gastrin with similar affinity, whereas CCK1 selectively binds sulfated CCK. Binding of ligands to CCK2 stimulates mobilization of intracellular calcium by activation of Gq/11. In the periphery, CCK2 is present in the stomach, where it mediates gastrin-stimulated gastric acid secretion. In the CNS, CCK2 has been implicated in anxiety, depression, schizophrenia, depression and opioid analgesia (Noble et al., 1999). Chemicon's CCK2 membrane preparations are crude membrane preparations made from our proprietary stable recombinant cell lines to ensure high-level of GPCR surface expression; thus, they are ideal HTS tools for screening of antagonists of CCK2 interactions and its ligands. The membrane preparations exhibit a Kd of 0.47 nM for [125I]-cholecystokinin octapeptide (CCK-8). With 0.3 nM [125I]CCK-8, 5 or 10 μg/well CCK2 Membrane Prep yields greater than 20-fold signal-to-background ratio. 产品信息 演示 Liquid in packaging buffer: 50 mM Tris pH 7.4, 10% glycerol and 1% BSA with no preservatives.
Packaging method: Membranes protein were adjusted to 0.5 mg/ml in 1 ml packaging buffer, rapidly frozen, and stored at -80°C.应用 应用 Human CCK2 / CCKb GPCR membrane preparation for Radioligand binding Assays & GTPγS binding. 应用说明 Radioligand binding assay and GTPγS binding. 生物信息 品种 Human 品种反应性 Human Entrez基因编号 - NM_176875.2
Entrez基因汇总 This gene encodes a G-protein coupled receptor for gastrin and cholecystokinin (CCK), regulatory peptides of the brain and gastrointestinal tract. This protein is a type B gastrin receptor, which has a high affinity for both sulfated and nonsulfated CCK analogs and is found principally in the central nervous system and the gastrointestinal tract. A misspliced transcript variant including an intron has been observed in cells from colorectal and pancreatic tumors. 基因符号 - CCK2-R
- CCKBR
- CCK-BR
- CCK2R
- CCK-B
- GASR
- CCKRB
GPCR类别 A 宿主细胞 Chem-1 蛋白靶 CCK2 / CCKb 靶子系列 Cholecystokinin UniProt编号 - P32239
UniProt汇总 FUNCTION: SwissProt: P32239 # Receptor for gastrin and cholecystokinin. The CKK-B receptors occur throughout the central nervous system where they modulate anxiety, analgesia, arousal, and neuroleptic activity. This receptor mediates its action by association with G proteins that activate a phosphatidylinositol-calcium second messenger system.
SIZE: 447 amino acids; 48419 Da
SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
SIMILARITY: SwissProt: P32239 ## Belongs to the G-protein coupled receptor 1 family.孵化条件 Membranes are mixed with radioactive ligand and unlabeled competitor (see Figures 1 and 2 for concentrations tested) in binding buffer in a nonbinding 96-well plate, and incubated for 1-2 h. Prior to filtration, a GF/C 96-well filter plate is coated with 0.33% polyethyleneimine for 30 min, then washed with 50mM HEPES, pH 7.4, 0.5% BSA. Binding reaction is transferred to the filter plate, and washed 3 times (1 mL per well per wash) with Wash Buffer. The plate is dried and counted.
• Binding buffer: 50 mM Tris, pH 7.4, 10 mM MgCl2, 1 mM EDTA, filtered and stored at 4°C
• Radioligand: [125I]-CCK-8 (Perkin Elmer#:NEX-203 )
• Wash Buffer: 50 mM Tris, pH 7.4 filtered and stored at 4°C.
One package contains enough membranes for at least 200 assays (units), where a unit is the amount of membrane that will yield greater than 20-fold signal:background with 125I labeled CCK-8 at 0.3 nM产品使用声明 质量保证 Signal:background and specific binding values obtained in a competition binding assay with varying amounts of CCK2 membrane prep:
10 µg/well 5 µg/well Signal:Background 27.4 27.8 Specific Binding (cpm) 25,302 20,111
Specifications:
1unit = 5 μg
Bmax for [125I] CCK-8 binding: 3.47 pmol/mg protein
Kd for [125I] CCK-8 binding: ~0.47 nM使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain frozen at -70°C for up to 2 years. Do not freeze and thaw. 包装信息 数量 200 units
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