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品种反应性 主要应用 宿主 格式 抗体类型 R, H, M IHC, WB Rb Affinity Purified Polyclonal Antibody 描述 产品目录编号 AB9372 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Necdin Antibody 产品信息 格式 Affinity Purified 演示 Affinity purified immunoglobulin. Liquid in 0.02M Phosphate buffer, 0.25M NaCl with 0.1% sodium azide. 应用 应用 This Anti-Necdin Antibody is validated for use in WB, IH for the detection of Necdin. 主要应用 - Immunohistochemistry
- Western Blotting
应用说明 Immunoblotting: 0.5-1.0 μg/mL on mouse brain tissue homogenate. The antibody reacts with the ~43 kD Necdin protein. Additional bands at ~35 kD and ~60 kD may been seen depending on sample and antibody concentration used.
Immunohistochemistry: 1:100 mouse cerebral cortex (second somatosensory cortex) using HIER (heat induced epitope retrieval). Recommend using citrate, pH 6.0.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 KLH-conjugated linear peptide of human Necdin at the N-terminus. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 特异性 Recognizes Necdin. 品种反应性 RatHumanMouse Species Reactivity Note Proven to react with human, mouse, and rat. Predicted to react with rhesus monkey based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NM_002487.2
Entrez基因汇总 This intronless gene is located in the Prader-Willi syndrome deletion region. It is an imprinted gene and is expressed exclusively from the paternal allele. Studies in mouse suggest that the protein encoded by this gene may suppress growth in postmitotic neurons. 基因符号 - Necdin.
- NDN
- HsT16328
- necdin
纯化方法 Affinity Purfied UniProt编号 - Q99608
UniProt汇总 FUNCTION: SwissProt: Q99608 # Growth suppressor that facilitates the entry of the cell into cell cycle arrest. Functionally similar to the retinoblastoma protein it binds to and represses the activity of cell-cycle- promoting proteins such as SV40 large T antigen, adenovirus E1A, and the transcription factor E2F. Necdin also interacts with p53 and works in an additive manner to inhibit cell growth. Functions also as transcription factor and binds directly to specific guanosine-rich DNA sequences (By similarity).
SIZE: 321 amino acids; 36086 Da
SUBUNIT: Binds to the transactivation domains of E2F1 and p53. Binds also SV40 large T antigen and adenovirus E1A. Interacts with nucleobindin 1 and 2 (By similarity).
SUBCELLULAR LOCATION: Perikaryon. Nucleus. Note=Neural perikarya, translocates to the nucleus of postmitotic neurons and interacts with the nuclear matrix.
TISSUE SPECIFICITY: Almost ubiquitous. Detected in fetal brain, lung, liver and kidney; in adult heart, brain, placenta, lung, liver, skeletal muscle, kidney, pancreas, spleen, thymus, prostate, testis, ovary, small intestine and colon. Not detected in peripheral blood leukocytes. In brain, restricted to post- mitotic neurons.
DISEASE: SwissProt: Q99608 # Defects in NDN may be a cause of the Prader-Willi syndrome (PWS) which is a contiguous gene syndrome resulting from inactivity of the paternal copies of a number of genes on 15q11, through deletion or disruption of these genes or maternal uniparental disomy 15. The PWS syndrome is characterized by muscular hypotonia, mental retardation, short stature, obesity, hypogonadotropic hypogonadism, and small hands and feet.
SIMILARITY: SwissProt: Q99608 ## Contains 1 MAGE domain.产品使用声明 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted aliquots for up to 6 months after date of receipt. 包装信息 数量 100 µg
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