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merck millipore,默克密理博,SCM015,Mesenchymal Stem Cell Expansion Medium (1x)
产品名称：Mesenchymal Stem Cell Expansion Medium (1x)
产品型号：SCM015
The Mesenchymal Stem Cell Expansion Medium (1x) has been optimized & validated for Stem cell culture. This medium is available in a 500mL format.
merck millipore,默克密理博,SCM015,Mesenchymal Stem Cell Expansion Medium (1x)
- 产品介绍
重要规格表
主要应用 Cult 描述 产品目录编号 SCM015 品牌系列 Chemicon®
商名 - Chemicon
描述 Mesenchymal Stem Cell Expansion Medium (1x) 产品信息 演示 Mesenchymal Stem Cell Expansion Medium is a proprietary formulation that contains fetal bovine serum. 应用 应用 The Mesenchymal Stem Cell Expansion Medium (1x) has been optimized & validated for Stem cell culture. This medium is available in a 500mL format. 主要应用 - Stem Cell Culture
应用说明
Thawing of Cells:
1. Do not thaw the cells until the recommended medium and appropriate plasticware and/or glassware are on hand.
2. Remove the vial of mesenchymal stem cells from liquid nitrogen and incubate in a 37°C water bath. Closely monitor until the cells are completely thawed. Maximum cell viability is dependent on the rapid and complete thawing of frozen cells. IMPORTANT: Do not vortex the cells.
3. As soon as the cells are completely thawed disinfect the outside of the vial with 70% ethanol. Proceed immediately to the next step.
4. In a laminar flow hood, use a 1 or 2 mL pipette to transfer the cells to a sterile 15 mL conical tube. Be careful to not introduce any bubbles during the transfer process.
5. Using a 10 mL pipette, slowly add dropwise 9 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed at 37°C) to the 15 mL conical tube. IMPORTANT: Do not add the whole volume of medium at once to the cells. This may result in decreased cell viability due to osmotic shock.
6. Gently mix the cell suspension by slow pipeting up and down twice. Be careful to not introduce any bubbles. IMPORTANT: Do not vortex the cells.
7. Centrifuge the tube at 300 x g for 2-3 minutes to pellet the cells.
8. Decant as much of the supernatant as possible. Steps 4-8 are necessary to remove residual cryopreservative (DMSO).
9. Resuspend the cells in a total volume of 10 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C).
10. Plate the cell mixture onto a 10-cm tissue culture plate.
11. Incubate the cells at 37°C in a 5% CO2 humidified incubator.
12. The next day, exchange the medium with fresh Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C). Exchange with fresh medium every two to three days thereafter.
13. When the cells are approximately 80% confluent, they can be dissociated with Accutase™ and passaged or alternatively frozen for later use.
Subculturing:
1. Carefully remove the medium from the 10-cm tissue culture plate containing the confluent layer of mesenchymal stem cells.
2. Apply 3-5 mL of Accutase™ and incubate in a 37°C incubator for 3 minutes.
3. Inspect the plate and ensure the complete detachment of cells by gently tapping the side of the plate with the palm of your hand. 4. Apply 5 mL of Mesenchymal Stem Cell Expansion Medium (pre-warmed to 37°C) to the plate.
5. Transfer the dissociated cells to a 15 mL conical tube.
6. Centrifuge the tube at 300 x g for 2-3 minutes to pellet the cells.
7. Discard the supernatant
8. Apply 2 mL of Mesenchymal Stem Cell Expansion Medium to the conical tube and resuspend the cells thoroughly. IMPORTANT: Do not vortex.
9. Count the number of cells using a hemacytometer.
10. Plate the cells to the desired density into the appropriate flasks, plates or wells in Mesenchymal Stem Cell Expansion. We typically plate the cells at ~2 million cells per 10-cm plate or T75 flask.产品使用声明 质量保证 Each lot of medium is tested for the optimal cell growth and proliferation of mesenchymal stem cells. Sterility Testing: Negative 使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Mesenchymal Stem Cell Expansion Medium should be stored at -20°C until ready to use. Upon thawing, the basal medium should be stored at 2-8°C for up to one month. 包装信息 数量 500 mL
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