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品种反应性 主要应用 宿主 格式 抗体类型 Chp, H, M, Rhesus Macaque, R WB Rb Purified Polyclonal Antibody 描述 产品目录编号 ABN32 描述 Anti-PDE9A Antibody Alternate Names - High affinity cGMP-specific 3',5'-cyclic phosphodiesterase 9A
背景信息 PDE9A is a ubiquitous protein belonging to the cyclic nucleotide phosphodiesterase family which regulate the signaling of cyclic nucleotides such as cGMP and cAMP. PDE9A has a higher affinity for cGMP. It contains a catalytic site that associates with cofactors and hydrolyzes cGMP thereby terminating cGMP signaling for a number of cellular processes. PDE9A has been indirectly linked to signaling pathways involved in cognitive processes. Previous studies suggested that inhibition of PDE9A enhanced long-term potentiation (a widely studied mechanism of learning and memory); PDE9A may also effect changes in the plasticity of hippocampal neurons which are also important for certain types of cognitive processes. In addition to the brain, PDE9A is also highly expressed in the spleen, prostate, colon, kidney, and heart and may be involved in a range of physiological processes in these systems, via cGMP-amplified signaling. 产品信息 格式 Purified 控制 - Human spleen tissue lysate
演示 Purified rabbit polyclonal in buffer containing 0.1 M Tris-Glycine (pH 7.4), 150 mM NaCl with 0.05% sodium azide. 应用 应用 Detect PDE9A using this Anti-PDE9A Antibody validated for use in Western Blotting, IHC(P). 主要应用 - Western Blotting
应用说明 Western Blot Analysis: A representative lot of this antibody detected PDE9A in human liver tissue lysate, and in mouse spleen and liver tissue lysates.
Immunohistochemistry Analysis: A 1:100 dilution of this antibody detected PDE9 in human prostate tissue, and in possible golgi type II and basket cells in the Purkinje and molecular layers of mouse cerebellum.生物信息 免疫原品种 KLH-conjugated linear peptide corresponding to human PDE9A. 浓缩 Please refer to the Certificate of Analysis for the lot-specific concentration. 宿主 Rabbit 品种反应性 ChimpanzeeHumanMouseRhesus MacaqueRat Species Reactivity Note Demonstrated to react with Human, Mouse, and Rhesus Macaque. Predicted to react with Rat and Chimpanzee based on 100% sequence homology. 抗体类型 Polyclonal Antibody Entrez基因编号 - NP_002597
Entrez基因汇总 The protein encoded by this gene catalyzes the hydrolysis of cAMP and cGMP to their corresponding monophosphates. The encoded protein plays a role in signal transduction by regulating the intracellular concentration of these cyclic nucleotides. Multiple transcript variants encoding several different isoforms have been found for this gene. 基因符号 - PDE9A
纯化方法 Protein A purified UniProt编号 - O76083
UniProt汇总 FUNCTION: Hydrolyzes the second messenger cGMP, which is a key regulator of many important physiological processes.
CATALYTIC ACTIVITY: Guanosine 3',5'-cyclic phosphate + H2O = guanosine 5'-phosphate.
COFACTOR: Binds 2 divalent metal cations per subunit. Site 1 may preferentially bind zinc ions, while site 2 has a preference for magnesium and/or manganese ions.
ENZYME REGULATION: Inhibited by zaprinast.
SUBCELLULAR LOCATION: Isoform PDE9A1: Cell projection > ruffle membrane. Cytoplasm > perinuclear region. Golgi apparatus. Endoplasmic reticulum. Isoform PDE9A2: Cell projection > ruffle membrane. Cytoplasm > perinuclear region. Isoform PDE9A3: Cytoplasm. Endoplasmic reticulum. Isoform PDE9A17: Cytoplasm. Endoplasmic reticulum.
TISSUE SPECIFICITY: Expressed in all tissues examined (testis, brain, small intestine, skeletal muscle, heart, lung, thymus, spleen, placenta, kidney, liver, pancreas, ovary and prostate) except blood. Highest levels in brain, heart, kidney, spleen, prostate and colon. Isoform PDE9A12 is found in prostate.
DOMAIN: Composed of a C-terminal catalytic domain containing two putative divalent metal sites and an N-terminal regulatory domain.
SEQUENCE SIMILARITIES: Belongs to the cyclic nucleotide phosphodiesterase family. PDE9 subfamily.产品使用声明 质量保证 Evaluated by Western Blot in human spleen tissue lysate.
Western Blot Analysis: 0.5 µg/mL of this antibody detected PDE9A in 10 µg of human spleen tissue lysate.使用声明 - Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
储存和货运信息 存储条件 Stable for 1 year at 2-8°C from date of receipt. 包装信息 数量 100 µg
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