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merck millipore,默克密理博,MAB347,Anti-Growth Associated Protein 43 Antibody, clone 9-1E12
产品名称：Anti-Growth Associated Protein 43 Antibody, clone 9-1E12
产品型号：MAB347
Anti-Growth Associated Protein 43 Antibody, clone 9-1E12 detects level of Growth Associated Protein 43 & has been published & validated for use in IH, IP & WB.
merck millipore,默克密理博,MAB347,Anti-Growth Associated Protein 43 Antibody, clone 9-1E12
- 产品介绍
重要规格表
品种反应性 主要应用 宿主 格式 抗体类型 Ht, H, R, Fe WB, IHC M Purified Monoclonal Antibody 描述 产品目录编号 MAB347 品牌系列 Chemicon®
商名 - Chemicon
描述 Anti-Growth Associated Protein 43 Antibody, clone 9-1E12 Alternate Names - neuromodulin
- Growth Associated Protein 43
- B-50 Protein
- Neuromodulin
背景信息 GAP-43, a common marker of differentiating neurons, is expressed at elevated levels by developing or regenerating neurons during axon growth. While GAP-43 is an integral membrane protein associated with the cytoplasmic surface of axonal growth cones (and synapses), it is absent from dendritic growth cones. In adult brain, GAP-43 is found in high concentration in presynaptic areas where memory formation is thought to occur, such as frontal cortex, limbic system and hippocampus. Phosphorylation of GAP-43 by PKC (at Ser41 on human, mouse, rat, and bovine GAP-43 or Ser42 on chicken and Xenopus GAP-43) is specifically correlated with certain forms of synaptic plasticity. 产品信息 格式 Purified 控制 - Rat DRG tissue that has been subjected to a spinal nerve ligation and cultured neurons.
演示 Protein A Purified mouse immunoglobulin IgG1 in 20 mM sodium phosphate, 250 mM NaCl, pH. 7.6, with 0.1% sodium azide as a preservative. 应用 应用 Anti-Growth Associated Protein 43 Antibody, clone 9-1E12 detects level of Growth Associated Protein 43 & has been published & validated for use in IH, IP & WB. 主要应用 - Western Blotting
- Immunohistochemistry
应用说明 Immunohistochemistry:
0.1-0.5 µg/mL of a previous lot on 4% paraformaldehyde perfused and fixed rat cerebellum and cerebrum tissue. The addition of 0.01-0.1% Triton X-100 to incubation buffer will increase cellular permeability.
Immunoprecipitation:
A previous lot of this antibody was used in immunoprecipitation.
5-10µg/mL in membrane preparations from 16-18 day embryonic cortical neuronal cultures.
Western Blot Analysis:
MAB347 will detect a single 43-48 kD band in western blots of membrane fractions of growing neurons.
Optimal working dilutions must be determined by the end user.生物信息 免疫原品种 GAP-43 purified from rat brain 克隆 9-1E12 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Mouse 特异性 GAP-43 (also known as growth associated protein-43, B-50, F1 and pp46), regardless of the protein’s phosphorylation state. 同种型 IgG1 品种反应性 HamsterHumanRatFeline Species Reactivity Note Rat, feline and hamster.
Expected to react with human based on sequence homology.抗体类型 Monoclonal Antibody Entrez基因编号 - NM_002045.2
Entrez基因汇总 The protein encoded by this gene has been termed a 'growth' or 'plasticity' protein because it is expressed at high levels in neuronal growth cones during development and axonal regeneration. This encoded protein is considered a crucial component of an effective regenerative response in the nervous system. 基因符号 - GAP-43/B50
- GAP43
- PP46
- B-50
纯化方法 Protein A purfied UniProt编号 - P17677
UniProt汇总 FUNCTION: SwissProt: P17677 # This protein is associated with nerve growth. It is a major component of the motile growth cones that form the tips of elongating axons.
SIZE: 238 amino acids; 24803 Da
SUBUNIT: Binds calmodulin with a greater affinity in the absence of Ca(2+) than in its presence (By similarity).
SUBCELLULAR LOCATION: Cell membrane; Peripheral membrane protein; Cytoplasmic side. Cell projection, growth cone membrane; Peripheral membrane protein; Cytoplasmic side. Cell junction, synapse. Note=Cytoplasmic surface of growth cone and synaptic plasma membranes.
PTM: Phosphorylation of this protein by a protein kinase C is specifically correlated with certain forms of synaptic plasticity.
SIMILARITY: SwissProt: P17677 ## Belongs to the neuromodulin family. & Contains 1 IQ domain.产品使用声明 质量保证 Routinely evaluated by Western Blot on rat brain lysates.
Western Blot Analysis:
1:1000 dilution of this lot detected GAP-43/B-50 on 10 μg of rat brain lysates.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Maintain at 2-8°C in undiluted for up to 6 months after date of receipt. 包装信息 数量 50 µg
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